Real-time circulating tumor cells detection via highly sensitive needle-like cytosensor-demonstrated by a blood flow simulation

Weng, Wen‐Hui; Ho, Ing-Kang; Pang, Chi-Chia; Pang, Sow-Neng; Pan, Tung-Ming; Leung, Wai-Hung

doi:10.1016/j.bios.2018.05.035

Cited by 15 publications

(8 citation statements)

References 28 publications

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“…Meanwhile, a cutoff of ≥5 cells/7.5 mL blood is more frequently used in other cancer types 36,37 . In the present study, we have tested multiple cutoff values of CTC counts, and three cutoffs (≥1.0 38,39 , ≥5.0 39 , ≥20.0 40 cells/mL blood) with reported clinical significance in the literature were found to be significant in the association with clinicopathological characteristics (Table 2 and Fig. S1).…”

Section: Discussionmentioning

confidence: 99%

The Prognostic Value of Circulating Tumor Cells in Asian Neuroendocrine Tumors

Hsieh

Chen

Jhou

et al. 2019

Sci Rep

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Circulating tumor cells (CTC) play important roles in various cancers; however, few studies have assessed their clinical utility in neuroendocrine tumors. This study aimed to prospectively evaluate the prognostic value of CTC counts in Asian patients with neuroendocrine tumors before and during anti-cancer therapy. Patients who were diagnosed with unresectable histological neuroendocrine tumors between September 2011 and September 2017 were enrolled. CTC testing was performed before and during anti-cancer therapy using a negative selection protocol. Chromogranin A levels were also assessed. Univariate and multivariate Cox’s proportional hazard model with forward LR model was performed to investigate the impact of independent factors on overall survival and progression-free survival. Kaplan–Meier method with log-rank tests were used to determine the difference among different clinicopathological signatures and CTC cutoff. The baseline CTC detection rate was 94.3% (33/35). CTC counts were associated with cancer stages (I-III vs. IV, P = 0.015), liver metastasis (P = 0.026), and neuroendocrine tumor grading (P = 0.03). The median progression-free survival and overall survivals were 12.3 and 30.4 months, respectively. In multivariate Cox regression model, neuroendocrine tumors grading and baseline CTC counts were both independent prognostic factors for progression-free survival (PFS, P = 0.005 and 0.015, respectively) and overall survival (OS, P = 0.018 and 0.023, respectively). In Kaplan-Meier analysis, lower baseline chromogranin A levels were associated with longer PFS (P = 0.024). Baseline CTC counts are associated with the clinicopathologic features of neuroendocrine tumors and are an independent prognostic factor for this malignancy.

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Section: Discussionmentioning

confidence: 99%

The Prognostic Value of Circulating Tumor Cells in Asian Neuroendocrine Tumors

Hsieh

Chen

Jhou

et al. 2019

Sci Rep

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“…Heat treatment after silanization is another important parameter to consider since it affects the covalent bond forming between APTES molecule and the surface. Two heat treatment temperatures (70 °C [ 4 ] and 130 °C [ 27 ]) were tested for HT after electrodeposition ( Figure 6 A,B) taken as the lowest and the highest reported in the literature. As a result, it was observed that at 130 °C, a more uniform layer was formed, while for 70 °C, some larger particles (up to 4.5 nm) were seen.…”

Section: Resultsmentioning

confidence: 99%

“…Stainless steel (SS) is commonly used in biomedical devices and applications because of its biocompatibility and mechanical properties [ 1 , 2 ]. Recent applications of SS include functionalized guidewire for the capture of cells [ 3 ], biosensing [ 4 ] and sorptive extraction of contaminants [ 5 ]. All of these applications require chemical modification of the surface.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, capturing CTCs using this device and further molecular characterization of the cells could bring personalized medicine closer to reality [ 12 , 21 ]. Other devices designed to isolate CTCs from the blood stream include flexible magnetic wires that capture cells previously labelled with injected magnetic nanoparticles [ 22 ] and a “cytosensor” based on the functionalized SS needle (also covered with EpCAM antibodies) [ 4 ]. All of these studies, however, rely on the detection of CTC by EpCAM antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation

et al. 2020

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Chemically modified metal surfaces have been used to recognize and capture specific cell types and biomolecules. In this work, stainless steel wires were functionalized with aptamers against breast cancer stem cell markers. Stainless steel wires were first electropolished and silanized via electrodeposition. Aptamers were then attached to the silanized surface through a cross-linker. The functionalized wires were able to capture the target cells in an in vitro test. During surface modification steps, wires were analyzed by atomic force microscopy, cyclic voltammetry, scanning electron and fluorescence microscopy to determine their surface composition and morphology. Optimized conditions of silanization (applied potential, solution pH, heat treatment temperature) for obtaining an aptamer-functionalized wire were determined in this work together with the use of several surface characterization techniques suitable for small-sized and circular wires. These modified wires have potential applications for the in vivo capture of target cells in blood flow, since their small size allows their insertion as standard guidewires in biomedical devices.

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“…Carcinoembryonic antigen (CEA) [ 6 ] and carbohydrate antigen 19–9 (CA 19–9) [ 9 ] are the two most-used cancer antigens for monitoring colon cancer [ 10 ]. Circulating tumor cells originate from the primary or metastatic sites of colon cancer, and can directly reveal the status of the disease, however, the number of circulating tumor cells is very low [ 11 ]. DNA and RNA-based molecular method is more sensitive and useful than circulating tumor cells for the investigation of molecular heterogeneity and clonal divergence of colon cancer; but those molecular markers are still not utilized in clinic.…”

Section: Introductionmentioning

confidence: 99%

Combination of serum lipids and cancer antigens as a novel marker for colon cancer diagnosis

Qian²,

et al. 2018

Lipids Health Dis

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BackgroundColon cancer is a malignancy of the large intestine with high mortality and economic burden. Recent studies reveal a new relationship between blood lipids and the risk of cancer. The presents study aims to investigate the combination of serum lipids with cancer antigens as a novel diagnostic marker for colon cancer.MethodsTwo hundred of colon cancer patients or healthy subjects were recruited. Serum lipids and cancer antigens such as total cholesterol (TC), high-density lipoprotein (HDL), carcinoembryonic antigen (CEA) and carbohydrate antigen 19–9 (CA19–9) were measured.ResultsThere were significantly lower level of serum TC or HDL, and significantly higher level of serum CEA or CA19–9 in patients than in healthy subjects. Serum TC or HDL in patients with advanced colon cancer was significantly lower than the ones with early stage disease. The level of serum TC or HDL in patients after surgical removal of colon cancer was significantly higher compared to the ones before surgery, but serum CEA or CA19–9 after surgery was significantly reduced in comparison with the ones before surgery. Combined TC, HDL, CEA and CA19–9 as a diagnostic marker for colon cancer had the highest positive predictive rate in comparison with individual, two or three of the parameters.ConclusionsThe combination of serum TC, HDL, CEA and CA19–9 can be used as an effective marker for colon cancer, and offers a novel strategy for clinical diagnosis and monitoring the disease.

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Real-time circulating tumor cells detection via highly sensitive needle-like cytosensor-demonstrated by a blood flow simulation

Cited by 15 publications

References 28 publications

The Prognostic Value of Circulating Tumor Cells in Asian Neuroendocrine Tumors

The Prognostic Value of Circulating Tumor Cells in Asian Neuroendocrine Tumors

Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation

Combination of serum lipids and cancer antigens as a novel marker for colon cancer diagnosis

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