Reactive oxygen species mediate TGF-β1-induced plasminogen activator inhibitor-1 upregulation in mesangial cells

Jiang, Zongpei; Seo, Ji Yeon; Ha, Hunjoo; Lee, Eun Ah; Kim, Yu Seun; Han, Dong Cheol; Uh, Soo Tack; Park, Choon-Sik; Lee, Hi Bahl

doi:10.1016/j.bbrc.2003.08.102

Cited by 100 publications

(94 citation statements)

References 24 publications

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“…Near confluent NRK-52E cells were incubated with serum-free media for 24 h to arrest and synchronize the cell growth. After this time period, the media were changed to fresh serum-free media that contained various concentrations of recombinant human (33,35) and MAPK inhibitors from our preliminary study. Cells were processed for measurement of cellular ROS and expression of E-cadherin, ␣-SMA, ERK, p38 MAPK, and Smad 2 at the end of incubation period as described below.…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

“…Dichlorofluorescein (DCF)-sensitive cellular ROS in NRK52E cells were measured by a FACS (Becton Dickinson, Mountain View, CA) as described previously (33). In brief, cells in various time points after stimulation with serum-free TGF-␤1 or H 2 O 2 were washed with serumfree and phenol red-free DMEM and loaded with 5 M 5-(an-6)-chloromethyl-2Ј,7Ј-dichlorodihydrofluorescein diacetate (Molecular Probes, Eugene, OR).…”

Section: Assay Of Cellular Reactive Oxygen Speciesmentioning

confidence: 99%

“…We (33) recently demonstrated that TGF-␤1 upregulates plasminogen activator inhibitor-1 expression in glomerular mesangial cells through ROS. ROS are known to activate MAPK (34).…”

mentioning

confidence: 99%

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Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Rhyu¹,

Yang

Ha³

et al. 2005

Journal of the American Society of Nephrology

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484

389

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Section: Cell Culture and Treatmentmentioning

confidence: 99%

Section: Assay Of Cellular Reactive Oxygen Speciesmentioning

confidence: 99%

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Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Rhyu¹,

Yang

Ha³

et al. 2005

Journal of the American Society of Nephrology

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484

389

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show abstract

“…It is well known that renal PAI-1 expression is overexpressed in pathologic conditions associated with fibrosis including diabetic nephropathy [11]. PAI-1 is also overexpressed in renal cells cultured under diabetic conditions such as HG [25] and TGF-β1 [4,24,26]. Data from genetically modified mice such as PAI-1 overexpressed transgenic or null mice suggest PAI-1 as a therapeutic target for renal fibrosis.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmin activity in the culture media was determined as previously described [24] using a synthetic fluorometric plasmin substrate methoxysuccinyl-L-Ala-L-Phe-L-Lys-7-amido-4-methyl-coumarin. 25μl of 5 fold-concentrated media was mixed with 31.2μl of dH2O and 112.5μl of 0.2 M Tris-HCl, pH 7.4, containing 0.2 M NaCl.…”

Section: Easurement Of Plasmin Activitymentioning

confidence: 99%

Plasminogen Activator Inhibitor-1 Antisense Oligodeoxynucleotides Abrogate Mesangial Fibronectin Accumulation

Park

Seo

2010

Korean J Physiol Pharmacol

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Downregulation of catalase by reactive oxygen species via PI 3 kinase/Akt signaling in mesangial cells

Venkatesan

Mahimainathan

Das

et al. 2006

Journal Cellular Physiology

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Reactive oxygen species (ROS) contribute to many glomerular diseases by targeting mesangial cells. ROS have been shown to regulate expression of many antioxidant enzymes including catalase. The mechanism by which the expression of catalase protein is regulated by ROS is not precisely known. Here we report that increased intracellular ROS level by hydrogen peroxide (H(2)O(2)) reduced the expression of catalase. H(2)O(2) increased phosphorylation of Akt kinase in a dose-dependent and sustained manner with a concomitant increase in the phosphorylation of FoxO1 transcription factor. Further analysis revealed that H(2)O(2) promoted rapid activation of phosphatidylinositol (PI) 3 kinase. The PI 3 kinase inhibitor Ly294002 and expression of tumor suppressor protein PTEN inhibited Akt kinase activity, resulting in the attenuation of FoxO1 phosphorylation and preventing the downregulating effect of H(2)O(2) on catalase protein level. Dominant negative Akt attenuated the inhibitory effect of H(2)O(2) on expression of catalase. Constitutively active FoxO1 increased the expression of catalase. However, dominant negative FoxO1 inhibited catalase protein level. Catalase transcription was reduced by H(2)O(2) treatment. Furthermore, expression of dominant negative Akt and constitutively active FoxO1 increased catalase transcription, respectively. These results demonstrate that ROS downregulate the expression of catalase in mesangial cells by PI 3 kinase/Akt signaling via FoxO1 as a target.

show abstract

Reactive oxygen species mediate TGF-β1-induced plasminogen activator inhibitor-1 upregulation in mesangial cells

Cited by 100 publications

References 24 publications

Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Plasminogen Activator Inhibitor-1 Antisense Oligodeoxynucleotides Abrogate Mesangial Fibronectin Accumulation

Downregulation of catalase by reactive oxygen species via PI 3 kinase/Akt signaling in mesangial cells

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