Reactive oxygen species-induced protein carbonylation promotes deterioration of physiological activity of wheat seeds

Li, Bang-Bang; Zhang, Shuai-Bing; Lv, Yangyong; Shan, Wei; Hu, Yuansen

doi:10.1371/journal.pone.0263553

Cited by 12 publications

(5 citation statements)

References 54 publications

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“…Protein carbonylation is a fingerprint of oxidative stress of biological systems and is considered the most damaging type of modification of proteins. 35 The aging of apple embryos, 36 the senescence of root nodules in beans, 16 and the loss of seed vigor 37 are correlated with the buildup of protein carbonylation. The carbonylated proteins are finally degraded or accumulate in cells, which causes dysfunction of cells exposed to ROS.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species

Sultana

Yamamoto²,

Biswas

et al. 2022

J. Agric. Food Chem.

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Reactive oxygen species (ROS) are critical factors that cause damage in salt-stressed plants, but their mechanisms of action in living cells are largely unknown. We investigated the roles of reactive carbonyl species (RCS), i.e., the lipid peroxide-derived α,β-unsaturated aldehydes and ketones, in plant growth retardation under salt stress. When Arabidopsis thaliana Col-0 seeds were exposed to 100 mM NaCl, germination was delayed and the levels of ROS, RCS, and protein carbonylation in the seedlings were increased. Adding the histidine-containing dipeptides carnosine, N-acetylcarnosine, and anserine, which are reported RCS scavengers, restored the germination speed and suppressed the increases in RCS and protein carbonylation but did not affect the ROS level. Increases in the levels of the RCS acrolein, crotonaldehyde, (E)-2-pentenal, and 4-hydroxy-(E)-2-nonenal were positively correlated with the delay of germination and growth inhibition. These RCS, generated downstream of ROS, are thus primarily responsible for the salt-stress symptoms of plants.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species

Sultana

Yamamoto²,

Biswas

et al. 2022

J. Agric. Food Chem.

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“…Researchers concluded that increased As accumulation drastically enhanced lipid peroxidation and protein carbonylation, affecting rice plants’ yield. Likewise, ROS-mediated protein carbonylation of enzymatic and non-enzymatic antioxidants invariably decreases seed vigor and other physiological activity of the seed of wheat plants [ 117 ]. Similarly, [ 118 ] identified 180 carbonylated proteins in ABA-treated Arabidopsis thaliana leaves using affinity chromatography that differentially regulated stomatal closure upon ABA supplementation.…”

Section: Crosstalk Between Ros/rns/rss In Plant Defensementioning

confidence: 99%

Free Radicals Mediated Redox Signaling in Plant Stress Tolerance

Krishna

Kaushik

2023

Life

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Abiotic and biotic stresses negatively affect plant cellular and biological processes, limiting their growth and productivity. Plants respond to these environmental cues and biotrophic attackers by activating intricate metabolic-molecular signaling networks precisely and coordinately. One of the initial signaling networks activated is involved in the generation of reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sulfur species (RSS). Recent research has exemplified that ROS below the threshold level can stimulate plant survival by modulating redox homeostasis and regulating various genes of the stress defense pathway. In contrast, RNS regulates the stress tolerance potential of crop plants by modulating post-translation modification processes, such as S-nitrosation and tyrosine nitration, improving the stability of protein and DNA and activating the expression of downstream stress-responsive genes. RSS has recently emerged as a new warrior in combating plant stress-induced oxidative damage by modulating various physiological and stress-related processes. Several recent findings have corroborated the existence of intertwined signaling of ROS/RNS/RSS, playing a substantial role in crop stress management. However, the molecular mechanisms underlying their remarkable effect are still unknown. This review comprehensively describes recent ROS/RNS/RSS biology advancements and how they can modulate cell signaling and gene regulation for abiotic stress management in crop plants. Further, the review summarizes the latest information on how these ROS/RNS/RSS signaling interacts with other plant growth regulators and modulates essential plant functions, particularly photosynthesis, cell growth, and apoptosis.

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“…Take 200 g seeds and place them in an environment of 50°C and 55% relative humidity to artificially aging for 42 days, and the germination rate was 12%. At least three independent experiments were performed in the determination of germination rate (Li et al, 2022).…”

Section: Wheat Seed and Artificial Aging Treatmentmentioning

confidence: 99%

Comprehensive proteome and lysine acetylome analysis after artificial aging reveals the key acetylated proteins involved in wheat seed oxidative stress response and energy production

Zhang

Shan

et al. 2022

Journal of Food Biochemistry

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Lysine acetylation is a common post‐translational modification of proteins within all organisms. However, quantitative acetylome characterization in wheat seed during aging in storage has not been reported. This study reports the first large‐scale acetylome analysis of wheat seeds after artificial aging treatment, using the quantitative proteomic approach. In total, 11,002 acetylation sites, corresponding to 4262 acetylated proteins were identified, of which 1207 acetylated sites, representing 783 acetylated proteins, were significantly more or less acetylated after artificial aging. Functional analysis demonstrated that the majority of the acetylated proteins are closely involved with cellular and metabolic functions. In particular, key enzymes in the oxidative stress response and energy metabolism were significantly differentially acetylated and appear to be heavily involved in wheat seed aging. The acetylome analysis was verified by quantitative real‐time PCR and enzyme activity determination. Lysine‐acetylation results in a weaker oxidative stress response and lower energy production efficiency, resulting in the apoptosis of wheat seed cells, insufficient energy supply at the germination stage, and consequently, marked loss of seed vigor. Practical applications It is known that the loss of protein function is an important reason for the decrease of seed vigor. Therefore, the change of protein function in the process of wheat seed aging was studied by proteome and lysine acetylome analysis technology. The results showed that the oxidation–reduction imbalance and the decrease of energy production efficiency of seeds were the important reasons for the decrease of their vigor. This will provide a new idea for green and safe storage of grain.

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Reactive oxygen species-induced protein carbonylation promotes deterioration of physiological activity of wheat seeds

Cited by 12 publications

References 54 publications

Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species

Histidine-Containing Dipeptides Mitigate Salt Stress in Plants by Scavenging Reactive Carbonyl Species

Free Radicals Mediated Redox Signaling in Plant Stress Tolerance

Comprehensive proteome and lysine acetylome analysis after artificial aging reveals the key acetylated proteins involved in wheat seed oxidative stress response and energy production

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