Reactive Oxygen Species Modulator 1 (ROMO1), a New Potential Target for Cancer Diagnosis and Treatment

Amini, Mohammad Amin; Talebi, Seyed Saman; Karimi, Jamshid

doi:10.4068/cmj.2019.55.3.136

Cited by 18 publications

(28 citation statements)

References 53 publications

(93 reference statements)

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“…In this experiment, CYP2E1 was upregulated in the H group. One of the most important proteins in the inner mitochondrial membrane is ROS modulator 1 ( ROMO1 ), which interferes with the production of ROS, and as the rate of this protein increases, oxidative stress increases, which ultimately leads to some diseases [ 56 ]. Studies have shown that the increase in Romo1 expression enhances cellular ROS levels and oxidative DNA damage [ 57 ], which consistent with our experimental results.…”

Section: Discussionmentioning

confidence: 99%

Differentially Expressed Hepatic Genes Revealed by Transcriptomics in Pigs with Different Liver Lipid Contents

Lyu

Xiang

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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The liver is the center for uptake, synthesis, packaging, and secretion of lipids and lipoproteins. The research on lipid metabolism in pigs is limited. The objective of the present study is to identify the genes related to lipid metabolism and oxidative stress in pigs by using transcriptomic analysis. Liver segments were collected from 60 Jinhua pigs for the determination of liver lipid content. The 7 pigs with the highest and lowest liver lipid content were set as group H and group L, respectively. Liver segments and serum samples were collected from each pig of the H and L groups for RNA sequencing and the determination of triglycerides (TG) content and high-density lipoprotein cholesterol (HDL) content, respectively. The HDL content in the serum of pigs in the H group was significantly higher than the L group ( P < 0.05 ). From transcriptomic sequencing, 6162 differentially expressed genes (DEGs) were identified, among which 2962 were upregulated and 3200 downregulated genes with the increase in the liver content of Jinhua pigs. After GO enrichment and KEGG analyses, lipid modification, cellular lipid metabolic process, cholesterol biosynthetic process, fatty acid metabolic process, oxidoreduction coenzyme metabolic process, oxidoreductase activity, acting on CH-OH group of donors, response to oxidative stress, nonalcoholic fatty liver disease (NAFLD), sphingolipid metabolism, and oxidative phosphorylation pathways were involved in lipid metabolism and oxidative stress in Jinhua pigs. For further validation, we selected 10 DEGs including 7 upregulated genes (APOE, APOA1, APOC3, LCAT, CYP2E1, GPX1, and ROMO1) and 4 downregulated genes (PPARA, PPARGC1A, and TXNIP) for RT-qPCR verification. To validate these results in other pig species, we analyzed these 10 DEGs in the liver of Duroc×Landrace×Yorkshire pigs. Similar expression patterns of these 10 DEGs were observed. These data would provide an insight to understand the gene functions regulating lipid metabolism and oxidative stress and would potentially provide theoretical basis for the development of strategies to modulate lipid metabolism and even control human diabetes and obesity by gene regulations.

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Section: Discussionmentioning

confidence: 99%

Differentially Expressed Hepatic Genes Revealed by Transcriptomics in Pigs with Different Liver Lipid Contents

Lyu

Xiang

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…In addition, it has been reported that high ROMO1 expression predicted unfavorable prognosis and lymphatic metastasis in NSCLC patients [ 20 ]. More importantly, ROMO1 has been reported to be a novel potential target for cancer diagnosis and treatment [ 21 ]. To investigate whether ROMO1 regulates the tumorigenesis of HB, both in vitro and in vivo experiments were performed.…”

Section: Discussionmentioning

confidence: 99%

High Expression of ROMO1 Aggravates the Malignancy of Hepatoblastoma

et al. 2021

Journal of Oncology

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Hepatoblastoma (HB) is a kind of tumor that occurs frequently in children and is highly malignant. Here, the function of ROS modulator 1 (ROMO1) was identified in the development of HB. In this study, the mRNA expression of ROMO1 was measured by RT-qPCR. Colony formation assay, MTT assay, and flow cytometric analysis were applied to detect cell viability. The cell migrative and invasive ability was measured by wound healing and transwell assays. Tumor xenografts were performed to examine tumor growth. The results showed that upregulation of ROMO1 was identified in liver hepatocellular carcinoma (LIHC) tissues and predicted poor prognosis in LIHC patients. And ROMO1 expression was also increased in HB tissues and cells. Functionally, ROMO1 knockdown restrained cell viability, migration, and invasion in HB. In addition, knockdown of ROMO1 was found to suppress tumor formation in vivo. In conclusion, upregulation of ROMO1 promotes tumor growth and cell aggressiveness in HB.

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“…One of the most important proteins that is involved in the production of Reactive Oxygen Species (ROS) is Reactive Oxygen Species modulator 1 (ROMO1). 6 7 Also known as Glyrichin, it has 79 amino acids and is located in chromosomal locus of 20q11.22. 6 ROMO1 is a mitochondrial protein that binds to some proteins of mitochondrial inner membrane and was first reported in various cancerous tissues which cause drug resistance.…”

Section: Introductionmentioning

confidence: 99%

The Association of COVID-19 and Reactive Oxygen Species Modulator 1 (ROMO1) with Oxidative Stress

et al. 2022

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There is no denying that the massive spread of COVID-19 around the world has worried everyone. The virus can cause mild to severe symptoms in various organs, especially the lungs. The virus affects oxidative stress in the cells. Reactive Oxygen Species modulator 1 (ROMO1) is one of the most important mitochondrial proteins that plays a critical regulatory role in the production of Reactive Oxygen Species (ROS). According to the studies, COVID-19 can promote oxidative stress through some important pathways, for instance, TNF-α and NF-κB routes. Furthermore, ROMO1 is closely related to these pathways and its dysfunction may affect these routes, then promote oxidative stress, and ultimately cause tissue damage, especially in the lungs. Another factor to consider is that the TNF-α and NF-κB pathways are associated with ROMO1, COVID-19, and oxidative stress. To summarize, it is hypothesized that COVID-19 may increase oxidative stress by affecting ROMO1. Understanding the exact molecular mechanisms of ROMO1 in the pathogenesis of COVID-19 can pave the way to find better therapeutic strategies.

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Reactive Oxygen Species Modulator 1 (ROMO1), a New Potential Target for Cancer Diagnosis and Treatment

Cited by 18 publications

References 53 publications

Differentially Expressed Hepatic Genes Revealed by Transcriptomics in Pigs with Different Liver Lipid Contents

Differentially Expressed Hepatic Genes Revealed by Transcriptomics in Pigs with Different Liver Lipid Contents

High Expression of ROMO1 Aggravates the Malignancy of Hepatoblastoma

The Association of COVID-19 and Reactive Oxygen Species Modulator 1 (ROMO1) with Oxidative Stress

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