Reagentless biosensor for isocitrate using one step modified Pt-Ir microelectrode

Pereira, Aline Machado

doi:10.1016/s0039-9140(00)00566-x

Cited by 16 publications

(9 citation statements)

References 16 publications

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“…This behavior can be explained by the energy necessary to oxidize the mediator excess, damaging the formation of the film. These resulted were observed also in the work developed for Pereira et al 55 On the other hand for 10 min immersed electrode the values of current intensity increased proportionally with the NADH concentration and presented two linear range: (1 st fit -lower concentration and 2 nd fit -high concentrations) (Figure 4). The sensitivity was defined by slope of the fitted line as 3.0×10 -3 and 1.0×10 -3 μA per mol L and R was 0.995 and 0.969 for lower and high concentrations, respectively.…”

Section: Stability Of the Film Formed By Sol-gel Methodssupporting

confidence: 81%

“…The PPy/MB modified electrode surface was prepared following the literature procedure, 55 which describes the use of Pt-Ir microelectrode, as work electrode and the mediator MB, concluding that the film formed above 0.600V would provides the superoxidation of the polypyrrol provoking bad definition in the signal of oxidation of the present mediator in the film.…”

Section: Modification Of the Glassy Carbon Electrode Surface By Electmentioning

confidence: 99%

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Strategies for developing NADH detector based on meldola blue in different immobilization methods: a comparative study

Mariotti¹,

Riccardi²,

Fertonani³

et al. 2006

J. Braz. Chem. Soc.

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Foi realizado um estudo comparativo entre duas metodologias de imobilização do Azul de Meldola (MB), utilizado para diminuir o potencial de oxidação do NADH. O estudo foi realizado no eletrodo de carbono vítreo através da eletropolimerização do pirrol e MB, empregando a técnica de voltametria cíclica e avaliando-se o efeito do número de ciclos para definir a espessura e a estabilidade do filme. Com 25 ciclos foi obtido o melhor resultado. Foi também investigada a modificação do eletrodo de grafite com filme formado pelo sistema sol-gel PPO 300 ou PPO 4000 pelo método de recobrimento por mergulho, através de dois métodos: oclusão e adsorção. O melhor método foi o de adsorção da MB durante 10 min, seguida de depósito do filme PPO 300, favorecido pelo tempo de secagem (24 horas). A adsorção da MB seguido do depósito do PPO 300 apresentou maior intervalo linear do que a metodologia do PPY.A comparative study was accomplished between two immobilization methodologies for the meldola's blue (MB), used to decrease the oxidation potential of NADH. The study was performed with glassy carbon electrode by electropolymerization of pyrrole and MB through the technique of cyclic voltammetry being evaluated the effect of the number of cycles to define the thickness and the stability of the film. With 25 cycles it was obtained the best result. They were also investigated the modification of the graphite electrode with film formed by the system sol-gel PPO 300 or PPO 4000 for the dip-coating, through two methods: occlusion and adsorption. The best method was it of adsorption of MB during 10 min, following by deposit of the film PPO 300, favored for the time of drying (24 hours). The adsorption of MB followed by PPO 300 deposition presented broader linear range than PPy methodology

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Section: Stability Of the Film Formed By Sol-gel Methodssupporting

confidence: 81%

Section: Modification Of the Glassy Carbon Electrode Surface By Electmentioning

confidence: 99%

Strategies for developing NADH detector based on meldola blue in different immobilization methods: a comparative study

Mariotti¹,

Riccardi²,

Fertonani³

et al. 2006

J. Braz. Chem. Soc.

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“…Various strategies, e.g. adsorption [14,15], electropolymerization [16] and entrapment in a polymer matrix [17,18], have been adopted to anchor MB on the electrode surfaces. Among them, the direct adsorption of MB on carbon surfaces (e.g.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

et al. 2009

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An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldolas blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1 AE 0.25 mA/ mM) and gave a low detection limit of 0.21 AE 0.02 mM. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1 AE 0.58 mM as well as a high sensitivity of 34.58 AE 2.43 nA/mM without suffering any interference from some common electroactive compounds.

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“…The latter retained their antigenic activity and were used for immunosensing of antibodies recognizing Rhesus blood group antigen. The co-immobilization of enzyme (isocitrate dehydrogenase), coenzyme (NADP + ), and redox mediator (Meldola's blue) was also successfully carried out on a Pt-Ir microelectrode (150 µm in diameter) [29]. In addition, it was possible to optimize the resulting microbiosensor configuration for isocitrate determination in terms of film thickness, amount of immobilized mediator, and enzyme and coenzyme loading.…”

Section: Use Of Conducting Polymer Filmsmentioning

confidence: 99%

Biosensors based on electropolymerized films: new trends

Cosnier

2003

Analytical and Bioanalytical Chemistry

255

119

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Electropolymerized films have received considerable attention in the development of biosensors and biochips, and are advancing rapidly. This paper reviews recent advances and scientific progress in electrochemical immobilization procedures for biological macromolecules on electrodes via electrogenerated polymer films. Biomolecule immobilization is classified as covalent linkage, attachment by affinity interactions, and physical entrapment. The last approach entails the use of conducting and non-conducting films, composite polymer films, and templates for the electropolymerization process. Some advances in the electrochemical transduction of biological events (enzymatic reaction, immunoreaction, or oligonucleotide hybridization) involving the redox properties or the conductivity of electropolymerized films are also presented.

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Reagentless biosensor for isocitrate using one step modified Pt-Ir microelectrode

Cited by 16 publications

References 16 publications

Strategies for developing NADH detector based on meldola blue in different immobilization methods: a comparative study

Strategies for developing NADH detector based on meldola blue in different immobilization methods: a comparative study

Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

Biosensors based on electropolymerized films: new trends

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