Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation

Morado, Sergio Adrián; Cetica, Pablo Daniel; Beconi, M.T.; Thompson, Jeremy G.; Dalvit, Gabriel Carlos

doi:10.1530/rep-13-0017

Cited by 28 publications

(19 citation statements)

References 45 publications

(27 reference statements)

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“…However, a significant number of patients with normal sperm parameters still undergo difficulty in achieving successful pregnancy [ 13 ]. Several new diagnostic biomarkers for semen quality have been investigated recently including PLCζ, oxidative stress and DFI [ 14 ]. Here, we first demonstrated that sperm PLCζ immunoreactivity has a negative correlation with a oxidation marker, 8-OHdG.…”

Section: Discussionmentioning

confidence: 99%

Relationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm

Park

Kim

et al. 2015

Obstet Gynecol Sci

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ObjectiveThe study aimed to evaluate the feasibility and reproducibility of measuring phospholipase C zeta (PLCζ) using immunostaining in human sperm and to investigate the relationship between PLCζ immunoreactivity and DNA fragmentation and oxidation in human sperm.MethodsSemen samples were obtained from participants (n=44) and processed by the conventional swim-up method. Sperm concentration, motility, normal form by strict morphology, DNA fragmentation index assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling method and immunofluorescent expression for 8-hydroxy-2'-deoxyguanosine (8-OHdG) and PLCζ were assessed.ResultsWhen duplicate PLCζ tests were performed on two sperm samples from each of the 44 participants, similar results were obtained (74.1±9.4% vs. 75.4±9.7%). Two measurements of PLCζ were found to be highly correlated with each other (r=0.759, P<0.001). Immunoreactivity of PLCζ was not associated with donor's age, sperm concentration, motility, and the percentage of normal form as well as DNA fragmentation index. However, immunoreactivity of PLCζ showed a significant negative relationship with 8-OHdG immunoreactivity (r=-0.404, P=0.009).ConclusionMeasurement of PLCζ by immunostaining is feasible and reproducible. Lower expression of PLCζ in human sperm may be associated with higher sperm DNA oxidation status.

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Section: Discussionmentioning

confidence: 99%

Relationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm

Park

Kim

et al. 2015

Obstet Gynecol Sci

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“…[4] Hydrogen peroxide (H 2 O 2 ) is an important ROS in reproductive health, and its detection is vital for monitoring embryo health and the diagnosis of male infertility. H 2 O 2 is typically detected in cells using a compound (fluorophore) that fluoresces on its reaction with ROS.…”

Section: Introductionmentioning

confidence: 99%

Localised hydrogen peroxide sensing for reproductive health

et al. 2015

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Authors, or their employers in the case of works made for hire, retain the following rights: 1. All proprietary rights other than copyright, including patent rights. 2. The right to make and distribute copies of the Paper for internal purposes. 3. The right to use the material for lecture or classroom purposes. 4. The right to prepare derivative publications based on the Paper, including books or book chapters, journal papers, and magazine articles, provided that publication of a derivative work occurs subsequent to the official date of publication by SPIE.5. The right to post an author-prepared version or an official version (preferred version) of the published paper on an internal or external server controlled exclusively by the author/employer, provided that (a) such posting is noncommercial in nature and the paper is made available to users without charge; (b) a copyright notice and full citation appear with the paper, and (c) a link to SPIE's official online version of the abstract is provided using the DOI (Document Object Identifier) link. Citation format:Author(s), "Paper Title," Publication Title, Editors, Volume (Issue) Number, Article (or Page) Number, (Year). Copyright notice format:Copyright XXXX (year) Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ABSTRACTThe production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H 2 O 2 ) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H 2 O 2 production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H 2 O 2 that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H 2 O 2 due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H 2 O 2 . Using this method, biologically relevant concentrations of H 2 O 2 ...

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“…It has been suggested that certain amount of ROS is essential for the successful fertilization process [ 35 ]. In case of sperm-activated oocytes, ROS peaks are associated with fertilization events such as sperm penetration and sperm head decondensation [ 36 ]. However, our data suggest that increase in ROS level is independent of sperm deposition but may be related to mechanical stress induced during mock injection process since both in vivo matured and IVM oocytes subjected to mock injection had significantly higher level of ROS compared to standard and ICSI controls.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Matured Oocytes Are More Susceptible than In Vivo Matured Oocytes to Mock ICSI Induced Functional and Genetic Changes

et al. 2015

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BackgroundConcerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome.ObjectiveThe present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes.MethodsReactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI.ResultsA significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001).ConclusionThe data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.

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Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation

Cited by 28 publications

References 45 publications

Relationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm

Relationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm

Localised hydrogen peroxide sensing for reproductive health

In Vitro Matured Oocytes Are More Susceptible than In Vivo Matured Oocytes to Mock ICSI Induced Functional and Genetic Changes

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