Reactivity of Isolated Lipopolysaccharides of Enterobacterial R Mutants with Complete or Incomplete Core Structures with Lectins

Ahamed, N.M.; Radziejewska‐Lebrecht, Joanna; Widemann, C.; Mayer, Hubert

doi:10.1016/s0172-5599(80)80053-8

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…Although no knowledge has been available so far on the anomeric linkage of the heptoses in the R. tenue core structure, it seems obvious that the core region is similar or might even be identical to the inner core of enterobacterial core types (Ra, Rl, R2, and R4 [1,4,16,17]). In addition, there is a strong serological cross-reactivity of isolated lipid A of R. tenue with that of Salmonella, although the cross-reactivity is not comlete (7).…”

Section: Discussionmentioning

confidence: 99%

Structure of the heptose region of lipopolysaccharies from Rhodospirillum tenue.

Radziejewska‐Lebrecht¹,

Feige²,

Mayer³

et al. 1981

Journal of Bacteriology

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There is a common structure (core region) in the lipopolysaccharides of Rhodospirillum tenue. It is composed of a branched trisaccharide of L-glycero-D-mannoheptose (and of 2-keto-3-deoxyoctonate), as revealed by methylation analyses of degraded polysaccharides of four different R. tenue strains. The structure is similar or might even be identical to the inner core of enterobacterial O antigens. In addition, each of the four R. tenue lipopolysaccharides contains a strain-specific region that consists of heptose(s) (L-glycero-D-mannoheptose or D-glycero-D-mannoheptose or both) or hexoses. There is a partial substitution of the core region and the strain-specific region by phosphorus, showing microheterogeneity.

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Section: Discussionmentioning

confidence: 99%

Structure of the heptose region of lipopolysaccharies from Rhodospirillum tenue.

Radziejewska‐Lebrecht¹,

Feige²,

Mayer³

et al. 1981

Journal of Bacteriology

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Structural differences in the outer core region of lipopolysaccharides derived from members of the genus Salmonella

Tsang

Schlecht

Mayer

1992

Zentralblatt für Bakteriologie

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Composition and antigenic activity of the oligosaccharide moiety of Haemophilus influenzae type b lipooligosaccharide

Inzana¹,

Seifert²,

Williams³

1985

Infect Immun

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The oligosaccharide moiety of the lipooligosaccharide of Haemophilus influenzae type b strain Eag was isolated from the lipid component by mild acid hydrolysis and purified by gel filtration. Fast atom bombardment-mass spectrometry indicated that the lipid-free oligosaccharide had a basic molecular weight of 1,768; polysaccharides comparable to high-molecular-weight 0 side chains were not found. Glucose, galactose, galactosamine, heptose, 3-deoxy-D-manno-2-octulosonic acid (KDO), ethanolamine, and phosphate were identified in the lipid-free oligosaccharide by colorimetric assays, gas chromatography-mass spectrometry, or an amino acid analyzer. The presence of KDO was not clearly established by a thiobarbituric acid assay or by growth inhibition by a diazaborine derivative thought to block KDO synthesis. However, the semicarbizide assay and gas chromatography-mass spectrometry confirmed the presence of KDO. Lectin precipitation by Eag lipooligosaccharide in gels indicated that P-D-galactose was present and that some of this monosaccharide was a terminal, nonreducing residue linked to N-acetyl-D-galactosamine. The lipid-free oligosaccharide was

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Reactivity of Isolated Lipopolysaccharides of Enterobacterial R Mutants with Complete or Incomplete Core Structures with Lectins

Cited by 4 publications

References 25 publications

Structure of the heptose region of lipopolysaccharies from Rhodospirillum tenue.

Structure of the heptose region of lipopolysaccharies from Rhodospirillum tenue.

Structural differences in the outer core region of lipopolysaccharides derived from members of the genus Salmonella

Composition and antigenic activity of the oligosaccharide moiety of Haemophilus influenzae type b lipooligosaccharide

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