Reactive oxygen species generation in fungal development and pathogenesis

Tudzynski, Paul; Heller, Jens; Siegmund, Ulrike

doi:10.1016/j.mib.2012.10.002

Cited by 113 publications

(86 citation statements)

References 46 publications

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“…Localization of Nox2 within membrane-bound vesicles is similar to localization patterns of NoxA and NoxB to the endoplasmic reticulum (ER) observed in B. cinerea (21). Furthermore, Yno1 in yeast (30) and mammalian Nox4 also localize to the ER, suggesting that this may be an important site to ROS generation (21). Although we have not yet verified whether Nox2 acts within the ER, its intracellular location and association with the Pls1 tetraspanin suggests that it does act in a very different manner from Nox1, generating ROS within the appressorium to regulate Factin organization.…”

Section: Discussionsupporting

confidence: 61%

“…Conversely, Nox2 is present intracellularly and is differentially expressed during appressorium maturation. Localization of Nox2 within membrane-bound vesicles is similar to localization patterns of NoxA and NoxB to the endoplasmic reticulum (ER) observed in B. cinerea (21). Furthermore, Yno1 in yeast (30) and mammalian Nox4 also localize to the ER, suggesting that this may be an important site to ROS generation (21).…”

Section: Discussionsupporting

confidence: 57%

“…Mutants lacking PLS1 in these fungi strongly resemble nox2 (noxB) mutants, and there is a very close phylogenetic association between fungi possessing both Nox2 and Pls1 homologs (20). It has been proposed that Pls1 might therefore be associated with Nox2 activity or specific recruitment to its site of action (21). To test this idea, we therefore expressed LifeAct-RFP, Sep5-GFP, and Chm1-GFP in a M. oryzae Δpls1 mutant (Fig.…”

Section: Oryzae Nadph Oxidase Mutants Are Impaired In F-actin Orgamentioning

confidence: 99%

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NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus

Ryder

Dagdas

Mentlak

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

172

259

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The rice blast fungus Magnaporthe oryzae infects plants with a specialized cell called an appressorium, which uses turgor to drive a rigid penetration peg through the rice leaf cuticle. Here, we show that NADPH oxidases (Nox) are necessary for septin-mediated reorientation of the F-actin cytoskeleton to facilitate cuticle rupture and plant cell invasion. We report that the Nox2-NoxR complex spatially organizes a heteroligomeric septin ring at the appressorium pore, required for assembly of a toroidal F-actin network at the point of penetration peg emergence. Maintenance of the cortical F-actin network during plant infection independently requires Nox1, a second NADPH oxidase, which is necessary for penetration hypha elongation. Organization of F-actin in appressoria is disrupted by application of antioxidants, whereas latrunculinmediated depolymerization of appressorial F-actin is competitively inhibited by reactive oxygen species, providing evidence that regulated synthesis of reactive oxygen species by fungal NADPH oxidases directly controls septin and F-actin dynamics.pathogenicity | plant pathogen | tetraspanin | gelsolin | GTPase

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Section: Discussionsupporting

confidence: 61%

Section: Discussionsupporting

confidence: 57%

Section: Oryzae Nadph Oxidase Mutants Are Impaired In F-actin Orgamentioning

confidence: 99%

See 1 more Smart Citation

NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus

Ryder

Dagdas

Mentlak

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

172

259

View full text Add to dashboard Cite

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“…This can be in the form of encapsulation for microbes (O'Meara and Alspaugh 2012; Lemire et al 2012) or the formation of propagules such as spores or seeds (McKenney et al 2013;Nonogaki 2014). Typically, while in the avoiding or replicating state, metabolism is greatly reduced but not curtailed completely (Finch-Savage and Leubner-Metzger 2006), so there is ongoing ROS production (Tudzynski et al 2012).…”

Section: Biological Insight Into Crosstalk Between Met Oxidation and mentioning

confidence: 99%

Convergent signaling pathways—interaction between methionine oxidation and serine/threonine/tyrosine O-phosphorylation

Rao

Thelen

Miernyk

2015

Cell Stress and Chaperones

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Oxidation of methionine (Met) to Met sulfoxide (MetSO) is a frequently found reversible posttranslational modification. It has been presumed that the major functional role for oxidation-labile Met residues is to protect proteins/ cells from oxidative stress. However, Met oxidation has been established as a key mechanism for direct regulation of a wide range of protein functions and cellular processes. Furthermore, recent reports suggest an interaction between Met oxidation and O-phosphorylation. Such interactions are a potentially direct interface between redox sensing and signaling, and cellular protein kinase/phosphatase-based signaling. Herein, we describe the current state of Met oxidation research, provide some mechanistic insight into crosstalk between these two major posttranslational modifications, and consider the evolutionary significance and regulatory potential of this crosstalk.

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“…4 ROS also play a direct role in sexual development, hyphal growth and cytoskeleton organization in fungi. [5][6][7] In plants, growing evidence supports a role for ROS in an extensive range of environmental responses and developmental processes such as response to biotic and abiotic stresses, hormonal signaling, division, cell elongation, root development, apical dominance, tracheary element maturation, trichome development, senescence and programmed cell death. [8][9][10][11][12][13][14][15][16][17][18] This apparent biological paradox is based on the fact that ROS could work either as toxic agents or as regulating biomolecules depending basically on concentration and pulse duration.…”

mentioning

confidence: 99%

New insights into the functional roles of reactive oxygen species during embryo sac development and fertilization inArabidopsis thaliana

Martín

Distefano

Zabaleta

et al. 2013

Plant Signaling & Behavior

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The Apparent Paradox of ROSAs aerobic organisms, plants live in an oxygen rich environment. Inevitable, reactive oxygen species (ROS) by-products and their chemical reactions are part of their basic metabolism. Initially documented as a toxic consequence of aerobic metabolism, ROS can also work as signaling molecules regulating crucial developmental and physiological events in many different organisms. Plants, animals, and even fungi have evolved mechanisms in which ROS are used as messengers to fulfill an extensive range of key biological processes. In Drosophila melanogaster, a signaling role for ROS was demonstrated in the differentiation of common myeloid progenitors in hematopoietic cells. Previously considered as toxic by-products of aerobic metabolism, reactive oxygen species (ROS) are emerging as essential signaling molecules in eukaryotes. Recent evidence showed that maintenance of ROS homeostasis during female gametophyte development is crucial for embryo sac patterning and fertilization. Although ROS are exclusively detected in the central cell of mature embryo sacs, the study of mutants deficient in ROS homeostasis suggests that controlled oxidative bursts might take place earlier during gametophyte development. Also, a ROS burst that depends on pollination takes place inside the embryo sac. This oxidative response might be required for pollen tube growth arrest and for sperm cell release. in this mini-review, we will focus on new insights into the role of ROS during female gametophyte development and fertilization. Special focus will be made on the mitochondrial Mn-Superoxide dismutase (MSD1), which has been recently reported to be essential for maintaining ROS homeostasis during embryo sac formation.

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Reactive oxygen species generation in fungal development and pathogenesis

Cited by 113 publications

References 46 publications

NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus

NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus

Convergent signaling pathways—interaction between methionine oxidation and serine/threonine/tyrosine O-phosphorylation

New insights into the functional roles of reactive oxygen species during embryo sac development and fertilization inArabidopsis thaliana

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