Reactive oxygen species and the strategy of antioxidant defense in fungi: A review

Белозерская, Т.А.; Гесслер, Н. Н.

doi:10.1134/s0003683807050031

Cited by 63 publications

(62 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SOD rapidly dismutates O 2˙Ϫ into H 2 O 2 , which is further reduced to H 2 O by both CAT and GPx, besides peroxiredoxins. Otherwise, in the presence of Fe 2ϩ , H 2 O 2 undergoes the Fenton reaction to generate the superoxidizing hydroxyl radical (OH˙) (41,42).…”

Section: Discussionmentioning

confidence: 99%

Meglumine Antimoniate (Glucantime) Causes Oxidative Stress-Derived DNA Damage in BALB/c Mice Infected by Leishmania (Leishmania) infantum

Moreira

Jesus

Soares

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Leishmaniasis is a neglected tropical disease caused by Ͼ20 species of the protozoan parasite Leishmania. Meglumine antimoniate (Glucantime) is the firstchoice drug recommended by the World Health Organization for the treatment of all types of leishmaniasis. However, the mechanisms of action and toxicity of pentavalent antimonials, including genotoxic effects, remain unclear. Therefore, the mechanism by which meglumine antimoniate causes DNA damage was investigated for BALB/c mice infected by Leishmania (Leishmania) infantum and treated with meglumine antimoniate (20 mg/kg for 20 days). DNA damage was analyzed by a comet assay using mouse leukocytes. Furthermore, comet assays were followed by treatment with formamidopyrimidine-DNA glycosylase and endonuclease III, which remove oxidized DNA bases. In addition, the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in the animals' sera were assessed. To investigate mutagenicity, we carried out a micronucleus test. Our data demonstrate that meglumine antimoniate, as well as L. infantum infection, induces DNA damage in mammalian cells by the oxidation of nitrogenous bases. Additionally, the antileishmanial increased the frequency of micronucleated cells, confirming its mutagenic potential. According to our data, both meglumine antimoniate treatment and L. infantum infection promote oxidative stress-derived DNA damage, which promotes overactivation of the SOD-CAT axis, whereas the SOD-GPx axis is inhibited as a probable consequence of glutathione (GSH) depletion. Finally, our data enable us to suggest that a meglumine antimoniate regimen, as recommended by the World Health Organization, would compromise GPx activity, leading to the saturation of antioxidant defense systems that use thiol groups, and might be harmful to patients under treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Meglumine Antimoniate (Glucantime) Causes Oxidative Stress-Derived DNA Damage in BALB/c Mice Infected by Leishmania (Leishmania) infantum

Moreira

Jesus

Soares

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The antioxidant system in fungi was recently reviewed in detail (14,53). In the following section, relevant data provided from Neurospora crassa are summarized (Fig.…”

Section: Antioxidant System In Neurospora Crassamentioning

confidence: 99%

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Gyöngyösi

Káldi

2014

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

Significance: Both circadian rhythm and the production of reactive oxygen species (ROS) are fundamental features of aerobic eukaryotic cells. The circadian clock enhances the fitness of organisms by enabling them to anticipate cycling changes in the surroundings. ROS generation in the cell is often altered in response to environmental changes, but oscillations in ROS levels may also reflect endogenous metabolic fluctuations governed by the circadian clock. On the other hand, an effective regulation and timing of antioxidant mechanisms may be crucial in the defense of cellular integrity. Thus, an interaction between the circadian timekeeping machinery and ROS homeostasis or signaling in both directions may be of advantage at all phylogenetic levels. Recent Advances: The Frequency-White Collar-1 and White Collar-2 oscillator (FWO) of the filamentous fungus Neurospora crassa is well characterized at the molecular level. Several members of the ROS homeostasis were found to be controlled by the circadian clock, and ROS levels display circadian rhythm in Neurospora. On the other hand, multiple data indicate that ROS affect the molecular oscillator. Critical Issues: Increasing evidence suggests the interplay between ROS homeostasis and oscillators that may be partially or fully independent of the FWO. In addition, ROS may be part of a complex cellular network synchronizing non-transcriptional oscillators with timekeeping machineries based on the classical transcription-translation feedback mechanism. Future Directions: Further investigations are needed to clarify how the different layers of the bidirectional interactions between ROS homeostasis and circadian regulation are interconnected. Antioxid. Redox Signal. 20,[3007][3008][3009][3010][3011][3012][3013][3014][3015][3016][3017][3018][3019][3020][3021][3022][3023]

show abstract

“…Two of the most abundant cDNAs in the library (contigs 246 and 225) encoded a putative glutathione S-transferase and S-formylglutathione hydrolase, both of which are involved in the metabolism of glutathione, which functions in detoxification and the regulation of intracellular redox status (Belozerskaya & Gessler, 2007). Other transcripts with potential roles in metabolism of reactive oxygen species or redox homeostasis included a putative Cu/Zn superoxide dismutase, a catalase (1-L10-SP6/T7) and a homologue of the cAMP response element (CREB-like) transcription factor CPTF1, which regulates catalase activity and contributes to virulence in the biotrophic plant pathogen Claviceps purpurea (Nathues et al, 2004).…”

Section: Putative Virulence and Pathogenicity Genesmentioning

confidence: 99%

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

et al. 2008

View full text Add to dashboard Cite

The hemibiotrophic ascomycete Colletotrichum higginsianum causes anthracnose disease on brassica crops and the model plant Arabidopsis. Melanized appressoria pierce the host cuticle and cell wall to form specialized biotrophic hyphae inside living epidermal cells. To identify proteins secreted by appressoria that may function as virulence effectors, a cDNA library was prepared from mature appressoria formed in vitro. Bidirectional sequencing of 980 clones generated 1442 high-quality expressed sequence tags (ESTs), comprising 518 unique sequences. BLASTX analysis showed that 353 (68 %) of these had significant similarity to entries in the NCBI non-redundant protein database, of which 49 were also homologous to experimentally verified fungal pathogenicity genes. ORFs were predicted ab initio from the unique sequences and screened for potential signal peptides using SignalP. Fifty-three unique sequences (10 %) were predicted to encode proteins entering the secretory pathway, of which 26 were likely to be soluble secreted proteins. For a selected subset of these, RT-PCR showed that seven genes that encode secreted proteins of unknown function, including two Colletotrichum-specific genes, are upregulated in appressoria and expressed early during plant infection, and therefore represent candidate effectors. INTRODUCTIONThe ascomycete genus Colletotrichum comprises one of the most economically damaging groups of plant-pathogenic fungi, causing anthracnose diseases and blights on an enormous range of dicot and monocot crop plants in temperate, tropical and subtropical regions (Bailey & Jeger, 1992). The crucifer anthracnose pathogen Colletotrichum higginsianum has a wide host range, attacking many cultivated forms of Brassica and Raphanus as well as wild crucifers, including the model plant Arabidopsis thaliana (Narusaka et al., 2004;O'Connell et al., 2004). C. higginsianum employs a two-stage, hemibiotrophic infection strategy: after melanized appressoria breach the host cuticle and cell wall, the fungus initially grows biotrophically inside living epidermal cells, before entering a destructive necrotrophic phase in which host tissues are killed and extensively macerated by cell-wall-degrading enzymes. During the biotrophic phase, C. higginsianum differentiates specialized intracellular primary hyphae similar to haustoria, which expand and locally modify the host plasma membrane (Shimada et al., 2006). However, in contrast to obligately biotrophic pathogens of Arabidopsis, Colletotrichum can be cultured axenically and stably transformed using a variety of methods; it is also a haploid organism with uninucleate conidia, which facilitates mutational analyses. This pathosystem therefore provides an attractive model for studying biotrophy, in which both partners in the interaction are genetically tractable.Whole-genome sequencing projects have revealed that fungal and oomycete plant pathogens possess large repertoires of secreted proteins, which can play diverse roles in pathogenicity and interactions with host cells (Dean...

show abstract

Reactive oxygen species and the strategy of antioxidant defense in fungi: A review

Cited by 63 publications

References 72 publications

Meglumine Antimoniate (Glucantime) Causes Oxidative Stress-Derived DNA Damage in BALB/c Mice Infected by Leishmania (Leishmania) infantum

Meglumine Antimoniate (Glucantime) Causes Oxidative Stress-Derived DNA Damage in BALB/c Mice Infected by Leishmania (Leishmania) infantum

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

Contact Info

Product

Resources

About