Reactivity and stability of plasma-generated oxygen and nitrogen species in buffered water solution: a computational study

Heirman, Pepijn; Boxem, Wilma Van; Bogaerts, Annemie

doi:10.1039/c9cp00647h

Cited by 59 publications

(64 citation statements)

References 45 publications

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“…This addresses one of the main reasons for the scepticism towards plasma medicine. Direct plasma treatment provides targeted delivery of highly short-lived RONS during treatment, while plasma-treated media generates persistent and semi-persistent molecular and ionic compounds, including e.g., H 2 O 2 , nitrous and peroxynitrous acid [13,55]. These compounds are commercially available, and their use technically does not require the employment of cold plasma.…”

Section: Identifying Optimal Parameters For Anti-cancer Treatmentsmentioning

confidence: 99%

Applications of the COST Plasma Jet: More than a Reference Standard

Gorbanev

Golda

Gathen

et al. 2019

Plasma

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The rapid advances in the field of cold plasma research led to the development of many plasma jets for various purposes. The COST plasma jet was created to set a comparison standard between different groups in Europe and the world. Its physical and chemical properties are well studied, and diagnostics procedures are developed and benchmarked using this jet. In recent years, it has been used for various research purposes. Here, we present a brief overview of the reported applications of the COST plasma jet. Additionally, we discuss the chemistry of the plasma-liquid systems with this plasma jet, and the properties that make it an indispensable system for plasma research.

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Section: Identifying Optimal Parameters For Anti-cancer Treatmentsmentioning

confidence: 99%

Applications of the COST Plasma Jet: More than a Reference Standard

Gorbanev

Golda

Gathen

et al. 2019

Plasma

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“…The downward‐directed He jet can deform the surface of the liquid and induce circulation in the liquid . We have chosen a more simplified model with a nondeforming surface.…”

Section: Description Of the Modelmentioning

confidence: 99%

“…The downward-directed He jet can deform the surface of the liquid and induce circulation in the liquid. [40] We have chosen a more simplified model with a nondeforming surface. We expect that for the high-rimmed cases, there would be little effect on the gas flow patterns due to the curvature of the liquid as recirculation is dominated by the vertical walls.…”

Section: Description Of the Modelmentioning

confidence: 99%

Helium plasma jet interactions with water in well plates

Mohades

Lietz

Kruszelnicki

et al. 2019

Plasma Processes & Polymers

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Plasma activation of liquids or cell cultures is a method for investigating the consequences of plasma produced reactive oxygen and nitrogen species (RONS) on living systems. The reproducible transfer of RONS, ions, electrons, and photons to the liquid is critical for determining reaction mechanisms and biological outcomes, and depends strongly on system parameters. A common in vitro method of plasma treatment of cells is a plasma jet directed into a well plate filled (or partially filled) with a liquid cell growth media. This method of treatment intrinsically has several environmental and geometrical factors that could lead to variability in the activation of the media. Uncontrolled or unreported geometrical and environmental factors that affect this transfer can, therefore, influence the reproducibility of measurements. In this paper, results from a numerical modeling investigation of a pulsed helium plasma jet interacting with water in a well plate are discussed while varying the height of the well‐plate rim. The height of the rim changes gas flow patterns, the ratio of He‐to‐air, and the water vapor content in the gas layer above the liquid. With a low rim, gas flowing from the jet stagnates on‐axis and flows radially outward with few vortices that recirculate reactants. With high rims, the gas flow is dominated by vortices and recirculation. With a low rim, the ionization wave (IW) from the jet strikes the liquid and proceeds as a surface IW across the water. With higher rims, the densities of helium and water vapor are higher above the liquid, which results in a volumetric propagation of the IW, initially producing higher densities of H2, HO2, OH, and H2O2. The end result is that for otherwise identical conditions, the densities of solvated H2O2aq and select reactive nitrogen species increase with rim height due to the vortices that recirculate reactants.

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“…It is understandable that non-cancerous cells normally require more advanced cell culture media with additional organic compounds compared to cancer cells, but the different media have disparate buffering and antioxidant capacities [31]. In fact, the stability of RONS in different liquids has been thoroughly investigated [32,33] and Yan et. al showed that the presence of cysteine and methionine can significantly degrade CAP-generated RONS [31].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Cell Type and Culture Medium on Determining Cancer Selectivity of Cold Atmospheric Plasma Treatment

Biscop

Lin

Boxem

et al. 2019

Cancers

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Increasing the selectivity of cancer treatments is attractive, as it has the potential to reduce side-effects of therapy. Cold atmospheric plasma (CAP) is a novel cancer treatment that disrupts the intracellular oxidative balance. Several reports claim CAP treatment to be selective, but retrospective analysis of these studies revealed discrepancies in several biological factors and culturing methods. Before CAP can be conclusively stated as a selective cancer treatment, the importance of these factors must be investigated. In this study, we evaluated the influence of the cell type, cancer type, and cell culture medium on direct and indirect CAP treatment. Comparison of cancerous cells with their non-cancerous counterparts was performed under standardized conditions to determine selectivity of treatment. Analysis of seven human cell lines (cancerous: A549, U87, A375, and Malme-3M; non-cancerous: BEAS-2B, HA, and HEMa) and five different cell culture media (DMEM, RPMI1640, AM, BEGM, and DCBM) revealed that the tested parameters strongly influence indirect CAP treatment, while direct treatment was less affected. Taken together, the results of our study demonstrate that cell type, cancer type, and culturing medium must be taken into account before selectivity of CAP treatment can be claimed and overlooking these parameters can easily result in inaccurate conclusions of selectivity.

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Reactivity and stability of plasma-generated oxygen and nitrogen species in buffered water solution: a computational study

Abstract: Plasma-treated liquids have great potential for biomedical applications.

Cited by 59 publications

References 45 publications

Applications of the COST Plasma Jet: More than a Reference Standard

Applications of the COST Plasma Jet: More than a Reference Standard

Helium plasma jet interactions with water in well plates

The Influence of Cell Type and Culture Medium on Determining Cancer Selectivity of Cold Atmospheric Plasma Treatment

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