Reactive Oxygen Species Participate in Peroxynitrite-Induced Apoptosis in HL-60 Cells

Lin, King-Teh; Xue, Jiyan; Sun, Frank F.; Wong, Patrick Y.-K.

doi:10.1006/bbrc.1996.5897

Cited by 106 publications

(50 citation statements)

References 24 publications

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“…The cytotoxic effects of Cd are related to its accumulation in the nucleus by specific metallothioneins and by a strong inhibition effect on superoxide dismutase, peroxidase and catalase, which are major scavenger enzymes of intracellular superoxides. Oxidative stress has been shown to be one of the predominant factors related to apoptosis through peroxinitrite formation (Lin et al, 1997;Stoian et al, 1996;Wolfe et al, 1994). Copper may induce apoptosis through pore formation in the mitochondrial membrane.…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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Section: Discussionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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show abstract

“…56,62 The interaction of NO and superoxide anion and its subsequent production of peroxynitrite has been shown to cause alterations in cavernosal smooth muscle relaxation in both humans and in rats. 35,37,39 This situation presents a unique situation in that iNOS expression and subsequent high output NO production may be beneficial in suppressing collagen accumulation in Peyronie's disease but at the same time causes deleterious consequences in the corpus cavernosum, thus altering vascular tone and erectile function. Collectively, these studies unveil a possible mechanism that may be important for the protection of the tunica albuginea and corpus cavernosum from collagen changes, and also a possible mechanism by which there are alterations in the erectile physiology in Peyronie's disease patients.…”

Section: Nitric Oxide Synthase and Peyronie's Diseasementioning

confidence: 99%

Implications of nitric oxide synthase isoforms in the pathophysiology of Peyronie's disease

Bivalacqua

Champion

2002

Int J Impot Res

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“…Apoptosis is the most frequently reported mode of peroxynitrite-induced cell death (32)(33)(34)(35)(36)(37)(38)(39)(40); other studies, however, have shown that peroxynitrite leads to necrosis (41) or to both modes of cell death (42,43). These discrepancies are a possible consequence of differences in the peroxynitrite concentrations used and/or mode of peroxynitrite administration (e.g., as a precursor or as a bolus).…”

Section: Cell Death Induced By Peroxynitritementioning

confidence: 99%

Cell signaling and cytotoxicity by peroxynitrite.

Cantoni

Palomba

Guidarelli

et al. 2002

Environ Health Perspect

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Reactive nitrogen species are now considered to play an important role in various pathologies. Although the pathological significance of these molecules, peroxynitrite in particular, has long been attributed to their abilities to react with any component of the cells, including lipids, proteins, and DNA, a paradigm shift has recently been occurring whereby reactive nitrogen species are appreciated as signaling molecules. The question therefore arises as to whether nitrosative stress is indeed the result of a random (stochastic) process of cell damage, as it has traditionally been viewed, or rather is a consequence of the specific activation of a cascade of signaling events. The above considerations have provided the bases for the research work performed in our laboratory, and the results obtained are illustrated in the present article. In particular, our results indicate that some effects of peroxynitrite are not directly mediated by the oxidant; rather, it appears that peroxynitrite triggers a signaling pathway that finally leads to cytotoxicity.

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Reactive Oxygen Species Participate in Peroxynitrite-Induced Apoptosis in HL-60 Cells

Cited by 106 publications

References 24 publications

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Implications of nitric oxide synthase isoforms in the pathophysiology of Peyronie's disease

Cell signaling and cytotoxicity by peroxynitrite.

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