Ready for TDM: Simultaneous quantification of amikacin, vancomycin and creatinine in human plasma employing ultra-performance liquid chromatography-tandem mass spectrometry

Silva, Anne Caroline Cezimbra da; Lizot, Lilian de Lima Feltraco; Bastiani, Marcos Frank; Antunes, Marina Venzon; Brucker, Natália; Linden, Rafael

doi:10.1016/j.clinbiochem.2019.06.011

Cited by 17 publications

(13 citation statements)

References 48 publications

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“…Traditional chromatographic assays for the quantification of any aminoglycoside generally involve a lengthy derivatization process, or using unconventional reagents to resolve particular challenges associated with the chemical properties of aminoglycosides. For example, a recent successful assay opted for HFBA as an ion-pairing reagent to overcome retention time issues with the aminoglycosides [11]. However, HFBA could potentially contaminate the MS and may not be ideal for other assays being run on the same machine, especially those run in the negative mode.…”

Section: Discussionmentioning

confidence: 99%

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A Robust LC–MS/MS Method for Amikacin: Application to Cellular Uptake and Pharmacokinetic Studies

et al. 2020

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Background: Aminoglycosides are last-resort antibiotics for bacterial infections due to concerns of nephrotoxicity. A robust method is needed to correlate the magnitude of drug accumulation in the kidneys and the onset of nephrotoxicity. Materials & methods: A LC–MS/MS assay was developed, circumventing common limitations associated with conventional assays. To demonstrate its applicability, renal cellular uptake and rat pharmacokinetic studies were performed with amikacin. Results: To improve elution, the mobile phases were optimized with 60 mM ammonium hydroxide (pH = 11.2). An extended quantifiable range was achieved with different ionization modes. Kidney cells incubated with escalating amikacin concentrations showed increased uptake. Single-dose pharmacokinetics of amikacin were reasonably characterized. Conclusion: This assay will facilitate future studies on improving amikacin-associated nephrotoxicity.

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Section: Discussionmentioning

confidence: 99%

“…To facilitate this objective, we focused on optimizing the mobile phases. Reagents were selected to reduce potential concerns associated with alternative strategies, such as using ion-pairing reagents (e.g., heptafluorobutyric acid [HFBA]) [11].…”

mentioning

confidence: 99%

A Robust LC–MS/MS Method for Amikacin: Application to Cellular Uptake and Pharmacokinetic Studies

et al. 2020

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“…Interestingly, articles published between 1977 [20] to 2002 [67] were dominated by fluorescence or UV detection methods. More importantly, mass spectrometry was the mostly used detection mode from 2014 to 2020 [34,35,81,[83][84][85], except in one case [82]. This is not surprising as mass spectrometry appears to be an effective detection method [103].…”

Section: A D V a N C E D O N L I N E A R T I C L Ementioning

confidence: 97%

“…Solvents that were used for PPT included acetonitrile for the extraction of netilmicin, amikacin, tobramycin, gentamicin, etimicin, neomycin, and isepamicin [6,15,21,23,32,[37][38][39][40]42,68,[73][74][75][76]78,79,82,86]; trichloroacetic acid for the extraction of geneticin, spectinomycin, tobramycin, gentamicin, kanamycin, hygromycin, apramycin, streptomycin, dihydrostreptomycin, amikacin, neomycin, and paromomycin [29,34,35,57,61,72,81]; methanol for the extraction of geneticin, amikacin, tobramycin, sisomicin, and netilmicin [29,41,48]; perchloric acid for the extraction of kanamycin, dibekacin, arbekacin, and streptomycin [26,33,49,51]; formic acid for the extraction of amikacin [84,85]; and methylene chloride for the extraction of isepamicin [62]. PPT aids in reducing interference during derivatization [7], and this may have accounted for its use by 41 articles.…”

Section: Sample Cleanupmentioning

confidence: 99%

“…Also, mass spectrometry can analyze small sample volumes with high precision, sensitivity, and selectivity [68,81]. In this review, 15 articles reported the use of mass spectrometry as a detector for the bioanalysis of neomycin, streptomycin, dihydrostreptomycin, amikacin, kanamycin, paromomycin, tobramycin, spectinomycin, apramycin, hygromycin, gentamicin, and arbekacin [5,31,[33][34][35]68,69,72,73,75,79,81,[83][84][85]. Interestingly, articles published between 1977 [20] to 2002 [67] were dominated by fluorescence or UV detection methods.…”

Section: A D V a N C E D O N L I N E A R T I C L Ementioning

confidence: 99%

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Bioanalysis of aminoglycosides using high-performance liquid chromatography

et al. 2022

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Aminoglycosides are broad-spectrum antibiotics used in the treatment of gram-negative bacterial infections. Due to their nephrotoxic and ototoxic potential (narrow therapeutic index), the use of aminoglycoside for clinical indications requires monitoring. The objective of this review was to identify relevant literature reporting liquid chromatographic methods for the bioanalysis of aminoglycosides in both preclinical and clinical settings/experiments. Data on liquid chromatographic methods were collected from articles in an online academic database (PubMed, Science Direct, Scopus, and Google Scholar). All 71 articles published from 1977 to 2020 were included in the review. Reversed-phase liquid chromatography was the most used method for the bioanalysis of aminoglycosides. Fluorescence or ultraviolet detection methods were mostly used from 1977 to 2002 (51 articles), while mass spectrometry was predominantly used as a detector from 2003 to 2020 (15 articles). Sixty-seven articles reported calibration ranges, which varied significantly for the various drugs assayed: some in the range of 0.1-0.5 ng/mL and others 1250-200000 ng/mL. Also, 61 articles reported R2 values (0.964-1.0) for almost all analytes under consideration. Sixty-three articles reported percent recoveries mostly between 61.0 % to 114.0 %, with only two articles reporting recoveries of 4.9 % and 36 %. Out of the 71 reviewed articles, 56 reported intermediate precision values ranging between 0.331 % to 19.76 %, which is within the acceptable limit of 20 %. This review will serve as a guide for research and/or routine clinical monitoring of aminoglycosides in biological matrices.

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Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment

Mohamed

2022

J Fluoresc

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Vancomycin hydrochloride (VANH) is a glycopeptide antibiotic commonly employed in the prophylaxis and therapy of various gram-positive bacterial life-threatening infections. Due to the narrow therapeutic window of VANH, its serum levels should be well-monitored to avoid its toxicity and to optimize its therapy. Herein, an innovative silver-nanoparticles enhanced fluorescence technique was designed for VANH rapid analysis in its pharmaceutical formulation and biological fluids. This technique is based on reinforcement of VANH fluorescence intensity with silver-nanoparticles that were synthesized by a redox reaction between VANH and silver nitrate in NaOH alkaline medium using polyvinylpyrrolidone as a stabilizer. The produced silver-nanoparticles were characterized by using UV–visible spectroscopy where they have an intense absorption maximum at 415 nm and transmission electron microscope (TEM) micrograph where they are spherical in shape with smooth surface morphology and size of 10.74 ± 2.44 nm. The fluorescence intensity was measured at 394 nm after excitation at 259 nm. Under optimum conditions, a good linear relationship was accomplished between the VANH concentration and the fluorescence intensity in a range of (1–36) ng/mL with a limit of detection of 0.29 ng/mL. Greenness assessment was performed using two assessment tools namely; eco-scale scoring and green analytical procedure index revealing excellent greenness of the proposed technique. The proposed technique was validated according to the International Conference on Harmonisation (ICH) recommendations and statistically compared with the reported HPLC method revealing no significant difference concerning accuracy and precision at p = 0.05. The proposed technique depended primarily on water as a cheap and eco-friendly solvent.

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Ready for TDM: Simultaneous quantification of amikacin, vancomycin and creatinine in human plasma employing ultra-performance liquid chromatography-tandem mass spectrometry

Cited by 17 publications

References 48 publications

A Robust LC–MS/MS Method for Amikacin: Application to Cellular Uptake and Pharmacokinetic Studies

A Robust LC–MS/MS Method for Amikacin: Application to Cellular Uptake and Pharmacokinetic Studies

Bioanalysis of aminoglycosides using high-performance liquid chromatography

Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment

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