To understand the characteristics of the transcriptional group of Taenia pisiformis at 14 different developmental stages, and to lay the foundation for the screening of vaccine antigens and 15 drug target genes, the transcriptomes of adult and larva of T. pisiformis were assembled and 16 analyzed using bioinformatic tools. A total of 36,951 unigenes with a mean length of 950bp were 17 formed, among which 12,665, 8,188, 7,577, and 6,293 unigenes have been annotated respectively by 18 sequence similarity analysis with four databases (NR, Swiss-Prot, KOG, and KEGG). It should be 19 noted there are 5,662 unigenes that share good similarity with the four databases and get a 20 relatively perfect functional annotation. Besides, a total of 10,247 differentially expressed genes 21 were screened. To be specific, 6,910 unigenes were up-regulated in the larva stage while 3,337 22 were down-regulated in the adult stage. To sum up, this study sequenced and analyzed the 23 transcriptomes of the larval and adult stages of T. pisiformis. The results of differentially expressed 24 genes in these two stages could provide basis for functional genomics, immunology and gene 25 expression profiles of T. pisiformis.26