Reactive oxygen species generation by reverse electron transfer at mitochondrial complex I under simulated early reperfusion conditions

Tabata Fukushima, Caio; Dancil, Ian-Shika; Clary, Hannah; Shah, Nidhi; Nadtochiy, Sergiy M.; Brookes, Paul S.

doi:10.1016/j.redox.2024.103047

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2024

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Cited by 2 publications

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A model of mitochondrial superoxide production during ischaemia-reperfusion injury for therapeutic development and mechanistic understanding

Sorby-Adams,

Prime,

Miljkovic

et al. 2024

Redox Biology

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A model of mitochondrial superoxide production during ischaemia-reperfusion injury for therapeutic development and mechanistic understanding

Sorby-Adams,

Prime,

Miljkovic

et al. 2024

Redox Biology

View full text Add to dashboard Cite

AEOL-Induced NRF2 Activation and DWORF Overexpression Mitigate Myocardial I/R Injury

Lax,

Lopez,

Ballester

et al. 2024

Preprint

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The causal relationship between the activation of NRF2 and the preservation of SERCA2a function in mitigating myocardial ischemia-reperfusion (mI/R) injury, along with the associated regulatory mechanisms, remains incompletely understood. The aim of this study was to characterize this relationship by testing the pharmacological repositioning of AEOL-10150 (AEOL) as a novel NRF2 activator. C57BL6/J, Nrf2 knockout (Nrf2−/−), and wild-type (Nrf2+/+) mice, as well as human induced pluripotent stem cell-derived cardiomyocytes (hiPSCMs) were subjected to I/R injury. Gain/loss of function techniques, RT-qPCR, western blotting, LC/MS/MS, and fluorescence spectroscopy were utilized. Cardiac dimensions and function were assessed by echocardiography. In the early stages of mI/R injury, AEOL administration reduced mitochondrial ROS production, decreased myocardial infarct size, and improved cardiac function. These effects were due to NRF2 activation, leading to the overexpression of the micro-peptide DWORF, consequently enhancing SERCA2a activity. The cardioprotective effect induced by AEOL was diminished in Nrf2−/− mice and in Nrf2/Dworf knockdown models in hiPSCMs subjected to simulated I/R injury. Our data show that AEOL-induced NRF2-mediated upregulation of DWORF disrupts the phospholamban-SERCA2a interaction, leading to enhanced SERCA2a activation and improved cardiac function. Taken together, our study reveals that AEOL-induced NRF2-mediated overexpression of DWORF enhances myocardial function through the activation of the SERCA2a offering promising therapeutic avenues for mI/R injury.

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Reactive oxygen species generation by reverse electron transfer at mitochondrial complex I under simulated early reperfusion conditions

Cited by 2 publications

References 71 publications

A model of mitochondrial superoxide production during ischaemia-reperfusion injury for therapeutic development and mechanistic understanding

A model of mitochondrial superoxide production during ischaemia-reperfusion injury for therapeutic development and mechanistic understanding

AEOL-Induced NRF2 Activation and DWORF Overexpression Mitigate Myocardial I/R Injury

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