Reactive Oxygen Species-mediated Immunity against Leishmania mexicana and Serratia marcescens in the Phlebotomine Sand Fly Lutzomyia longipalpis

Diaz-Albiter, Hector; Sant’Anna, Maurício Roberto Viana; Genta, Fernando Ariel; Dillon, Rod J.

doi:10.1074/jbc.m112.376095

Cited by 91 publications

(75 citation statements)

References 45 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3E) have close relationship with ROS degradation (36,44). Transcription of these three genes in integument was higher toward the end of the larval-larval molting stage (Fig.…”

Section: Successful Ecdysis Is the Results Of Systematic Regulation Bymentioning

confidence: 99%

Functional Analysis of Insect Molting Fluid Proteins on the Protection and Regulation of Ecdysis

Zhang

Lü

Kong

et al. 2014

Journal of Biological Chemistry

114

View full text Add to dashboard Cite

Background: Periodical ecdysis occurs in insects with molting fluids accumulated among the old and new cuticles. Results: Molting fluid is a mixture containing many unknown proteins to inhibit microbian infection and regulate ecdysis. Conclusion: Insects produce molting fluids for protecting delicate insects and guaranteeing successful ecdysis. Significance: Molting proteins may be targets useful for pesticide development in the future.

show abstract

“…3E) have close relationship with ROS degradation (36,44). Transcription of these three genes in integument was higher toward the end of the larval-larval molting stage (Fig.…”

Section: Successful Ecdysis Is the Results Of Systematic Regulation Bymentioning

confidence: 99%

Functional Analysis of Insect Molting Fluid Proteins on the Protection and Regulation of Ecdysis

Zhang

Lü

Kong

et al. 2014

Journal of Biological Chemistry

114

View full text Add to dashboard Cite

show abstract

“…2A-C) indicating that promastigotes do not increase glucose uptake under stressful conditions. Analysis of metabolic flux revealed that H 2 O 2 , menadione, and diamide stimulated a metabolic reconfiguration from glycolysis toward PPP indicated by marked increase in 14 CO 2 evolution from [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]D-glucose whereas CO 2 evolution after utilization of [6][7][8][9][10][11][12][13][14] C]D-glucose did not significantly alter in Figure 6. Characterization of G6PDH and TAL overexpressing (OE) cell lines against wild-type (WT) cells transfected with empty vector.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, methylene blue, known to create intracellular oxidative stress by disrupting NADPH:NADP ratio, also had an similar effect on L. mexicana promastigotes (30). Cells cotreated with NAC in case of exposure to H 2 O 2 and menadione and GSH in case of diamide showed a decrease in 14 CO 2 evolution from [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]D-glucose indicative of ROS-mediated shifting of metabolic flux toward PPP from glycolysis. The glucose consumption assay also reinforced our observation by demonstrating that the glucose uptake pattern (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…This is particularly true for parasites, which can successfully withstand the oxidative burst that is part of the mammalian host innate immunity upon infection. Oxidizing conditions are also faced in the fly vector along the digenetic life cycle (1). Nonetheless, many parasitic organisms are highly sensitive to oxidative stress, as observed for the intracellular stages of Plasmodium (2), Toxoplasma (3), Trypanosoma cruzi (4), and for extracellular stages like the Trypanosoma brucei (5) bloodstream form, and Leishmania promastigotes (6).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Metabolic reconfiguration of the central glucose metabolism: a crucial strategy ofLeishmania donovanifor its survival during oxidative stress

et al. 2015

View full text Add to dashboard Cite

Understanding the mechanism that allows the intracellular protozoan parasite Leishmania donovani (Ld) to respond to reactive oxygen species (ROS) is of increasing therapeutic importance because of the continuing resistance toward antileishmanial drugs and for determining the illusive survival strategy of these parasites. A shift in primary carbon metabolism is the fastest response to oxidative stress. A 14 CO 2 evolution study, expression of glucose transporters together with consumption assays, indicated a shift in metabolic flux of the parasites from glycolysis toward pentose phosphate pathway (PPP) when exposed to different oxidants in vitro/ex vivo. Changes in gene expression, protein levels, and enzyme activities all pointed to a metabolic reconfiguration of the central glucose metabolism in response to oxidants. Generation of glucose-6-phosphate dehydrogenase (G6PDH) (∼5-fold) and transaldolase (TAL) (∼4.2-fold) overexpressing Ld cells reaffirmed that lethal doses of ROS were counterbalanced by effective manipulation of NADPH:NADP + ratio and stringent maintenance of reduced thiol content. The extent of protein carbonylation and accumulation of lipid peroxidized products were also found to be less in overexpressed cell lines. Interestingly, the LD 50 of sodium antimony gluconate (SAG), amphotericin-B (AmB), and miltefosine were significantly high toward overexpressing parasites. Consequently, this study illustrates that Ld strategizes a metabolic reconfiguration for replenishment of NADPH pool to encounter oxidative

show abstract

“…Leishmania can manipulate the sand fly potentially threatening digestive proteases activity (Borovsky and Schlein 1983, Schlein et al 1983, Dillon and Lane 1993, Schlein and Jacobson 1998, Telleria et al 2010 and also can cause damage to the stomodeal valve of the fly (Schlein et al 1992, Rogers et al 2008, potentiating transmission. On the other hand, sand flies can mount an immune response to Leishmania infection (Boulanger et al 2004, Ramalho-Ortigão et al 2007, Jochim et al 2008, Pitaluga et al 2009, Diaz-Albiter et al 2012, Telleria et al 2012.…”

mentioning

confidence: 99%

The Flagellar Protein FLAG1/SMP1 is a Candidate forLeishmania–Sand Fly Interaction

Di-Blasi

Lobo

Nascimento

et al. 2015

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Leishmaniasis is a serious problem that affects mostly poor countries. Various species of Leishmania are the agents of the disease, which take different clinical manifestations. The parasite is transmitted by sandflies, predominantly from the Phlebotomus genus in the Old World and Lutzomyia in the New World. During development in the gut, Leishmania must survive various challenges, which include avoiding being expelled with blood remnants after digestion. It is believed that attachment to the gut epithelium is a necessary step for vector infection, and molecules from parasites and sand flies have been implicated in this attachment. In previous work, monoclonal antibodies were produced against Leishmania. Among these an antibody was obtained against Leishmania braziliensis flagella, which blocked the attachment of Leishmania panamensis flagella to Phlebotomus papatasi guts. The protein recognized by this antibody was identified and named FLAG1, and the complete FLAG1 gene sequence was obtained. This protein was later independently identified as a small, myristoylated protein and called SMP1, so from now on it will be denominated FLAG1/SMP1. The FLAG1/SMP1 gene is expressed in all developmental stages of the parasite, but has higher expression in promastigotes. The anti-FLAG1/SMP1 antibody recognized the flagellum of all Leishmania species tested and generated the expected band by western blots. This antibody was used in attachment and infection blocking experiments. Using the New World vector Lutzomyia longipalpis and Leishmania infantum chagasi, no inhibition of attachment ex vivo or infection in vivo was seen. On the other hand, when the Old World vectors P. papatasi and Leishmania major were used, a significant decrease of both attachment and infection were seen in the presence of the antibody. We propose that FLAG1/SMP1 is involved in the attachment/infection of Leishmania in the strict vector P. papatasi and not the permissive vector L. longipalpis.

show abstract

Reactive Oxygen Species-mediated Immunity against Leishmania mexicana and Serratia marcescens in the Phlebotomine Sand Fly Lutzomyia longipalpis

Cited by 91 publications

References 45 publications

Functional Analysis of Insect Molting Fluid Proteins on the Protection and Regulation of Ecdysis

Functional Analysis of Insect Molting Fluid Proteins on the Protection and Regulation of Ecdysis

Metabolic reconfiguration of the central glucose metabolism: a crucial strategy ofLeishmania donovanifor its survival during oxidative stress

The Flagellar Protein FLAG1/SMP1 is a Candidate forLeishmania–Sand Fly Interaction

Contact Info

Product

Resources

About