Reactivities of organic phase biosensors. 2. The amperometric behaviour of horseradish peroxidase immobilised on a platinum electrode modified with an electrosynthetic polyaniline film

Iwuoha, Emmanuel I.; Villaverde, David Saenz de; Gaspar, Ignacio De; Smyth, Malcolm R.; Pingarrón, José M.

doi:10.1016/s0956-5663(97)00042-0

Cited by 110 publications

(70 citation statements)

References 39 publications

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“…The reduced PEDT was necessary as an electron transfer mediator to complete the electron transfer cycle as described in Ref. 15. Hydrogen peroxide ͑5 mM͒ was added once the current had reached steady state.…”

Section: Resultsmentioning

confidence: 99%

A Simple Means to Immobilize Enzyme into Conducting Polymers via Entrapment

Winther‐Jensen

Lynam

Ngamna

et al. 2006

Electrochem. Solid-State Lett.

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Section: Resultsmentioning

confidence: 99%

A Simple Means to Immobilize Enzyme into Conducting Polymers via Entrapment

Winther‐Jensen

Lynam

Ngamna

et al. 2006

Electrochem. Solid-State Lett.

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“…5 (D)). The value of D was affected by the concentration of the electrolyte used in this study and is generally subject to uncertainties in volume and homogeneity of the film [38][39].…”

Section: Fig 3 Sem Image Of Feco Nanoparticlesmentioning

confidence: 99%

Electrokinetic and Impedimetric Dynamics of FeCo-Nanoparticles on Glassy Carbon Electrode

Ikpo

Njomo

Ozoemena³

et al. 2013

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Abstract. The electrochemical dynamics of a film of FeCo nanoparticles were studied on a glassy carbon electrode (GCE). The film was found to be electroactive in 1 M LiClO 4 containing 1:1 v/v ethylene carbonate -dimethyl carbonate electrolyte system. Cyclic voltammetric experiments revealed a diffusion-controlled electron transfer process on the GCE/FeCo electrode surface. Further interrogation on the electrochemical properties of the FeCo nanoelectrode in an oxygen saturated 1 M LiClO 4 containing 1:1 v/v ethylene-carbonate-dimethyl carbonate revealed that the nanoelectrode showed good response towards the electro-catalytic reduction of molecular oxygen with a Tafel slope of about 120 mV which is close to the theoretical 118 mV for a single electron transfer process in the rate limiting step; and a transfer coefficient (α) of 0.49. The heterogeneous rate constant of

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“…Drop-coating of the nanoPANI/DBSA dispersion onto electrodes was carried out as an alternative deposition method to the more commonly utilised electropolymerisation method [2][3][4][7][8][9][10][11][12]. Electropolymerisation is not a technique applicable to cost-effective mass production of biosensors.…”

Section: Drop-coating Of Nanopani/dbsa Onto Screenprinted Electrodesmentioning

confidence: 99%

“…In many instances, modification of electrodes with polyaniline has been applied in biosensing applications [2][3][4][5][6][7][8][9][10][11][12], where polyaniline can act as an effective non-diffusional mediating species coupling electrons directly from the enzyme redox site to the electrode. This allows for very effective direct electrical communication between the biomolecule and the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

“…This allows for very effective direct electrical communication between the biomolecule and the electrode surface. This format has been described for many enzymes including glucose oxidase [6][7][8], horseradish peroxidase (HRP) [9], uricase [10], ascorbate oxidase [11] and sarcosine oxidase [12]. Polyaniline (PANI) exhibits good environmental stability, and its electrical properties can be modified by the oxidation state of the main chain and degree of protonation for different applications including the aforementioned biosensors, microporous electrically conducting materials, anti-corrosion protection of metals, chemical sensing, supporting material for catalysts, etc.…”

Section: Introductionmentioning

confidence: 99%

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Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles

Morrin

Wilbeer

Ngamna

et al. 2005

Electrochemistry Communications

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This work investigates polyaniline (PANI) nanoparticles, (synthesised using dodecylbenzenesulphonic acid (DBSA) as a dopant), as a novel, highly processable, non-diffusional mediating species in an enzyme biosensing application. These nanoparticles are readily dispersed in aqueous media which helps overcome some of the processability issues traditionally associated with polyaniline. Modification of screen-printed electrodes was readily achieved with these aqueous nanoparticle dispersions, where the nanoparticles were simply cast by a drop-coating method onto the surface. After suitable pH adjustment, it was shown that horseradish peroxidase (HRP) enzyme could be added to the dispersion, and cast simultaneously with the conducting polyaniline. This effective fabrication method involves no electrochemical steps, and as such is easily amenable to mass production. The feasibility of casting enzyme with polyaniline nanoparticles is demonstrated in this short communication. More accurate deposition of protein-containing inks onto screen-printed carbon working electrodes could in the future transfer the drop-coating protocol from manual deposition to largescale production by mechanical methods such as ink-jet printing.

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Reactivities of organic phase biosensors. 2. The amperometric behaviour of horseradish peroxidase immobilised on a platinum electrode modified with an electrosynthetic polyaniline film

Cited by 110 publications

References 39 publications

A Simple Means to Immobilize Enzyme into Conducting Polymers via Entrapment

A Simple Means to Immobilize Enzyme into Conducting Polymers via Entrapment

Electrokinetic and Impedimetric Dynamics of FeCo-Nanoparticles on Glassy Carbon Electrode

Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles

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