Reactivity of pyrrole pigments, XXI: Structure and reactivity of Cu(II) and Zn(II) bilindione chelates

Jaumà, A.; Escuer, Albert; Farrera, Joan‐Anton; Ribó, Josep M.

doi:10.1007/bf00807578

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…Similar results are observed for both miRFPs without His 6 tags (Figure B and Figure S5). In the case of free BV, no change in the spectra was observed for Na + , Ca 2+ , Mg 2+ , Ni 2+ , and Mn 2+ , and BV degradation occurs with Cu 2+ binding (Figure S6) as previously reported for aqueous solutions in air due to a copper-catalyzed oxidation process. ,− Shifts in the BV Soret band and Q band are also observed for Zn 2+ and Co 2+ , and in the case of Zn 2+ , the shift can be reversed with the addition of a chelator.…”

Section: Resultssupporting

confidence: 71%

Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins

Zhao

Zastrow

2022

Biochemistry

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Transition metals such as zinc and copper are essential in numerous life processes, and both deficiency and toxic overload of these metals are associated with various diseases. Fluorescent metal sensors are powerful tools for studying the roles of metal ions in the physiology and pathology of biological systems. Green fluorescent protein (GFP) and its derivatives are highly utilized for protein-based sensor design, but application to anaerobic systems is limited because these proteins require oxygen to become fluorescent. Bacteriophytochrome-based monomeric near-infrared fluorescent proteins (miRFPs) covalently bind a bilin cofactor, which can be added exogenously for anaerobic cells. miRFPs can also have emission wavelengths extending to >700 nm, which is valuable for imaging applications. Here, we evaluated the suitability of miRFP670 and miRFP709 as platforms for single fluorescent protein metal ion sensors. We found that divalent metal ions like Zn 2+ , Co 2+ , Ni 2+ , and Cu 2+ can quench from ∼6−20% (Zn 2+ , Co 2+ , and Ni 2+ ) and up to nearly 90% (Cu 2+ ) of the fluorescence intensity of pure miRFPs and have similar impacts in live Escherichia coli cells expressing miRFPs. The presence of a 6× histidine tag for purification influences metal quenching, but significant Cu 2+ -induced quenching and a picomolar binding affinity are retained in the absence of the His 6 tag in both cuvettes and live bacterial cells. By comparing the Cu 2+ and Cu + -induced quenching results for miRFP670 and miRFP709 and through examining absorption spectra and previously reported crystal structures, we propose a surface metal binding site near the biliverdin IXα chromophore.

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Section: Resultssupporting

confidence: 71%

Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins

Zhao

Zastrow

2022

Biochemistry

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“…11,16 Indeed, related spectral properties were also found in blue transition metal complexes of linear tetrapyrroles obtained via photooxygenolysis of porphyrins. [30][31][32] The spectroscopic behaviour of 1, and its metal complexes, also reminds of that of some articial tripyrrones, 16 as well as of prodigiosenes, natural 'tripyrrolic' alkaloids with interesting antibacterial and antifungal properties, 33 which are also capable of binding a variety of metal ions. 16,33 In methanolic solution, the diamagnetic Cd(II)-and Zn(II)-complexes of 1 exhibited strong red luminescence, whereas the paramagnetic Cu(II)-complex Cu-1 and the Ni(II)-complex Ni-1 showed negligible emission, as expected (see Fig.…”

Section: Discussionmentioning

confidence: 88%

Blue transition metal complexes of a natural bilin-type chlorophyll catabolite

et al. 2014

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“…Roughly similar spectral changes were also reported for transition metal-ions, when giving complexes with other linear tetrapyrroles. [68][69][70] The capacity of PiCC 3 to bind different transition metal ions (such as Zn(II)-, Cd(II)-, Ni(II)-, Cu(II)-, and Pd(II)-ions) reminds of related properties of natural tripyrrolic alkaloids 71 and of artificial tripyrrones. 25 The kinetics of formation of several metal-complexes M-3 of PiCC 3 was analysed qualitatively.…”

Section: Transition Metal Complexes Of Phyllobilinsmentioning

confidence: 99%

Transition metal complexes of phyllobilins – a new realm of bioinorganic chemistry

Kräutler

2015

Dalton Trans.

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Reactivity of pyrrole pigments, XXI: Structure and reactivity of Cu(II) and Zn(II) bilindione chelates

Cited by 9 publications

References 32 publications

Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins

Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins

Blue transition metal complexes of a natural bilin-type chlorophyll catabolite

Transition metal complexes of phyllobilins – a new realm of bioinorganic chemistry

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