Reactive oxygen species, lipid peroxidation, protein oxidation and antioxidative enzymes in dehydrating Karanj (Pongamia pinnata) seeds during storage

Sahu, Balram; Sahu, Alok Kumar; Thomas, Virginia L.; Naithani, S. C.

doi:10.1016/j.sajb.2017.06.030

Cited by 31 publications

(45 citation statements)

References 33 publications

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“…Reductions in seedling growth a result of controlled deterioration of seeds were effectively alleviated by seed priming. It has been reported that seed priming enhances antioxidant activities, nutrient uptake, photosynthesis, rate of cell division and cell elongation [13,[48][49][50][51].…”

Section: Effects Of Priming On Emergence and Later Seedling Growthmentioning

confidence: 99%

“…The "oxidative stress" model for seed deterioration suggests that deterioration is a result of the continued production of reactive oxygen species (ROS) such as superoxide (O 2 − ), hydrogen peroxide (H 2 O 2 ) and the hydroxyl radical (OH) [13,14]. These ROS damage many biomolecules and, in particular.…”

Section: Introductionmentioning

confidence: 99%

“…These ROS damage many biomolecules and, in particular. cause cellular dysfunction through lipid peroxidation [13,14]. Furthermore, when the moisture content of seeds rises above approximately (c.) 14%, for example during early stages of imbibition, lipid peroxidation also occurs through the activities of the enzyme lipoxygenase [15].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, when the moisture content of seeds rises above approximately (c.) 14%, for example during early stages of imbibition, lipid peroxidation also occurs through the activities of the enzyme lipoxygenase [15]. This enzyme causes the release of cytotoxic lipid peroxidation products such as malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) [13]. Seeds possess housekeeping enzymes which ameliorate the cytotoxic effects of ROS and lipid peroxidation products on seeds, including superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Cathodic Water Invigoration on the Emergence and Subsequent Growth of Controlled Deteriorated Pea and Pumpkin Seeds

Fatokun

Beckett

Varghese

et al. 2020

Plants

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The quality of seeds in gene banks gradually deteriorates during long-term storage, which is probably, at least in part, a result of the progressive development of oxidative stress. Here, we report a greenhouse study that was carried out to test whether a novel approach of seed invigoration using priming with cathodic water (cathodic portion of an electrolysed calcium magnesium solution) could improve seedling emergence and growth in two deteriorated crop seeds. Fresh seeds of Pisum sativum and Cucurbita pepo were subjected to controlled deterioration to 50% viability at 14% seed moisture content (fresh weight basis), 40 °C and 100% relative humidity. The deteriorated seeds were thereafter primed with cathodic water, calcium magnesium solution and deionized water. In addition, to study the mechanism of the impacts of invigoration, the effects of such priming on the lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) and on the reactive oxygen species (ROS) scavenging enzymes superoxide dismutase and catalase were also determined in the fresh and deteriorated seeds. All priming treatments improved seed emergence parameters, subsequent seedling photosynthesis and growth relative to the unprimed seeds. In general, cathodic water was most effective at invigorating deteriorated seeds. Analysis of the lipid peroxidation products and antioxidant enzyme activities in invigorated seeds provided support for the hypothesis that the effectiveness of cathodic water in invigoration of debilitated orthodox seeds in general and of pea and pumpkin seeds in particular derive from its ability to act as an antioxidant.

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Section: Effects Of Priming On Emergence and Later Seedling Growthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of Cathodic Water Invigoration on the Emergence and Subsequent Growth of Controlled Deteriorated Pea and Pumpkin Seeds

Fatokun

Beckett

Varghese

et al. 2020

Plants

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show abstract

“…Overexpression of Cu-Zn-SOD genes in tobacco seeds improved seed longevity and germination by attenuating the effects of oxidative stress produced by elongated storage conditions and harsh environmental stresses (Lee et al 2010). It was suggested that loss of seed viability with ageing is associated with a decrease in SOD activity (Lehner et al 2008;Bao et al 2011;Xia et al 2015;Sahu et al 2017b). In the present study, Cu-Zn SOD level decreased at 3°C, which is in agreement with the above results; however, it increased only at − 3°C after 12 months of storage.…”

Section: Proteins Involved In Stress Responsesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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& Key message Adequate storability of black poplar (Populus nigra L.) seeds at temperatures below 0°C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining longterm stability of dehydrated tissue. & Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. & Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. & Methods Dried seeds (7.1% MC) were stored at 3°C, − 3°C, − 20°C, and − 196°C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. & Results A germination test showed that seeds remained viable at − 3, − 20, and − 196°C. Storage at 3°C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. & Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.

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Pongamia pinnata Seed Oil

Thirugnanasambandham¹

2019

Fruit Oils: Chemistry and Functionality

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Reactive oxygen species, lipid peroxidation, protein oxidation and antioxidative enzymes in dehydrating Karanj (Pongamia pinnata) seeds during storage

Cited by 31 publications

References 33 publications

Influence of Cathodic Water Invigoration on the Emergence and Subsequent Growth of Controlled Deteriorated Pea and Pumpkin Seeds

Influence of Cathodic Water Invigoration on the Emergence and Subsequent Growth of Controlled Deteriorated Pea and Pumpkin Seeds

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pongamia pinnata Seed Oil

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