Reactivity of workshop antibodies with a non-ruminant species: Crossreactivity with canine blood leukocytes

Schuberth, Hans‐Joachim; Beer, A E; Rabe, Hardis; Leibold, W.

doi:10.1016/0165-2427(96)05596-1

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…We attempted to eliminate eosinophils in 2 samples of canine bone marrow by adjusting the gate to detect only highly fluorescent cells. Sorted cells represented 5,8,9,10, and 12% of the total number of bone marrow cells for the 5 dogs. Gating (horizonal lines) was used to identify cells with fluorescence intensity greater than that of cells labeled with an isotypematched irrelevant control antibody; thus, gating was used to sort cells on the basis of an increase in fluorescence intensity.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of monoclonal antibodies for identification of subpopulations of myeloid cells in bone marrow obtained from dogs

Weiss¹

2001

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Section: Resultsmentioning

confidence: 99%

Evaluation of monoclonal antibodies for identification of subpopulations of myeloid cells in bone marrow obtained from dogs

Weiss¹

2001

ajvr

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“…The markers cd 4 and cd 8 define subsets of T lymphocytes as in other species, but the expression of cd 4 by granulocytes appears to be unique to the dog (Moore and others 1992); cd 4 is also expressed by dermal dendritic cells in the dog (Moore and others 1996a). The expression of cd 1b is generally restricted to cortical thymocytes and cells of the dendritic cell lineage, and it is only weakly expressed by a subpopulation of circulating monocytes (Schuberth and others 1996). All the histiocytic proliferative diseases express cd 1b (Baines and others 1999, Moore and others 1996a).…”

Section: Discussionmentioning

confidence: 99%

Cutaneous T cell lymphoma mimicking cutaneous histiocytosis: differentiation by flow cytometry

et al. 2000

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A two-year-old, neutered female cross-bred labrador had multiple cutaneous nodules, biopsies of which revealed pathological changes consistent with cutaneous histiocytosis. During a period of one month the dog developed multicentric lymphadenopathy, a retrobulbar mass and masses within the quadriceps and cervical muscles. Fine needle aspiration cytology of the cutaneous nodules and lymph nodes and histological examination of the cutaneous nodules and muscle masses suggested the presence of lymphoblastic lymphoma. A definitive diagnosis of CD8+ T cell lymphoma was achieved by immunophenotyping the tumour cells by flow cytometry.

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“…MHC class II antigens have been reported to be expressed on all canine bone marrow lymphoid cells, therefore, it appears to be a panlymphocyte marker. 7,17 The lymphoid origin of leukemias could be further evaluated by double labeling with CD-14 and Thy-1, or with T-lymphocyte-or B-lymphocyte-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Proliferative Disorders in Canine Bone Marrow by Use of Flow Cytometric Scatter Plots and Monoclonal Antibodies

Weiss

2001

Vet Pathol

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The combination of flow cytometric scatterplot analysis and specific monoclonal antibodies was used to evaluate the lineage of cells from six dogs with proliferative disorders of bone marrow. Scatterplot analysis was used to identify mature and immature myeloid and erythroid cells. The immunophenotype of cells in the immature myeloid gate was determined by labeling cells with four monoclonal antibodies. These results were compared to results of cytologic and cytochemical evaluation. The immunophenotype of a dog with a diagnosis of myelogenous leukemia was a cluster of differentiation-18 (CD-18) positive, CD-14 negative, Thy-1 negative, and a major histocompatibility complex (MHC) class II negative. The immunophenotype of a dog with a diagnosis of myelomonocytic leukemia was CD-18 positive, CD-14 positive, Thy-1 positive, and MHC class II positive. Although this phenotype clearly differentiated myelomonocytic leukemia from myelogenous leukemia, it was similar to the immunophenotype of dogs with a diagnosis of malignant histiocytosis or hemophagocytic syndrome. The immunophenotype of two dogs with myelodysplastic syndrome was CD-18 positive and CD-14 negative. Results for Thy-1 and MHC class II were variable. As additional lineage-specific monoclonal antibodies become available, immunophenotyping should become a valuable tool for determination of the lineage of cells in canine myeloproliferative disorders.

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Reactivity of workshop antibodies with a non-ruminant species: Crossreactivity with canine blood leukocytes

Cited by 13 publications

References 16 publications

Evaluation of monoclonal antibodies for identification of subpopulations of myeloid cells in bone marrow obtained from dogs

Evaluation of monoclonal antibodies for identification of subpopulations of myeloid cells in bone marrow obtained from dogs

Cutaneous T cell lymphoma mimicking cutaneous histiocytosis: differentiation by flow cytometry

Evaluation of Proliferative Disorders in Canine Bone Marrow by Use of Flow Cytometric Scatter Plots and Monoclonal Antibodies

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