Reactive oxygen species modulate Zn2+-induced apoptosis in cancer cells

Provinciali, Mauro; Donnini, Alessia; Argentati, Katy; Stasio, Grazia Di; Bartozzi, Beatrice; Bernardini, Giovanni

doi:10.1016/s0891-5849(01)00830-9

Cited by 54 publications

(38 citation statements)

References 33 publications

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“…One of the known mechanisms whereby zinc activates kinase signaling in exposed cells and exerts cytotoxicity is the generation of reactive oxygen species (ROS) (21). In line with this notion, we observed time-dependent elevation in oxidative stress in all colon cancer cell lines.…”

Section: Discussionsupporting

confidence: 83%

Diverse sensitivity of cells representing various stages of colon carcinogenesis to increased extracellular zinc: Implications for zinc chemoprevention

John

Briatka²,

Rudolf

2011

Oncol Rep

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Abstract.The relationship between zinc intake and risk of colon cancer is widely recognized. Despite reported mechanisms of zinc-mediated effects in colonic cells no information is available on whether zinc is capable of inducing cell death of malignant colonocytes. The present study shows that increased external zinc concentrations inhibit cell growth of three different colon cancer cell lines representing different stages of colon cancer: HCT-116, HT-29 and SW620 cells and induce their death. Of the tested cell lines, SW620 cells proved to be the most sensitive to externally added zinc and this sensitivity was at least partly due to increased levels of intracellular free zinc and the inability to overexpress metallothionein. Further studies into the mechanisms of zinc-induced cell injury and cell death revealed oxidative stress as the most important underlying mechanism activating stress kinase-dependent signaling, perturbation of mitochondria and plasma membrane damage. In addition, observed cell death in individual cell populations was cell line-dependent and variable including cells displaying features of apoptosis, necrosis, autophagy and other mixedtypes. In conclusion, presented results for the first time show variability of responses to zinc in colon cancer at different stages as modeled in vitro and suggest that zinc-induced cell death despite common underlying mechanism(s) might have a variable nature.

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Section: Discussionsupporting

confidence: 83%

Diverse sensitivity of cells representing various stages of colon carcinogenesis to increased extracellular zinc: Implications for zinc chemoprevention

John

Briatka²,

Rudolf

2011

Oncol Rep

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“…ROS include free radicals such as the superoxide anion, hydroxyl radicals, and the non-radical hydrogen peroxide (Adam-Vizi and Chinopoulos, 2006). In many experimental situations, induction of apoptosis is accompanied by an obvious rise in the intracellular ROS level (Kane et al, 1993;Morales et al, 1998;Park et al, 2007;Provinciali et al, 2002). Our study also demonstrated that intracellular peroxide levels rapidly increased after TTME treatment in a dose-dependent manner.…”

Section: Discussionsupporting

confidence: 62%

The Rhizome of Trillium tschonoskii Maxim. Extract Induces Apoptosis in Human Lung Cancer Cells

Huang

Zou

Xiong

2011

Zeitschrift Für Naturforschung C

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Trillium tschonoskii Maxim. has been used to treat several diseases including cancers in folk medicine. However, the mechanisms responsible for T. tschonoskii extract-induced apoptosis are not clear. This study was mainly undertaken to identify the major biochemical changes in a lung cancer cell line upon treatment with an T. tschonoskii extract (TTME), and to investigate the functional relationship between these changes. The n-butanol extract was used to evaluate the mechanism of induction of apoptosis in A549 human lung cancer cells and its effects on mitochondrial function and production of reactive oxygen species (ROS). The n-butanol extract of T. tschonoskii has cytotoxic, antiproliferative, and morphological effects on the lung cancer cell line. T. tschonoskii mainly leads to apoptosis of cancer cells with a concomitant increase in the release of cytochrome c and a loss of mitochondrial membrane potential in a dose-dependent manner. A rapid increase in the level of intracellular ROS and an accumulation of cells in the G2/M and S phase of the cell cycle were also observed in treated cells. These observations suggest that the n-butanol extract of T. tschonoskii has promising anticancer activities, which could be useful in cancer treatment.

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“…Nano-zinc oxide is a key element for maintenance of the structural and functional integrity of eukaryotic cells and tissues [19]. Many studies have addressed the importance of ZnO as an antioxidant and a therapeutic agent in several free radicals initiating systems [20][21][22][23][24] reported the antidiabetic effects of ZnONPs through induction of insulin, IR and glucose metabolizing enzymes gene expression. In the same line Umrani and Paknikar, [25] proved the ability of ZnONPs for controlling of blood glucose in diabetic rats, these are only two studies that monitored the effect of ZnONPs on diabetic rats, but there is not previous study carried out with F:ZnO and Cl:ZnO nanoparticles on diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Nano-doped Zinc Oxide and its Application as Protective Oxidative Changes in the Retina of Diabetic Rats

El-Rahman¹,

Sm²,

Al-Ghannam³

2016

J Diabetes Metab

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Oxidative stress is a basic mechanism behind the development of diabetic retinopathy (DR). Therefore, the present study conducted to evaluate the protective effect of zinc oxide nanoparticles (ZnONPs) doped with fluorine (F:ZnO) and chlorine (Cl:ZnO), on retinal oxidative stress in type II diabetic rats. TEM showed that the F:ZnO and Cl:ZnO have average particle size 17.7 and 59.3 nm, respectively. The results indicated that serum glucose was increased significantly (p ≤ 0.05) and serum insulin was decreased significantly 3 days after treatment with STZ compared to other groups. STZ treatment depleted both retinal and liver TBA and retinal GST contents. While, simultaneous treatment rats with low and high dose of STZ + F:ZnO and STZ + Cl:ZnO were decreased serum glucose induced by STZ and reversed the deplete influence on GSH level of retinal and reduced the highs in TBA levels of livers and retinas in STZ -administrated rats. The results investigate the useful effects of high dose of F:ZnO and Cl:ZnO in protection diabetic rats against hyperglycemia and retina against oxidative stress.

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Reactive oxygen species modulate Zn2+-induced apoptosis in cancer cells

Cited by 54 publications

References 33 publications

Diverse sensitivity of cells representing various stages of colon carcinogenesis to increased extracellular zinc: Implications for zinc chemoprevention

Diverse sensitivity of cells representing various stages of colon carcinogenesis to increased extracellular zinc: Implications for zinc chemoprevention

The Rhizome of Trillium tschonoskii Maxim. Extract Induces Apoptosis in Human Lung Cancer Cells

Synthesis and Characterization of Nano-doped Zinc Oxide and its Application as Protective Oxidative Changes in the Retina of Diabetic Rats

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