Reactive Oxygen Species as Possible Mediator of Antibacterial Activity of Parkia Javanica, Against Bacterial Species Predominantly Found in Chronic Wound.

Abstract

The crude methanol extract of Parkia javanica was screened for antibacterial activity. against bacterial species predominantly found in chronic wound, by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC 100 5-40 mg/ml in case of standard bacterial strains. The lag phase of all extract treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of crude extract. This study suggests that the crude methanol extract of Parkia javanica possesses promising antimicrobial substances which are having activity against Standard ATCC bacterial species and ROS induced DNA damage could be the possible mediator of its antimicrobial activity.

Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study

“…[36,37] Several studies have reported the antibacterial, anti-inflammatory, and wound healing activities of P. javanica bark. [38][39][40][41] The methanolic extract of P. javanica bark (MEPJB) has also been reported to inhibit various blood cell malignancies. [42] However, there is no scientific study on the anti-colon cancer activity of P. javanica bark.…”

Chloroform Fraction of Parkia Javanica Bark Possesses Antibacterial Activity Against Multidrug Resistant Gram Negative Bacteria Predominantly Found in Skin Wound

“…This plant is widely used by tribal population of northeast region of India 11,12,13 . In our previous study, it was found that, crude methanol extract of Parkia javanica possess antibacterial activity against both standard ATCC strains and MDR strains and ROS induced DNA damage may be possible mediator of antibacterial activity of this plant 14,15,16 . Therefore, this work has been undertaken to explore the active fraction of this study plant having antibacterial properties against gram negative multi drug resistant bacterial strains with possible mode of action.…”