2005
DOI: 10.1124/mol.105.011544
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Abstract: Reactive oxygen species (ROS) are produced by all aerobic cells and have been implicated in the regulation of diverse cellular functions, including intracellular signaling, transcription activation, proliferation, and apoptosis. Salvicine, a novel diterpenoid quinone compound, demonstrates a broad spectrum of antitumor activities. Although salvicine is known to trap the DNA-topoisomerase II (Topo II) complex and induce DNA double-strand breaks (DSBs), its precise antitumor mechanisms remain to be clarified. In… Show more

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Cited by 71 publications
(47 citation statements)
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References 35 publications
(47 reference statements)
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“…Neutral Single-Cell Gel Electrophoresis Assay HL-60 cells (5 Â 10 5 /mL) were treated with either R16 or amonafide for 1 h. DNA double-strand breaks (DSB) were evaluated by neutral single-cell gel electrophoresis assay, which was done according to the method developed by Singh (21) with slight modifications (22). A fluorescence microscope (Olympus BX51) was used to capture the images.…”
Section: Terminal Deoxynucleotidyl Transferase^mediated Nick End Labementioning
confidence: 99%
See 2 more Smart Citations
“…Neutral Single-Cell Gel Electrophoresis Assay HL-60 cells (5 Â 10 5 /mL) were treated with either R16 or amonafide for 1 h. DNA double-strand breaks (DSB) were evaluated by neutral single-cell gel electrophoresis assay, which was done according to the method developed by Singh (21) with slight modifications (22). A fluorescence microscope (Olympus BX51) was used to capture the images.…”
Section: Terminal Deoxynucleotidyl Transferase^mediated Nick End Labementioning
confidence: 99%
“…Trapped in Agarose DNA Immunostaining Assay Trapped in agarose DNA immunostaining (TARDIS) assay was done as previously reported (22,24). Briefly, untreated or treated cells were harvested and mixed with low-melting gel spreading on slides, followed by placing the slides in lysis buffer containing protease inhibitors.…”
Section: Terminal Deoxynucleotidyl Transferase^mediated Nick End Labementioning
confidence: 99%
See 1 more Smart Citation
“…Similar attenuation of double-strand breaks upon pretreatment with NAC and exposure to other established topoisomerase-II targeting drugs, like doxorubicin and newer agents like salvacine have been reported previously, but the mechanism of ROS generation in this metabolic context is not fully understood. 17,18 Mitoxantrone is not unique in its capacity as a ROS-generation facilitator, and this quality is shared with other groups of drugs, for example farnesyl-transferase inhibitors. 19 A limiting factor in the use of topoisomerase-II targeting drugs is the significant toxicity that develops with the cumulative use of these drugs.…”
Section: Wwwlandesbiosciencecom Cancer Biology and Therapy E2mentioning
confidence: 99%
“…Thus, there is an urgent need for the development of future treatment regimens that may reduce the total dose of these drugs by combining them with inhibitors of DNA repair, currently under investigation. [18][19][20] The study by Huang and colleagues provides important new insight into the molecular mechanism of action of these drugs, thus facilitating the development of new therapy protocols.…”
Section: Wwwlandesbiosciencecom Cancer Biology and Therapy E2mentioning
confidence: 99%