Reactivity of Biologically Important NSAID Compounds with Superoxide (O2.−), nitric oxide (.NO) and Cyclooxygenase Inhibition

Pérez, C.; Sánchez, Juan Pablo; Màrmol, Frederic; Puig-Parellada, P.; Pouplana, Ramón

doi:10.1002/qsar.200630067

Cited by 9 publications

(5 citation statements)

References 38 publications

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“…It is important to emphasize that nitrate may also be formed, thus it should be reduced to nitrite prior to determination. For this purpose, NADH-dependent nitrate reductase was used for elimination of interference of NADH by addition of lactate dehydrogenase and pyruvate (Perez et al 2007). The chromophoric azo derivative formed from nitrite after Griess reaction is then measured spectrophotometrically at 548 nm.…”

Section: Nitric Oxide Radical (No•) Scavenging Assaysmentioning

confidence: 99%

Antioxidants and antioxidant methods: an updated overview

2020

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Antioxidants had a growing interest owing to their protective roles in food and pharmaceutical products against oxidative deterioration and in the body and against oxidative stress-mediated pathological processes. Screening of antioxidant properties of plants and plant-derived compounds requires appropriate methods, which address the mechanism of antioxidant activity and focus on the kinetics of the reactions including the antioxidants. Many studies evaluating the antioxidant activity of various samples of research interest using different methods in food and human health have been conducted. These methods are classified, described, and discussed in this review. Methods based on inhibited autoxidation are the most suited for termination-enhancing antioxidants and for chain-breaking antioxidants, while different specific studies are needed for preventive antioxidants. For this purpose, the most common methods used in vitro determination of antioxidant capacity of food constituents were examined. Also, a selection of chemical testing methods was critically reviewed and highlighted. In addition, their advantages, disadvantages, limitations and usefulness were discussed and investigated for pure molecules and raw extracts. The effect and influence of the reaction medium on the performance of antioxidants are also addressed. Hence, this overview provides a basis and rationale for developing standardized antioxidant methods for the food, nutraceuticals, and dietary supplement industries. In addition, the most important advantages and shortcomings of each method were detected and highlighted. The chemical principles of these methods are outlined and critically discussed. The chemical principles of methods of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS •+ ) scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging, Fe 3+ -Fe 2+ transformation assay, ferric reducing antioxidant power (FRAP) assay, cupric ions (Cu 2+ ) reducing power assay (Cuprac), Folin-Ciocalteu reducing capacity (FCR assay), peroxyl radical (ROO•), superoxide radical anion (O 2•− ), hydrogen peroxide (H 2 O 2 ) scavenging assay, hydroxyl radical (OH•) scavenging assay, singlet oxygen ( 1 O 2 ) quenching assay, nitric oxide radical (NO•) scavenging assay and chemiluminescence assay are outlined and critically discussed. Also, the general antioxidant aspects of main food components were discussed by a number of methods, which are currently used for the detection of antioxidant properties of food components. This review consists of two main sections. The first section is devoted to the main components in the food and pharmaceutical applications. The second general section comprises some definitions of the main antioxidant methods commonly used for the determination of the antioxidant activity of components. In addition, some chemical, mechanistic and kinetic basis, and technical details of the used methods are given.

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Section: Nitric Oxide Radical (No•) Scavenging Assaysmentioning

confidence: 99%

Antioxidants and antioxidant methods: an updated overview

2020

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“…It is important to emphasize that nitrate may also be formed, thus it should be reduced to nitrite prior to determination. For this, Perez et al used NADH-dependent nitrate reductase, with elimination of interference of NADH by addition of lactate dehydrogenase and pyruvate [154]. The chromophoric azo derivative formed from nitrite after Griess reaction is then measured spectrophotometrically at 540 nm.…”

Section: Nitric Oxide Radical (No • ) Scavenging Capacity Assaysmentioning

confidence: 99%

Methodological aspects about in vitro evaluation of antioxidant properties

Magalhães

Segundo

Reis

et al. 2008

Analytica Chimica Acta

592

404

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“…For this situation, the nitric oxide staying after interaction with the sample is measured as nitrite. It is significant to confirm that nitrate can be formed, hence it ought to be diminished to nitrite before determination [ 35 ]. The derivative of azo chromophor was formed from nitrite after Griess reaction determined spectrophoto-metrically at 540 nm.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activities of 4-Methylumbelliferone Derivatives

et al. 2016

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The synthesis of derivatives of 4-Methylumbelliferone (4-MUs), which are structurally interesting antioxidants, was performed in this study. The modification of 4-Methylumbelliferone (4-MU) by different reaction steps was performed to yield the target compounds, the 4-MUs. The 4-MUs were characterized by different spectroscopic techniques (Fourier transform infrared; FT-IR and Nuclear magnetic resonance; NMR) and micro-elemental analysis (CHNS). The in vitro antioxidant activity of the 4-MUs was evaluated in terms of their free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH), Nitric oxide radical scavenging activity assay, chelating activity and their (FRAP) ferric-reducing antioxidant power, which were compared with a standard antioxidant. Our results reveal that the 4-MUs exhibit excellent radical scavenging activities. The antioxidant mechanisms of the 4-MUs were also studied. Density Function Theory (DFT)-based quantum chemical studies were performed with the basis set at 3-21G. Molecular models of the synthesized compounds were studied to understand the antioxidant activity. The electron levels, namely HOMO (highest occupied molecular orbital) and LUMO (lowest unoccupied molecular orbital), for these synthesized antioxidants were also studied.

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Reactivity of Biologically Important NSAID Compounds with Superoxide (O₂^.−), nitric oxide (^.NO) and Cyclooxygenase Inhibition

Cited by 9 publications

References 38 publications

Antioxidants and antioxidant methods: an updated overview

Antioxidants and antioxidant methods: an updated overview

Methodological aspects about in vitro evaluation of antioxidant properties

Antioxidant Activities of 4-Methylumbelliferone Derivatives

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