Reactivity of Purified Proteins and Polysaccharides from
            <i>Mycobacterium tuberculosis</i>
            in Delayed Skin Test and Cultured Lymphocyte Mitogenesis Assays

Daniel, Thomas M.; Hinz, Carl F.

doi:10.1128/iai.9.1.44-47.1974

Cited by 19 publications

(7 citation statements)

References 13 publications

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“…It seems possible that some mycobacterial polysaccharides may have distinctive antigenic properties that cause them to react differently in different hosts, under differing conditions of immunization, or in different assay systems. This is probably not true for proteincontaining fractions (60,103), and a close correlation between delayed skin test reactions and lymphocyte mitogenesis has been demonstrated with PPD in healthy tuberculin-positive persons (106, 128) and in patients with tuberculosis (94,110). However, these antigenic dichotomies are not necessarily restricted to mycobacterial polysaccharide antigens.…”

mentioning

confidence: 99%

Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Daniel¹,

Janicki²

1978

Microbiological Reviews

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confidence: 99%

Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Daniel¹,

Janicki²

1978

Microbiological Reviews

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“…We have not ruled out the possibility that lipopolysaccharide comigrating with the 35-kDa protein contributes to the observed T-cell response to the MLP fraction. However, other investigators (5,6,17,33), as well as our group (Mohagheghpour, unpublished data), have failed to detect any T-cell responses to mycobacterium-derived lipopolysaccharides, including lipoarabinomannan B of M. leprae. Moreover, previous studies have demonstrated that lipoarabinomannan B nonspecifically inhibits T-cell reactions (6,17).…”

Section: Discussionmentioning

confidence: 76%

Identification of an immunostimulating protein from Mycobacterium leprae

et al. 1990

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Despite the recent identification of a number of Mycobacterium leprae proteins, the major immunogenic determinants of this organism remain obscure. We isolated from M. leprae a potent immunostimulatory preparation, designated the MLP fraction, which contains a major protein of 35 kilodaltons (kDa). This protein was precipitated by monoclonal antibody ML03-A,, which recognizes a 35-kDa protein of M. leprae, and by sera obtained from patients with lepromatous leprosy. Neither sera from healthy controls nor sera from patients with pulmonary tuberculosis recognized the 35-kDa protein, and only one of four serum samples from patients with borderline tuberculoid leprosy reacted with this protein. The MLP fraction stimulated T-cell proliferation in patients with leprosy whose T cells proliferate in response to whole M. leprae cells. Apparently, the T-cell epitope associated with MLP is also expressed on M. tuberculosis and M. bovis BCG, since patients with pulmonary tuberculosis and BCG-vaccinated individuals demonstrated significant responses to the MLP fraction. The 35-kDa M. leprae protein, purified to homogeneity in the laboratory of P. J. Brennan, stimulated T-cell proliferative responses in all MLP-responsive subjects. These findings suggest that the 35-kDa protein present in MLP is an immunostimulatory component of M. leprae. In addition to serving as a useful probe for study of the T-cell anergy associated with lepromatous disease, this protein may ultimately be useful as a component of a vaccine designed to provide protection against infection with M. leprae. * Corresponding author. MATERIALS AND METHODSPatients. Heparinized blood was obtained from 11 patients with LL and 12 patients with borderline tuberculoid leprosy (BTL). The disease stage was classified by T. H. Rea of the Division of Dermatology, University of Southern California, as described by Ridley and Jopling (32). These patients were under treatment with dapsone or a combination of dapsone, rifampin, and clofazimine. None were receiving corticosteroids or thalidomide or had erythema nodosum leprosum when blood samples were taken. All of these patients had been previously studied for the ability of their T cells to proliferate in response to M. leprae in vitro (Mohagheghpour et al., unpublished data). Five of the patients with LL were included in this study because their T-cell responses to M. leprae were normal while the remaining patients with LL were nonresponders to M. leprae. Blood samples were also obtained from 7 patients with pulmonary tuberculosis (PT) from the Curicica Sanitarium, Rio de Janeiro, Brazil; 10 healthy volunteers who had received M. bovis BCG vaccinations; and 8 healthy individuals who had not been vaccinated with BCG.Antigens. Armadillo-derived whole M. leprae cells, lyophilized uninfected armadillo liver, and a 35-kDa M. Ieprae protein purified to homogeneity (single silver-stained band by sodium dodecyl sulfate [SDS]-polyacrylamide gel electrophoresis [PAGE]) were kindly provided by Collins. Recombinant M. leprae proteins ...

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“…Peptides and proteins of intracellular bacteria are considered responsible for the delayed-type hypersensitivity of cell-mediated immunity (DANIEL and HINZ, 1974;JONES and BERMAN, 1975). Although various methods have been used to prepare allergens (LIVE and STUBBS, 1947;OTTOSEN and PLUM, 1949;PARNAS et al, 1966;BHONGBHIBHAT et al.…”

Section: Introductionmentioning

confidence: 99%

Production of Brucella Allergens and Evaluation of their Biological Activity in a Guinea‐pig Bio‐assay

Bercovich

Eger

Dekker

et al. 1995

Journal of Veterinary Medicine, Series B

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Summary A study was conducted to evaluate the biological activity of Brucella allergens extracted with hydrochloride or trichloroacetic acid. Smooth and mucoid Brucella abortus cells and the medium in which brucellae were propagated were used to prepare the allergens. The biological activity of the allergens was estimated in guinea‐pigs sensitized with Brucella abortus strain 544. The guinea‐pigs were intradermally injected with several allergen dilutions. The dilutions were coded and randomized for site of injection so that none of the dilution was injected twice on the same site. Variance analysis using incomplete Latin square was used for the statistical calculation of the results. The calculated biological activity of the allergens was compared with the biological activity of a ‘standard’ allergen that has proved effective in detecting cattle brucellosis. The skin erythema diameter was best when recorded 32 h after allergen injection. Statistical analysis of the skin erythema diameters showed a great variation in biological activity (12–105%) between the allergens. Only the allergen extracted from the medium in which a mucoid Brucella strain was propagated was as potent as the standard. The use of the incomplete Latin square for variance analysis resulted in the estimation of the biological activity of nine batches of allergen in only 27 guinea‐pigs.

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Reactivity of Purified Proteins and Polysaccharides from Mycobacterium tuberculosis in Delayed Skin Test and Cultured Lymphocyte Mitogenesis Assays

Cited by 19 publications

References 13 publications

Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Identification of an immunostimulating protein from Mycobacterium leprae

Production of Brucella Allergens and Evaluation of their Biological Activity in a Guinea‐pig Bio‐assay

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