Reactive oxygen species (ROS) production by amoebocytes of Asterias rubens (Echinodermata)

Coteur, Geoffroy; Warnau, Michel; Jangoux, Michel; Dúbois, Philippe

doi:10.1006/fsim.2001.0366

Cited by 87 publications

(47 citation statements)

References 28 publications

(39 reference statements)

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“…Similarly, in some cnidarians, including gorgonians, scleractinian corals, and sea anemones, amoebocytes are recruited from the mesoglea to the wound site, and these cells engage in phagocytosis (Hutton and Smith, 1996;Meszaros and Bigger, 1999;Olano and Bigger, 2000;Henry and Hart, 2005;Mydlarz et al, 2006). These amoebocytes that migrate to the wound site also produce ROS (Hutton and Smith, 1996), as occurs in the circulating amoebocytes of other invertebrates (Nakamura et al, 1985;Coteur et al, 2002), suggesting that these cells are playing a role in an inflammatory response.…”

Section: Wound Healing In Cnidariansmentioning

confidence: 99%

Genomic Survey of Candidate Stress-Response Genes in the Estuarine AnemoneNematostella vectensis

Reitzel

Sullivan

Traylor-Knowles

et al. 2008

The Biological Bulletin

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Abstract. Salt marshes are challenging habitats due to natural variability in key environmental parameters including temperature, salinity, ultraviolet light, oxygen, sulfides, and reactive oxygen species. Compounding this natural variation, salt marshes are often heavily impacted by anthropogenic insults including eutrophication, toxic contamination, and coastal development that alter tidal and freshwater inputs. Commensurate with this environmental variability, estuarine animals generally exhibit broader physiological tolerances than freshwater, marine, or terrestrial species. One factor that determines an organism's physiological tolerance is its ability to upregulate "stressresponse genes" in reaction to particular stressors. Comparative studies on diverse organisms have identified a number of evolutionarily conserved genes involved in responding to abiotic and biotic stressors. We used homologybased scans to survey the sequenced genome of Nematostella vectensis, the starlet sea anemone, an estuarine specialist, to identify genes involved in the response to three kinds of insult-physiochemical insults, pathogens, and injury. Many components of the stress-response networks identified in triploblastic animals have clear orthologs in the sea anemone, meaning that they must predate the cnidariantriploblast split (e.g., xenobiotic receptors, biotransformative genes, ATP-dependent transporters, and genes involved in responding to reactive oxygen species, toxic metals, osmotic shock, thermal stress, pathogen exposure, and wounding). However, in some instances, stress-response genes known from triploblasts appear to be absent from the Nematostella genome (e.g., many metal-complexing genes). This is the first comprehensive examination of the genomic stress-response repertoire of an estuarine animal and a member of the phylum Cnidaria. The molecular markers of stress response identified in Nematostella may prove useful in monitoring estuary health and evaluating coastal conservation efforts. These data may also inform conservation efforts on other cnidarians, such as the reef-building corals.

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Section: Wound Healing In Cnidariansmentioning

confidence: 99%

Genomic Survey of Candidate Stress-Response Genes in the Estuarine AnemoneNematostella vectensis

Reitzel

Sullivan

Traylor-Knowles

et al. 2008

The Biological Bulletin

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“…The production of extracellular reactive oxygen species (ROS) was determined using the peroxidase luminolenhanced chemiluminescence (PLCL) assay described by Coteur et al (2002) as modified by Ripley et al (2008). In short, 1 9 10 6 leukocytes suspended in AM were added to the wells of a white 96-well plate, incubated for adherence as above, washed and subsequently incubated in CM to allow cells to reach a resting state.…”

Section: Respiratory Burstmentioning

confidence: 99%

Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River

et al. 2011

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The use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. We examined the health of the Ashtabula River using histologic, immunologic, and endocrine biomarkers in brown bullhead (BB; Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) and compared fish collected from a reference site (Conneaut Creek). Seasonal analysis was necessary to distinguish differences in fish between the two rivers. Overall BB from the Ashtabula River had a lower condition factor and significantly more macrophage aggregates than those from the reference site. Reduced bactericidal and cytotoxic-cell activity was observed in anterior kidney leukocytes from both BB and largemouth bass from the Ashtabula River. Lower plasma thyroxine and triiodo-L-thyronine in both species in the Ashtabula River indicated disruption of the thyroid axis. Differences in physiological biomarker responses were supported by body burden chemical concentrations when data were analyzed on a seasonal basis. The use of two fish species added a level of rigor that demonstrated biological effects were not exclusive to a single species. The results provide strong evidence that contaminants have affected fish in the Ashtabula River, a Great Lakes Area of Concern, and provide a baseline by which to evaluate remediation activities.

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“…Amoebocyte ROS production was measured by the peroxidase luminol-enhanced chemiluminescence (PLCL), method developed by Coteur et al (2002Coteur et al ( , 2004Coteur et al ( , 2005. Measurements were normalized using the actual amoebocyte concentration in each sample, and expressed as the sum of all 10-min interval measurements (in Relative Light Units RLU, an internal scale of the instrument) for 10 6 cells ml -1 (total chemiluminescence) of resting or stimulated amoebocytes.…”

Section: Immune Response Measurementmentioning

confidence: 99%

Effects of the Erika Oil Spill on the Common Starfish Asterias rubens, Evaluated by Field and Laboratory Studies

Joly-Turquin

Dúbois

Coteur

et al. 2008

Arch Environ Contam Toxicol

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Reactive oxygen species (ROS) production by amoebocytes of Asterias rubens (Echinodermata)

Cited by 87 publications

References 28 publications

Genomic Survey of Candidate Stress-Response Genes in the Estuarine AnemoneNematostella vectensis

Genomic Survey of Candidate Stress-Response Genes in the Estuarine AnemoneNematostella vectensis

Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River

Effects of the Erika Oil Spill on the Common Starfish Asterias rubens, Evaluated by Field and Laboratory Studies

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