Reagentless detection of microorganisms by intrinsic fluorescence

Estes, C.; Duncan, A.; Wade, B.; Lloyd, Christopher R.; Ellis, Walther R.; Powers, Linda S.

doi:10.1016/s0956-5663(03)00008-3

Cited by 67 publications

(42 citation statements)

References 13 publications

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“…Fluorescence and Raman spectroscopies are among the most promising nondestructive methods for confirmatory identification of body fluids [4][5][6][7][8][9][10][11][12]. The less sensitive Raman spectroscopy in comparison with fluorescence has higher selectivity and specificity to biochemical species and could potentially be useful in resolving mixtures of multiple body fluids with minimal sample preparation or manipulation [4][5][6][7][8][9][10][11]13].…”

Section: Open Accessmentioning

confidence: 99%

Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes

Sikirzhytski

Virkler

Lednev

et al. 2010

AIP Conference Proceedings

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Detection and identification of blood, semen and saliva stains, the most common body fluids encountered at a crime scene, are very important aspects of forensic science today. This study targets the development of a nondestructive, confirmatory method for body fluid identification based on Raman spectroscopy coupled with advanced statistical analysis. Dry traces of blood, semen and saliva obtained from multiple donors were probed using a confocal Raman microscope with a 785-nm excitation wavelength under controlled laboratory conditions. Results demonstrated the capability of Raman spectroscopy to identify an unknown substance to be semen, blood or saliva with high confidence.

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Section: Open Accessmentioning

confidence: 99%

Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes

Sikirzhytski

Virkler

Lednev

et al. 2010

AIP Conference Proceedings

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“…[9][10][11][12][13][14]27 The instrument used in this study was speci¯cally designed for the measurement of surfaces in the¯eld, and is diagrammatically shown in Fig. 1.…”

Section: Multiwavelength Intrinsic°u Orescence Instrumentmentioning

confidence: 99%

Real-time in situ detection and quantification of bacteria in the Arctic environment

Powers

Ellis

Lloyd³

2014

J. Innov. Opt. Health Sci.

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At present, there are no methods that determine the total microbial load on an abiotic substrate in real time. The utility of such a capability ranges from sterilization and medical diagnostics to the search for new microorganisms in the environment and study of their ecological niches. We report the development of a hand-held,°uorescence detection device and demonstrate its applicability to the¯eld detection of Arctic bacteria. This technology is based on the early pioneering work of Britton Chance which elucidated the intrinsic°uorescence of a number of metabolites and protein cofactors in cells, including reduced pyridine nucleotides, cytochromes and°avins. A PDA controls the device (°uorescence excitation and data collection) and processes the multiwavelength signals to yield bacterial cell counts, including estimates of live cells, dead cells and endospores. Unlike existing methods for cell counting, this method requires no sample contact or addition of reagents. The use of this technology is demonstrated with in situ measurements of two sub-glacial microbial communities at sites in Palander and colonized surface rocks in the Bockfjord Volcanic Complex during AMASE 2008 (Arctic Mars Analog Svalbard Expedition). The total bacterial load on the interrogated sample surfaces ranged from < 20 cells/ cm 2 to > 10 9 cells/cm 2 .

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“…Driven by the importance for forensic applications, body fluid identification methods have been extensively developed in recent years [6][7][8][9] .…”

Section: Detection Of Biological Evidencementioning

confidence: 99%

Photonics for Forensic Applications

Tajani

2013

Photonics for Safety and Security

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This chapter illustrates a few of the numerous techniques, based on electromagnetic waves, employed in forensic science investigations by Reparto Carabinieri Investigazioni Scientifiche in Rome. Although it is not possible to provide an exhaustive description of the large number of examples where light sources can be used for forensic applications, the reader will hopefully gain an appreciation of the complex nature of this fascinating field, the current state of knowledge and the areas where research remains to be done.

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Reagentless detection of microorganisms by intrinsic fluorescence

Cited by 67 publications

References 13 publications

Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes

Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes

Real-time in situ detection and quantification of bacteria in the Arctic environment

Photonics for Forensic Applications

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