“…CIE of SV membranes has been observed at inner hair cell synapses from conditional AP-2 KO mice (Jung et al, 2015), cerebellar mossy fiber synapses (at least for low to moderate stimuli; Delvendahl et al, 2016), and in retinal bipolar neurons (Llobet et al, 2011). Moreover, our data are in good agreement with the observed formation of non-coated endocytic intermediates within 4-30 s in microwave-fixed electrically stimulated hippocampal neurons (Schikorski, 2014) and in photoreceptors (Fuchs et al, 2014). Finally, CIE of SVs is consistent with the surprisingly mild phenotypes of genetic KO of clathrin (Sato et al, 2009), AP-2 (Gu et al, 2008;Kononenko et al, 2014), AP180 (Koo et al, 2015), or stonin 2 (Kononenko et al, 2013) on the kinetics of SV retrieval in different organisms and systems.…”