Reading the epigenetic code for exchanging DNA

Biot, Mathilde; Massy, Bernard de

doi:10.7554/elife.61820

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…39,40 ZCWPW1, a reader of histone (H3) modifications, is required to initiate the recombination of genetic information during meiosis. 41 In addition, transcriptomic variations observed in the present study also suggest that the HH diet affects the post-transcriptional regulation of oocyte gene expression, as several differentially expressed genes have been implicated in ribosomal processing like RARS, 42 RIOK1 43 and MRPL55. 44,45 Thus, maternal HH diet altered oocyte transcripts content of genes involved in meiosis and translational control, suggesting difficulties to achieve meiosis and to support the embryo development.…”

Section: Discussionsupporting

confidence: 53%

Alteration of the embryonic microenvironment and sex-specific responses of the preimplantation embryo related to a maternal high-fat diet in the rabbit model

Calderari,

Archilla,

Jouneau

et al. 2023

J Dev Orig Health Dis

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The maternal metabolic environment can be detrimental to the health of the offspring. In a previous work, we showed that maternal high-fat (HH) feeding in rabbit induced sex-dependent metabolic adaptation in the fetus and led to metabolic syndrome in adult offspring. As early development representing a critical window of susceptibility, in the present work we aimed to explore the effects of the HH diet on the oocyte, preimplantation embryo and its microenvironment. In oocytes from females on HH diet, transcriptomic analysis revealed a weak modification in the content of transcripts mainly involved in meiosis and translational control. The effect of maternal HH diet on the embryonic microenvironment was investigated by identifying the metabolite composition of uterine and embryonic fluids collected in vivo by biomicroscopy. Metabolomic analysis revealed differences in the HH uterine fluid surrounding the embryo, with increased pyruvate concentration. Within the blastocoelic fluid, metabolomic profiles showed decreased glucose and alanine concentrations. In addition, the blastocyst transcriptome showed under-expression of genes and pathways involved in lipid, glucose and amino acid transport and metabolism, most pronounced in female embryos. This work demonstrates that the maternal HH diet disrupts the in vivo composition of the embryonic microenvironment, where the presence of nutrients is increased. In contrast to this nutrient-rich environment, the embryo presents a decrease in nutrient sensing and metabolism suggesting a potential protective process. In addition, this work identifies a very early sex-specific response to the maternal HH diet, from the blastocyst stage.

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Section: Discussionsupporting

confidence: 53%

Alteration of the embryonic microenvironment and sex-specific responses of the preimplantation embryo related to a maternal high-fat diet in the rabbit model

Calderari,

Archilla,

Jouneau

et al. 2023

J Dev Orig Health Dis

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“…Recently, three independent groups found that the histone methylation reader ZCWPW1 could specifically recognize dual histone methylation marks (H3K4me3 and H3K36me3) deposited by PRDM9 at recombination hotspots in mice [47][48][49][50]. Much less is known about how ZCWPW1 reads these epigenetic marks and thus participates in the meiotic recombination process to promote DSB repair [51].…”

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confidence: 99%

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

et al. 2022

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Background The PRDM9-dependent histone methylation H3K4me3 and H3K36me3 function in assuring accurate homologous recombination at recombination hotspots in mammals. Beyond histone methylation, H3 lysine 9 acetylation (H3K9ac) is also greatly enriched at recombination hotspots. Previous work has indicated the potential cross-talk between H3K4me3 and H3K9ac at recombination hotspots, but it is still unknown what molecular mechanisms mediate the cross-talk between the two histone modifications at hotspots or how the cross-talk regulates homologous recombination in meiosis. Results Here, we find that the histone methylation reader ZCWPW1 is essential for maintaining H3K9ac by antagonizing HDAC proteins’ deacetylation activity and further promotes chromatin openness at recombination hotspots thus preparing the way for homologous recombination during meiotic double-strand break repair. Interestingly, ectopic expression of the germ-cell-specific protein ZCWPW1 in human somatic cells enhances double-strand break repair via homologous recombination. Conclusions Taken together, our findings provide new insights into how histone modifications and their associated regulatory proteins collectively regulate meiotic homologous recombination.

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Biological Codes: A Field Guide for Code Hunters

Prinz¹

2023

Biol Theory

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Reading the epigenetic code for exchanging DNA

Abstract: Three independent studies show that a protein called ZCWPW1 is able to recognize the histone modifications that initiate the recombination of genetic information during meiosis.

Cited by 4 publications

References 9 publications

Alteration of the embryonic microenvironment and sex-specific responses of the preimplantation embryo related to a maternal high-fat diet in the rabbit model

Alteration of the embryonic microenvironment and sex-specific responses of the preimplantation embryo related to a maternal high-fat diet in the rabbit model

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

Biological Codes: A Field Guide for Code Hunters

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