Real-life evaluation of a rapid antigen test (DPP SARS-CoV-2 Antigen) for COVID-19 diagnosis of primary healthcare patients, in the context of the Omicron-dominant wave in Brazil

Bezerra, Matheus Filgueira; Silva, Lílian Caroliny Amorim; Silva, Rômulo Pessoa e; Lino, Gisele; Soares, Filipe Zimmer Dezordi; Lima, Gustavo Barbosa de; Lima, Raul Emídio de; Campos, Túlio de Lima; Docena, Cássia; Oliveira, Anderson Bruno de; Pitta, Maíra Galdino da Rocha; Santos, Francisco de Assis da Silva; Pereira, Michelly Cristiny; Wallau, Gabriel Luz; Paiva, Marcelo Henrique Santos

doi:10.1016/j.cmi.2022.11.003

Cited by 2 publications

(4 citation statements)

References 9 publications

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“…The average was not obtained as the sample size was not known for each date. In Brazil, the prevalence of the Omicron variant increased from 0.4% between December 2021–2022 (n = 241) 25 to 85% between February and March 2022 (n = 67) 64 while in Ecuador the prevalence was 50% in December 2021 (n = 66). 56 Only one study reported the prevalence of the Omicron variant in Chile as 90.8% between November 2021 and January 2022 ( Figure 3(a) ).…”

Section: Resultsmentioning

confidence: 99%

“… 77 However, BA.2 predominated from March to June 2022 while BA.4 and BA.5 started to emerge. 62 , 78 In Brazil, BA.1 predominated between December 2021 to April 2022 while BA.2 started to appear 64 , 80 ( Figure 4(a) ). Similarly, BA.1 predominated in South Africa over BA.2 and BA.5 emerged between October 2021 and April 2022) 22 , 61 ( Figure 4(b) ).…”

Section: Resultsmentioning

confidence: 99%

“… 62 , 78 In Brazil, BA.1 predominated between December 2021 to April 2022 while BA.2 started to appear. 64 , 79 Similarly, BA.1 predominated in South Africa over BA.2 and BA.5 started to appear between October 2021 and April 2022. 22 , 61 …”

Section: Discussionmentioning

confidence: 97%

“…Likewise, the prevalence of the Omicron variant increased in Brazil from 0.4% between December 2021–2022, 25 to 85% between February and March 2022. 64 However, both studies had small cohorts of 241 and 67 individuals, respectively. Another sharp increase was reported in Brazil by Ranzani et al, 79 who reported the increase in the prevalence of Omicron from 1% (September–December 2021) to 100% (December 2021-April 2022).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The relative prevalence of the Omicron variant within SARS-CoV-2 infected cohorts in different countries: A systematic review

Sarkar,

Omar,

Alshareef

et al. 2023

Human Vaccines & Immunotherapeutics

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The Omicron variant of SARS-CoV-2 was detected in October 2021 and exhibited high transmissibility, immune evasion, and reduced severity when compared to the earlier variants. The lesser vaccine effectiveness against Omicron and its reduced severity created vaccination hesitancy among the public. This review compiled data reporting the relative prevalence of Omicron as compared to the early variants to give an insight into the existing variants, which may shape the decisions regarding the targets of the newly developed vaccines. Complied data revealed more than 90% prevalence within the infected cohorts in some countries. The BA.1 subvariant predominated over the BA.2 during the early stages of the Omicron wave. Moreover, BA.4/BA.5 subvariants were detected in South Africa, USA and Italy between October 2021 and April 2022. It is therefore important to develop vaccines that protect against Omicron as well as the early variants, which are known to cause more severe complications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The relative prevalence of the Omicron variant within SARS-CoV-2 infected cohorts in different countries: A systematic review

Sarkar,

Omar,

Alshareef

et al. 2023

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

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A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses

Ferreira da Silva,

Valle Garay,

Queiroz

et al. 2024

PLoS ONE

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The diagnoses of retroviruses are essential for controlling the rapid spread of pandemics. However, the real-time Reverse Transcriptase quantitative Polymerase Chain Reaction (RT-qPCR), which has been the gold standard for identifying viruses such as SARS-CoV-2 in the early stages of infection, is associated with high costs and logistical challenges. To innovate in viral RNA detection a novel molecular approach for detecting SARS-CoV-2 viral RNA, as a proof of concept, was developed. This method combines specific viral gene analysis, trans-acting ribozymes, and Fluorescence Resonance Energy Transfer (FRET)-based hybridization of fluorescent DNA hairpins. In this molecular mechanism, SARS-CoV-2 RNA is specifically recognized and cleaved by ribozymes, releasing an initiator fragment that triggers a hybridization chain reaction (HCR) with DNA hairpins containing fluorophores, leading to a FRET process. A consensus SARS-CoV-2 RNA target sequence was identified, and specific ribozymes were designed and transcribed in vitro to cleave the viral RNA into fragments. DNA hairpins labeled with Cy3/Cy5 fluorophores were then designed and synthesized for HCR-FRET assays targeting the RNA fragment sequences resulting from ribozyme cleavage. The results demonstrated that two of the three designed ribozymes effectively cleaved the target RNA within 10 minutes. Additionally, DNA hairpins labeled with Cy3/Cy5 pairs efficiently detected target RNA specifically and triggered detectable HCR-FRET reactions. This method is versatile and can be adapted for use with other viruses. Furthermore, the design and construction of a DIY photo-fluorometer prototype enabled us to explore the development of a simple and cost-effective point-of-care detection method based on digital image analysis.

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Real-life evaluation of a rapid antigen test (DPP SARS-CoV-2 Antigen) for COVID-19 diagnosis of primary healthcare patients, in the context of the Omicron-dominant wave in Brazil

Cited by 2 publications

References 9 publications

The relative prevalence of the Omicron variant within SARS-CoV-2 infected cohorts in different countries: A systematic review

The relative prevalence of the Omicron variant within SARS-CoV-2 infected cohorts in different countries: A systematic review

A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses

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