Real-time assessment of high-intensity focused ultrasound heating and cavitation with hybrid optoacoustic ultrasound imaging

Özsoy, Çağla; Lafci, Berkan; Reiß, Michael; Deán‐Ben, Xosé Luís; Razansky, Daniel

doi:10.1016/j.pacs.2023.100508

Cited by 8 publications

(4 citation statements)

References 93 publications

(137 reference statements)

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“…Results showed a uniform distribution of heat across the focal region in both cell configurations. However, approximately 12.5 % higher maximum intensity was recorded at the same applied pressure in spheroids than in monolayers, which increased as the applied pressure was also increased (47). This can potentially be due to an additional absorption coefficient and specific heat capacity offered by the hydrogel to spheroids (Table 1), causing the latter to absorb much more heat than the hydrogel-lacking monolayers, while only leading to slow increase in temperature.…”

Section: Monolayer Vs Spheroid Ablation Temperaturementioning

confidence: 97%

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Abu

et al. 2023

Preprint

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ObjectiveThe primary objective of our study was to investigate the efficiency of high intensity focused ultrasound (HIFU) ablation in two distinct cellular configurations, 2D monolayers and 3D spheroids of epithelial breast cancer cell lines. The study also compares empirical findings from experiments with results obtained through numerical simulations using a bioheat computational model. This comparison is intended to provide a comprehensive understanding of the acoustic energy conversion within the biological system during HIFU treatment.MethodsHIFU was applied to 2D and 3D cultured MDA-MB 231 and MCF7 epithelial breast cancer cell lines while systematically varying ultrasound intensity and duty cycle (DC) during sonication sessions of different durations. Temperature elevation was measured and the ablation percentage was calculated based on bright field and fluorescent imaging of the treated regions. Experimental results were validated through simulations of the ablation setup.ResultsUpon HIFU, spheroids exhibited a lower temperature increase (approximately 20 °C) when subjected to comparable acoustic intensities and duty cycles. The level of tumor ablation was highly influenced by DC, with higher DCs leading to greater ablation percentages. However, sonication duration had a minimal impact on the degree of ablation. Numerical simulations corroborated these observations, demonstrating uniform heat distribution within the cultured cells. At higher DCs and intensities, complete ablation of spheroids was achieved, whereas at lower levels, only the outermost layers exhibited ablation.ConclusionOur study reveals a significant disparity in the response of 2D monolayers and 3D spheroids to HIFU treatment. Specifically, tumor spheroids require lower temperature elevations for effective ablation, and their ablation percentage significantly increases with elevated DC.

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Section: Monolayer Vs Spheroid Ablation Temperaturementioning

confidence: 97%

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Abu

et al. 2023

Preprint

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show abstract

“…The results showed a uniform distribution of heat across the focal region in both cell configurations. However, approximately 12.5% higher maximum intensity was recorded at the same applied pressure in spheroids than in monolayers, which increased as the applied pressure was also increased [60]. This can potentially be due to an additional absorption coefficient and specific heat capacity offered by the hydrogel to spheroids (Table 1), causing the latter to absorb much more heat than the hydrogel-lacking monolayers while only lead-ing to a slow increase in temperature.…”

Section: Monolayer Vs Spheroid Ablation Temperaturementioning

confidence: 97%

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Raad

et al. 2024

Cancers

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High-intensity focused ultrasound (HIFU) is a non-invasive therapeutic modality that uses precise acoustic energy to ablate cancerous tissues through coagulative necrosis. In this context, we investigate the efficacy of HIFU ablation in two distinct cellular configurations, namely 2D monolayers and 3D spheroids of epithelial breast cancer cell lines (MDA-MB 231 and MCF7). The primary objective is to compare the response of these two in vitro models to HIFU while measuring their ablation percentages and temperature elevation levels. HIFU was systematically applied to the cell cultures, varying ultrasound intensity and duty cycle during different sonication sessions. The results indicate that the degree of ablation is highly influenced by the duty cycle, with higher duty cycles resulting in greater ablation percentages, while sonication duration has a minimal impact. Numerical simulations validate experimental observations, highlighting a significant disparity in the response of 2D monolayers and 3D spheroids to HIFU treatment. Specifically, tumor spheroids require lower temperature elevations for effective ablation, and their ablation percentage significantly increases with elevated duty cycles. This study contributes to a comprehensive understanding of acoustic energy conversion within the biological system during HIFU treatment for 2D versus 3D ablation targets, holding potential implications for refining and personalizing breast cancer therapeutic strategies.

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“…The thermal effect causes a local temperature rise that leads to irreversible cell death through coagulative necrosis. The mechanical effect, also called cavitation, causes irreversible damage by mechanically disrupting cell membrane permeability and altering the structure of cells [ 3 ]. In the past several decades, HIFU has been clinically used to treat bone, breast, kidney, liver, pancreas, and prostate cancer [ 4 , 5 , 6 , 7 , 8 , 9 ]; uterine fibroids [ 10 ]; and cardiac arrhythmias [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, US imaging has relatively low-imaging contrast, sensitivity, and specificity for non-invasive detection [ 29 ]. Although US imaging can also provide thermometry by measuring local changes in speed of sound, its sensitivity and accuracy of thermometry are relatively low becuase of the complex physical nature of acoustic tissue properties and mechanisms of cavitation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Deep-Learning-Based High-Intensity Focused Ultrasound Lesion Segmentation in Multi-Wavelength Photoacoustic Imaging

Wu,

Sanders,

Dundar

et al. 2023

Bioengineering

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Photoacoustic (PA) imaging can be used to monitor high-intensity focused ultrasound (HIFU) therapies because ablation changes the optical absorption spectrum of the tissue, and this change can be detected with PA imaging. Multi-wavelength photoacoustic (MWPA) imaging makes this change easier to detect by repeating PA imaging at multiple optical wavelengths and sampling the optical absorption spectrum more thoroughly. Real-time pixel-wise classification in MWPA imaging can assist clinicians in monitoring HIFU lesion formation and will be a crucial milestone towards full HIFU therapy automation based on artificial intelligence. In this paper, we present a deep-learning-based approach to segment HIFU lesions in MWPA images. Ex vivo bovine tissue is ablated with HIFU and imaged via MWPA imaging. The acquired MWPA images are then used to train and test a convolutional neural network (CNN) for lesion segmentation. Traditional machine learning algorithms are also trained and tested to compare with the CNN, and the results show that the performance of the CNN significantly exceeds traditional machine learning algorithms. Feature selection is conducted to reduce the number of wavelengths to facilitate real-time implementation while retaining good segmentation performance. This study demonstrates the feasibility and high performance of the deep-learning-based lesion segmentation method in MWPA imaging to monitor HIFU lesion formation and the potential to implement this method in real time.

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Real-time assessment of high-intensity focused ultrasound heating and cavitation with hybrid optoacoustic ultrasound imaging

Cited by 8 publications

References 93 publications

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Deep-Learning-Based High-Intensity Focused Ultrasound Lesion Segmentation in Multi-Wavelength Photoacoustic Imaging

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