Reactive oxygen species-evoked endoplasmic reticulum stress mediates 1-nitropyrene-induced epithelial-mesenchymal transition and pulmonary fibrosis

Fu, Lin; Zhao, Hui; Xiang, Ying; Xiang, Hui-Xian; Hu, Biao; Tan, Zhu-Xia; Xue, Lingxiao; Gao, Lan; Wang, Bo; Wang, Hua; Zhang, Cheng

doi:10.1016/j.envpol.2021.117134

Cited by 51 publications

(26 citation statements)

References 42 publications

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“…All selected 239 CAP patients in this research must meet certain diagnostic standard: In accord with the clinical manifestations of pneumonia and consistent with chest CT scans, mainly including (1) chest radiograph suggesting either a new patchy infiltrate, leaf or segment consolidation, ground glass opacity or interstitial change; (2) at least one of the following signs: the presence of cough, sputum production and dyspnoea; core body temperature higher than 38.0 °C; auscultatory findings of abnormal breath sounds and rales; or the counts of white blood cell more than 10 × 10 9 L or less than 4 × 10 9 L; (3) occurred in the community, rather than in a hospital [ 22 ]. The inclusion criteria were as follows: pneumonia was occurred in the community or within a definite incubation period after hospitalization; these candidates were not admitted to the hospital for the last three months; not pregnant; no other pulmonary diseases, such as pulmonary malignant tumor, pulmonary tuberculosis or immunodeficiency [ 23 , 24 ]; no antibiotic treatment or intervention before hospitalization. Peripheral blood samples were collected and anticoagulated with EDTA within 24 h after hospitalization.…”

Section: Methodsmentioning

confidence: 99%

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Serum interleukin-17 predicts severity and prognosis in patients with community acquired pneumonia: a prospective cohort study

Chen

Wang

et al. 2021

BMC Pulm Med

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Background Some studies previously demonstrated that interleukin-17 (IL-17) involves in pulmonary diseases progression. Nevertheless, the role of IL-17 in community-acquired pneumonia (CAP) remains unknown. This study aims to examine the correlations between serum IL-17 with the severity and prognosis in CAP patients through a prospective cohort study. Methods All 239 CAP patients were recruited. Serum IL-17 was detected by enzyme-linked immunosorbent assay (ELISA). The CAP severity was evaluated through CAP severity scores, including CURB-65, CRB-65, PSI, SMART-COP, CURXO and APACHE II. Results Serum IL-17 was gradually increased consistent with the severity of CAP. Correlative analysis suggested that serum IL-17 was associated with clinical physiologic indicators among CAP patients. Logistic regression indicated that serum IL-17 was positively related to CAP severity scores. Additionally, the prognostic outcomes were tracked among CAP patients. The levels of IL-17 on admission were significantly increased in CAP patients with ICU admission, mechanical ventilation, vasoactive agent, death and longer hospitalization days. Logistic regression analyses revealed serum higher IL-17 on admission elevated the risks of vasoactive agent usage and longer hospital stays in CAP patients. The cut-off concentrations of serum IL-17 for death, ICU admission, mechanical ventilation and ≥ 14 hospital stays were 86.80 ng/mL, 84.92 ng/mL, 84.92 ng/mL and 60.29 ng/mL respectively. Conclusions Serum IL-17 on admission is positively associated with the severity and poor prognosis among CAP patients, revealing that IL-17 may implicate in the pathological process of CAP. Therefore, serum IL-17 may become an effective biomarker for diagnosis, prognosis and therapy for CAP patients.

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Section: Methodsmentioning

confidence: 99%

“…IL-6 ELISA kits (JYM1942Hu) were obtained from Wuhan Colorful Gene Biological Technology Co. The concentrations of serum inflammatory cytokines were detected following the instructions closely [ 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Serum interleukin-17 predicts severity and prognosis in patients with community acquired pneumonia: a prospective cohort study

Chen

Wang

et al. 2021

BMC Pulm Med

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“…RAW264.7 murine macrophage-like cells purchased from the Bioresource Collection and Research Centre (BCRC, Hsinchu, Taiwan) were cultured in DMEM supplemented with 50 IU/mL penicillin and 100 µg/mL streptomycin at 37 • C in an atmosphere of 5% CO 2 and 95% air. RAW264.7 cells were plated at a density of 5 × 10 5 cells/mL for 12 h, then the medium was replaced with a serum-free DMEM medium [11,12]. After cell incubation with 1-NP at the concentrations of 0, 3, 10, 30, and 50 µM for 30 min or 12 h, supernatants were collected for an enzyme-linked immunosorbent assay (ELISA) and Griess reaction assays; the cells were harvested for a protein expression assay.…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

“…Known as a nitro-polycyclic aromatic hydrocarbon and the most abundant nitroarene in diesel engine exhaust, 1-nitropyrene (1-NP) results from the incomplete combustion of carbonaceous organic substances [6,7]. Genotoxicity and apoptosis are induced by 1-NP in macrophages, liver and lung epithelial cells, and hepatoma cells [8][9][10][11][12]. Chronic inflammation in pulmonary fibrosis is induced by 1-NP through endoplasmic reticulum stress in mice [13].…”

Section: Introductionmentioning

confidence: 99%

Proinflammatory Responses of 1-Nitropyrene against RAW264.7 Macrophages through Akt Phosphorylation and NF-κB Pathways

Tsai

Chen

Liu

et al. 2021

Toxics

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Air pollution is a major environmental and public health problem worldwide. A nitro-polycyclic aromatic hydrocarbon and the most abundant air pollutant in diesel engine exhaust, 1-nitropyrene (1-NP), is caused by the incomplete combustion of carbonaceous organic substances. Macrophages are effector cells of the innate immune cells that provide resistance in the peripheral tissue. The overactivation of macrophages results in inflammation. The generation of proinflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumour necrosis factor alpha, is induced by 1-NP in a concentration-dependent manner in macrophages. In this study, the production of proinflammatory mediators, such as nitrogen oxide and prostaglandin E2, was induced by 1-NP in a concentration-dependent manner through the expression of iNOS and COX2. The generation of proinflammatory cytokines, iNOS, and COX2 was induced by 1-NP through nuclear factor (NF)-κB p65 phosphorylation and the degradation of its upstream factor, IκB. Finally, Akt phosphorylation was induced by 1-NP in a concentration-dependent manner. These findings suggest that 1-NP exhibits a proinflammatory response through the NF-κB pathway activation due to Akt phosphorylation.

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“…com/). Inflammatory cytokines were detected in the collected sera based on the manufacturer's instructions with minor adjustments (29,30).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Associations of Serum S100A12 With Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Prospective Cohort Study

et al. 2021

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BackgroundPrevious studies indicated the calcium-binding protein S100A12 to be involved in the pathophysiology of pulmonary inflammatory diseases. However, the role of S100A12 has remained elusive in patients with community-acquired pneumonia (CAP). Therefore, the purpose of this prospective cohort study was to evaluate the association between serum S100A12 with severity and prognosis in CAP patients.MethodsTwo groups with either 239 CAP patients or 239 healthy controls were enrolled in our study. Fasting blood and clinical characteristics were collected. On admission, serum S100A12 was measured using enzyme-linked immunosorbent assay (ELISA).ResultsSerum S100A12 was increased in CAP patients compared to control subjects. Furthermore, serum S100A12 was elevated according to the severity of CAP. Correlative analysis suggested that the level of serum S100A12 was associated with blood routine indices, renal function markers, inflammatory cytokines and other clinical parameters among CAP patients. Additionally, linear and logistical regression analyses indicated that serum S100A12 was positively associated with CAP severity scores in CAP patients. In addition, the association of high serum S100A12 and prognosis was accessed using a follow-up research. Elevated serum S100A12 on admission increased the risk of death and hospital stay in CAP patients during hospitalization.ConclusionsElevated serum S100A12 on admission is positively associated with the severity and adverse prognosis in CAP patients, suggesting that S100A12 may involve in the pathophysiological process of CAP. The titre of serum S100A12 may be used as a biomarker for diagnosis and prognosis among CAP patients.

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Reactive oxygen species-evoked endoplasmic reticulum stress mediates 1-nitropyrene-induced epithelial-mesenchymal transition and pulmonary fibrosis

Cited by 51 publications

References 42 publications

Serum interleukin-17 predicts severity and prognosis in patients with community acquired pneumonia: a prospective cohort study

Serum interleukin-17 predicts severity and prognosis in patients with community acquired pneumonia: a prospective cohort study

Proinflammatory Responses of 1-Nitropyrene against RAW264.7 Macrophages through Akt Phosphorylation and NF-κB Pathways

Associations of Serum S100A12 With Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Prospective Cohort Study

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