Real-time cell-microelectronic sensing of nanoparticle-induced cytotoxic effects

Moe, Birget; Gabos, Stephan; Li, Xing‐Fang

doi:10.1016/j.aca.2013.06.002

Cited by 35 publications

(30 citation statements)

References 37 publications

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“…However, TiO 2 NPs increased in S phase in bronchial epithelial cells when NPs were dispersed in KF medium (Prasad et al 2013). An increase in S phase was determined in ovary cells (Moe et al 2013) and neuroblastoma (Valdiglesias et al 2013) exposed to TiO 2 NPs. Nevertheless, proliferation index of mesenchymal stem cells decreased after 3 days of TiO 2 NP exposure in mesenchymal stem cells ) and G0/G1 arrest in synovial TiO 2 NP-exposed cells ).…”

Section: Introductionmentioning

confidence: 99%

Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles

Medina‐Reyes

Bucio-López

Freyre-Fonseca

et al. 2014

Environ Sci Pollut Res

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Titanium dioxide has been classified in the 2B group as a possible human carcinogen by the International Agency for Research on Cancer, and amid concerns of its exposure, cell cycle alterations are an important one. However, several studies show inconclusive effects, mainly because it is difficult to compare cell cycle effects caused by TiO2 nanoparticle (NP) exposure between different shapes and sizes of NP, cell culture types, and time of exposure. In addition, cell cycle is frequently analyzed without cell cycle synchronization, which may also mask some effects. We hypothesized that synchronization after TiO2 NP exposure could reveal dissimilar cell cycle progression when compared with unsynchronized cell population. To test our hypothesis, we exposed lung epithelial cells to 1 and 10 μg/cm(2) TiO2 NPs for 7 days and one population was synchronized by serum starvation and inhibition of ribonucleotide reductase using hydroxyurea. Another cell population was exposed to TiO2 NPs under the same experimental conditions, but after treatments, cell cycle was analyzed without synchronization. Our results showed that TiO2 NP-exposed cells without synchronization had no changes in cell cycle distribution; however, cell population synchronized after 1 and 10 μg/cm(2) TiO2 NP treatment showed a 1.5-fold and 1.66-fold increase, respectively, in proliferation. Synchronized cells also reveal a faster capability of TiO2 NP-exposed cells to increase cell population in the G2/M phase in the following 9 h after synchronization. We conclude that synchronization discloses a greater percentage of cells in the G2/M phase and higher proliferation than TiO2 NP-synchronized cells.

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Section: Introductionmentioning

confidence: 99%

Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles

Medina‐Reyes

Bucio-López

Freyre-Fonseca

et al. 2014

Environ Sci Pollut Res

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“…The authors think that nanoparticle agglomeration leads to their size variation, which might be due to various ions (including Na + , Cl − , K + , PO 3 − etc.) in RPMI1640 medium [28,29]. These ions alter the intermolecular forces on the surface of nanoparticles, leading to higher non-covalent bindings.…”

Section: Discussionmentioning

confidence: 98%

Surface modification of tri-calcium phosphate nanoparticles by DOPE and/or anti-E6 antibody to enhance uptake of antisense of E6 mRNA

Jebali

Kalantar

Hekmatimoghaddam

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…Impedance measurements have been used in a number of different studies to determine morphological changes in real time;27 cisplatin-induced cell death;28 differentiation of 3T3 cells into adipocytes;29 effects of curcumin on physicochemical characterization and effects on MCF7 cancer cell proliferation;30 monitoring of dynamic interactions of tumor cells with tissues and immune cells on a lab-on-a-chip;31 cell senescence measurements of adipose tissue-derived stem cells;32 and real-time evaluation of nanoparticle-induced cytotoxic effects 33. By this method, Moodley et al34 conducted real-time profiling of NK cell killing of human astrocytes.…”

Section: Discussionmentioning

confidence: 99%

A simple in vitro method for evaluating dendritic cell-based vaccinations

Pham¹,

Nguyen²,

Nguyen³

et al. 2014

OTT

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BackgroundDendritic cell (DC) therapy is a promising therapy for cancer-targeting treatments. Recently, DCs have been used for treatment of some cancers. We aimed to develop an in vitro assay to evaluate DC therapy in cancer treatment using a breast cancer model.MethodsDCs were induced from murine bone marrow mononuclear cells in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with GM-CSF (20 ng/mL) and IL-4 (20 ng/mL). Immature DCs were primed with breast cancer stem cell (BCSC)-derived antigens. BCSCs were sorted from 4T1 cell lines based on aldehyde dehydrogenase expression. A mixture of DCs and cytotoxic T lymphocytes (CTLs) were used to evaluate the inhibitory effect of antigen-primed DCs on BCSCs. BCSC proliferation and doubling time were recorded based on impedance-based cell analysis using the xCELLigence system. The specification of inhibitory effects of DCs and CTLs was also evaluated using the same system.ResultsThe results showed that impedance-based analysis of BCSCs reflected cytotoxicity and inhibitory effects of DCs and CTLs at 72 hours. Differences in ratios of DC:CTL changed the cytotoxicity of DCs and CTLs.ConclusionThis study successfully used impedance-based cell analysis as a new in vitro assay to evaluate DC efficacy in cancer immunotherapy. We hope this technique will contribute to the development and improvement of immunotherapies in the near future.

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Real-time cell-microelectronic sensing of nanoparticle-induced cytotoxic effects

Cited by 35 publications

References 37 publications

Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles

Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles

Surface modification of tri-calcium phosphate nanoparticles by DOPE and/or anti-E6 antibody to enhance uptake of antisense of E6 mRNA

A simple in vitro method for evaluating dendritic cell-based vaccinations

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