Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase

Bilodeau, Jean‐François; Blanchette, S.; Cormier, Nathaly; Sirard, Marc‐André

doi:10.1016/s0093-691x(01)00702-6

Cited by 108 publications

(86 citation statements)

References 39 publications

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“…Previous studies have reported that H 2 O 2 has a more adverse impact on frozen-thawed sperm than O -2 does. Bilodeau et al (2002) and Roca et al (2005) believe that TEY extender has a limited capacity to neutralize H 2 O 2 after thawing for bull semen. It appears that treatments III and V maintained intact membrane lipid composition by increasing GPX activity or decreasing the production of H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…The ejaculates were then pooled, equalizing the sperm contribution of each male to eliminate individual differences (Gil et al, 2003). The tris-egg-yolk (TEY) extender used in the study comprised 60 mM citric acid, 0.25 M tris-(hydroxymethyl)-aminomethane, 69 mM fructose, 20% egg yolk, 7% glycerol in 80 mL water or ROM, 250 mg L -1 gentamycin sulfate, 50 mg L -1 tylosin tartrate, 150 mg L -1 lincomycin hydrochloride, and 300 mg L -1 spectinomycin sulfate antibiotics (Bilodeau et al, 2002;Martínez et al, 2006).…”

Section: Experimental Animals Sampling Processing and Freeze-thaw Mmentioning

confidence: 99%

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Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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The present study determined the effects of the addition of rosemary extract (ROM), glutathione (GSH), and their combination (ROM + GSH) to freezing extender on the quality of bull semen after cryopreservation. Before cryoperservation, the samples were diluted in a tris-egg yolk (TEY) extender containing 5 mM GSH (treatment I), 5 or 10 g L–1 ROM (treatments II and III), and ROM with GSH (5 mM GSH with 5 or 10 g L–1 of ROM) (treatments IV and V). An extender containing no antioxidants (non-ROM/GSH-treated) served as control group. Kinematic parameters were evaluated by means of a computer-assisted semen analysis (CASA). The viability and membrane integrity of the sperm were assessed using eosin-nigrosin stain and the hypo-osmotic swelling test (HOST) at 0 and 2 h after freezethawing. Lipooxidative parameters, superoxide dismutase, and glutathione peroxidase (GPx) activity were assessed after thawing. Treatment III showed positive effects for total motility (TM) (p < 0.01), average path velocity (VAP) (p < 0.001), viability (p < 0.01) and HOST (p < 0.01); however, lipid peroxidation (LPO) decreased (p < 0.05) and GPx activity increased (p < 0.05) immediately after thawing compared to the control. The TM (p < 0.01), VAP (p < 0.01), viability (p < 0.01), HOST (p < 0.01) decreased in LPO (p < 0.01) and GPx activity (p < 0.05) for treatment V and the viability and GPx activity (p < 0.05) for treatment I were significantly higher than for the control group at 2 h after thawing. It was concluded that the inclusion of ROM and its combination with GSH improves the post-thaw quality of bull semen.

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Animals Sampling Processing and Freeze-thaw Mmentioning

confidence: 99%

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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“…It has been suggested that ROS induces membrane LPO in sperm and that the toxicity of the generated fatty acid peroxides are important causes of decreased sperm function, for example, motility [28,29]. Previous studies [8,28,30,31] had shown a correlation between high levels of ROS (superoxide, hydroxyl, hydrogen peroxide, nitric oxide and peroxynitrile) and decreased sperm motility. De Lamirande and Gagnon [32] also showed that ROS causes sperm immotility within 5-30 min, depending on the concentration.…”

Section: The Effects Of Superoxide On Sperm Motility Parametersmentioning

confidence: 99%

The in vitro effects of superoxide, some commercially available antioxidants and red palm oil on sperm motility

Aboua¹,

Plessis²,

Reichgelt³

et al. 2009

Asian J Androl

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“…These lipids make membranes susceptible to lipid peroxidation (LPO) and cause loss of semen function and death (Bilodeau et al, 2002). Reactive oxygen species (ROS) are formed during cryopreservation and the antioxidants can counteract the negative effects of ROS during this process (Thuwanut et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

et al. 2012

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This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50), 100 (VE100) and 250 (VE250) µM of vitamin E, and 0.5 (RG0.5), 1.0 (RG1.0) and 1.5 (RG1.5) mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4 (100 ìL semen:400 μL cryoprotectant solution). The toxicity of the solutions was evaluated by investigating semen motility after 10 min in the solution. The rest of the diluted semen was placed into 0.5 mL straws maintained in nitrogen vapour for 24 hours and packed into a nitrogen liquid cylinder for four days. The samples were thawed in a water bath at 60°C for 8 s and the rate (%) and duration (s) of semen activation with distilled water or sodium bicarbonate was evaluated. In the toxicity test, we found that vitamin E and reduced glutathione were not toxic to curimba semen at any of the tested concentrations (P>0.05). The duration of motility was longer (P<0.05) in semen activated with sodium bicarbonate 1% (163 ± 11 s), which was considered the best activator for semen under these conditions. No significant differences were found between the cryoprotectant solutions used after freezing (P>0.05). Thus, the antioxidants vitamin E and reduced glutathione did not improve the quality of cryopreserved curimba semen, but they did not cause toxic effects to the semen in natura and they did not decrease its quality during cryopreservation.Este estudo avaliou a adição de antioxidantes vitamina E e glutationa reduzida no sêmen criopreservado de curimba (Prochilodus lineatus) e comparou solução de bicarbonato de sódio e água destilada como ativadores. O experimento foi conduzido na estação ambiental da CEMIG, em Itutinga-MG, entre Dezembro/2009 e Janeiro/2010. Sêmen de sete animais, com motilidade espermática acima de 80%, foi diluído em soluções crioprotetoras compostas por metanol 10% e lactose 15% em diferentes concentrações de antioxidantes: 50 (VE50), 100 (VE100) e 250 (VE250) µM de vitamina E, 0,5 (RG5.5), 1,0 (RG1.0) e 1,5 (RG1.5) mM glutationa reduzida e uma solução controle sem antioxidante. O sêmen foi diluído na proporção de 1:4 (100 µL de sêmen: 400 µL de solução crioprotetora). A toxicidade das soluções foi avaliada pela motilidade espermática após de 10 minutos em solução. O restante do sêmen diluído foi armazenado em palhetas de 0,5 mL mantidos em vapor de nitrogênio por 24 horas e estocado em cilindro de nitrogênio líquido por quatro dias. As amostras foram descongeladas em banho-maria a 60°C por 8 segundos e avaliada a taxa (%) e duração (s) pela ativaçã...

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Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase

Cited by 108 publications

References 39 publications

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

The in vitro effects of superoxide, some commercially available antioxidants and red palm oil on sperm motility

Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

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