Reagentless Affimer- and antibody-based impedimetric biosensors for CEA-detection using a novel non-conducting polymer

Shamsuddin, Shazana Hilda; Gibson, Timothy D.; Tomlinson, Darren; McPherson, Michael J.; Jayne, David; Millner, Paul A.

doi:10.1016/j.bios.2021.113013

Cited by 37 publications

(23 citation statements)

References 43 publications

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“…Anti-CEA specific Affimer clones were identified by using a “phage display library” method as recently published by Shamsuddin et al 35 Out of the three CEA binding Affimers identified, clone II and III were chosen for this study having 9 and 10 distinct amino acid residues at the variable region, respectively, in clone II and clone III. Based on a 50 mL of working volume, the yield of the Affimers were noted to be 8.3 and 6.27 mg for clone II and III, respectively, 35 , 36 with corresponding molecular weights noted to be 12.5 and 12.6 kDa ( Figure S4A ). The associated DNA from the positive clones were sequenced.…”

Section: Materials and Methodsmentioning

confidence: 99%

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Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models

Pramanik

Shamsuddin

et al. 2022

ACS Appl. Mater. Interfaces

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Nanomedicines, while having been approved for cancer therapy, present many challenges such as low stability, rapid clearance, and nonspecificity leading to off-target toxicity. Cubosomes are porous lyotropic liquid crystalline nanoparticles that have shown great premise as drug delivery vehicles; however, their behavior in vivo is largely underexplored, hindering clinical translation. Here, we have engineered cubosomes based on the space group Im 3 m that are loaded with copper acetylacetonate as a model drug, and their surfaces are functionalized for the first time with Affimer proteins via copper-free click chemistry to actively target overexpressed carcinoembryonic antigens on LS174T colorectal cancer cells. Unlike nontargeted cubosomes, Affimer tagged cubosomes showed preferential accumulation in cancer cells compared to normal cells not only in vitro (2D monolayer cell culture and 3D spheroid models) but also in vivo in colorectal cancer mouse xenografts, while exhibiting low nonspecific absorption and toxicity in other vital organs. Cancerous spheroids had maximum cell death compared to noncancerous cells upon targeted delivery. Xenografts subjected to targeted drug-loaded cubosomes showed a 5–7-fold higher drug accumulation in the tumor tissue compared to the liver, kidneys, and other vital organs, a significant decrease in tumor growth, and an increased survival rate compared to the nontargeted group. This work encompasses the first thorough preclinical investigation of Affimer targeted cubosomes as a cancer therapeutic.

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Section: Materials and Methodsmentioning

confidence: 99%

“… 31 − 34 Affimers, identified from a phage display library, that have specificity toward CEA antigens have been developed 35 and exhibit ease of surface functionalization on molecules of interest. 36 …”

Section: Introductionmentioning

confidence: 99%

Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models

Pramanik

Shamsuddin

et al. 2022

ACS Appl. Mater. Interfaces

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“…In other words, label-free electrochemical immunosensors could directly monitor the binding process of antibody–antigen interaction by amperometric measurements or electrochemical impedance spectroscopy without the involvement of secondary antibodies. 14,18,19 Therefore, more and more attention has been paid to the application of label-free electrochemical immunosensors in the field of tumor marker detection. 20–22 In addition, the detection principle of the electrochemical immunosensor is specific binding of antibodies (Ab) and antigens (Ag), ensuring a fairly high specificity.…”

Section: Introductionmentioning

confidence: 99%

A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

et al. 2021

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“…Tumor biomarkers are related to the occurrence and development of some cancers and can be applied to the early screening, diagnosis, and prognosis of cancer [2,3]. Among them, carcinoembryonic antigen (CEA), a set of glycoproteins highly relevant to cell adhesion, has been considered as a commonly used cancer biomarker in clinical diagnosis, because its overexpression in human blood serum often means the presence or progression of various diseases such as colorectal cancer, pancreatic cancer, and lung carcinoma [4][5][6][7]. Thus, designing facile and accurate methods for ultrasensitive monitoring of CEA is of crucial importance for saving patient lives and helps to stimulate people's demands for medical and health services.…”

Section: Introductionmentioning

confidence: 99%

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Jiang

Xia

Zang

et al. 2021

Sensors

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Recently, electrochemistry- and photoelectrochemistry-based biosensors have been regarded as powerful tools for trace monitoring of carcinoembryonic antigen (CEA) due to the fact of their intrinsic advantages (e.g., high sensitivity, excellent selectivity, small background, and low cost), which play an important role in early cancer screening and diagnosis and benefit people’s increasing demands for medical and health services. Thus, this mini-review will introduce the current trends in electrochemical and photoelectrochemical biosensors for CEA assay and classify them into two main categories according to the interactions between target and biorecognition elements: immunosensors and aptasensors. Some recent illustrative examples are summarized for interested readers, accompanied by simple descriptions of the related signaling strategies, advanced materials, and detection modes. Finally, the development prospects and challenges of future electrochemical and photoelectrochemical biosensors are considered.

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Reagentless Affimer- and antibody-based impedimetric biosensors for CEA-detection using a novel non-conducting polymer

Cited by 37 publications

References 43 publications

Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models

Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models

A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

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