Reactivity and DNA Damage by Independently Generated 2′-Deoxycytidin-<i>N</i>4-yl Radical

Peng, Haihui; Jie, Jialong; Mortimer, Ifor P.; Ma, Zehan; Su, Hongmei; Greenberg, Marc M.

doi:10.1021/jacs.1c06425

Cited by 11 publications

(22 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 ) [ 86 ]. In the DNA, oxidative stress can induce several types of damage, including single- and double-strand brakes, DNA-protein crosslinks and base and sugar oxidation products, such as guanine oxidized species (GOS) [ [86] , [87] , [88] ]. A study assessed the presence of GOS in the serum of critical COVID-19 patients from intensive care units [ 56 ].…”

Section: Potential Indirect Mechanisms Of Sars-cov-2 Induced Dna Damage: Aberrant Inflammation Immune Response and Oxidative Damagementioning

confidence: 99%

The potential role of COVID-19 in the induction of DNA damage

Pánico

Ostrosky‐Wegman

Salazar

2022

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

The coronavirus disease-2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is challenging global health and economic systems. In some individuals, COVID-19 can cause a wide array of symptoms, affecting several organs, such as the lungs, heart, bowels, kidneys and brain, causing multiorgan failure, sepsis and death. These effects are related in part to direct viral infection of these organs, immunological deregulation, a hypercoagulatory state and the potential for development of cytokine storm syndrome. Since the appearance of COVID-19 is recent, the long-term effects on the health of recovered patients remain unknown. In this review, we focused on current evidence of the mechanisms of DNA damage mediated by coronaviruses. Data supports that these viruses can induce DNA damage, genomic instability, and cell cycle deregulation during their replication in mammalian cells. Since the induction of DNA damage and aberrant DNA repair mechanisms are related to the development of chronic diseases such as cancer, diabetes, neurodegenerative disorders, and atherosclerosis, it will be important to address similar effects and outcomes in recovered COVID-19 patients.

show abstract

Section: Potential Indirect Mechanisms Of Sars-cov-2 Induced Dna Damage: Aberrant Inflammation Immune Response and Oxidative Damagementioning

confidence: 99%

The potential role of COVID-19 in the induction of DNA damage

Pánico

Ostrosky‐Wegman

Salazar

2022

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

show abstract

“…Chronic inflammation increases the formation of reactive oxygen species (Bartsch and Nair 2004). Oxidative stress can induce various types of damage, including in the double-strand DNA, DNA-protein crosslinks, and oxidation products of sugar groups (Peng et al 2021). Studies have shown that oxidative DNA damage contributes to the development of neurological diseases and several types of cancer (37,38).…”

Section: Discussionmentioning

confidence: 99%

Investigation of inflammation, oxidative stress, and DNA damage in COVID-19 patients

Tepebaşı

İlhan

Temel

et al. 2023

Cell Stress and Chaperones

View full text Add to dashboard Cite

COVID-19 disease, which spreads worldwide, is a disease characterized by widespread inflammation and affects many organs, especially the lungs. The resulting inflammation can lead to reactive oxygen radicals, leading to oxidative DNA damage. The pneumonia severity of 95 hospitalized patients with positive RT-PCR test was determined and divided into three groups: mild, moderate, and severe/critical. Inflammation markers (neutrophil-lymphocyte ratio, serum reactive protein, procalcitonin, etc.) were determined, and IL-10 and IFN-γ measurements were analyzed using the enzyme-linked immunosorbent assay method. In evaluating oxidative damage, total thiol, native thiol, disulfide, and ischemia-modified albumin (IMA) levels were determined by measuring spectrophotometrically. The comet assay method's percentage of tail DNA obtained was used to determine oxidative DNA damage. As a result, when the mild and severe/critical groups were compared, we found that total thiol, native thiol, and disulfide levels decreased significantly in the severe/critical group due to the increase in inflammation markers and cytokine levels (p < 0.05). We could not detect any significance in IMA levels between the groups (p > 0.05). At the same time, we determined an increase in the tail DNA percent level, that is, DNA damage, due to the increased oxidative effect. As a result, we determined that inflammation and oxidative stress increased in patients with severe pneumonia, and there was DNA damage in these patients.

show abstract

“…Chronic in ammation increases the formation of reactive oxygen species [36]. Oxidative stress can induce various types of damage, including in the DNA double-strand, DNA-protein crosslinks, and oxidation products of sugar groups [37]. Studies have shown that oxidative DNA damage contributes to the development of neurological diseases and several types of cancer (37,38).…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress can induce various types of damage, including in the DNA double-strand, DNA-protein crosslinks, and oxidation products of sugar groups [37]. Studies have shown that oxidative DNA damage contributes to the development of neurological diseases and several types of cancer (37,38). DNA damage caused by oxidative stress can be determined by Tail DNA percent levels [40].…”

Section: Discussionmentioning

confidence: 99%

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

Tepebaşı

İlhan

Temal

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: COVID-19 disease, which spreads all over the world, is a disease characterized by widespread inflammation and affects many organs, especially the lungs. The resulting inflammation can lead to the generation of reactive oxygen radicals, leading to oxidative DNA damage.Methods and Results: Pneumonia severity of a total of 95 hospitalized patients with positive RT-PCR test was determined and divided into three groups mild, moderate, and severe/critical. Inflammation markers (Neutrophil-lymphocyte ratio, serum reactive protein, procalcitonin, etc.) were determined, and IL-10 and IF γ measurements were analyzed using the enzyme-linked immunosorbent assay method. In the evaluation of oxidative damage, total thiol, native thiol, disulfide, and IMA levels were determined by measuring spectrophotometrically. The percentage of tail DNA obtained by the comet assay method was used to determine oxidative DNA damage. As a result, when mild and severe/critical groups were compared, we found that total thiol, native thiol, and disulfide levels decreased significantly in the severe/critical group due to the increase in inflammation markers and cytokine levels (p <0.05). We could not detect any significance in IMA levels between the groups (p> 0.05). At the same time, we determined that there was an increase in the tail DNA percent level, that is, DNA damage, due to the increased oxidative effect.Conclusion: As a result, we found that increased inflammation and reactive oxygen species due to the increase in pneumonia increased in those with severe and severe pneumonia, which in turn damaged DNA. We think that it would be useful to follow up on the DNA damage in these patients in terms of neurodegenerative diseases and cancer development.

show abstract

Reactivity and DNA Damage by Independently Generated 2′-Deoxycytidin-N4-yl Radical

Cited by 11 publications

References 62 publications

The potential role of COVID-19 in the induction of DNA damage

The potential role of COVID-19 in the induction of DNA damage

Investigation of inflammation, oxidative stress, and DNA damage in COVID-19 patients

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

Contact Info

Product

Resources

About