Reactive Oxygen Species and low-dose effects of tritium on bacterial cells

Rozhko, T.V.; Nogovitsyna, Evdokiya I.; Бадун, Г.А.; Lukyanchuk, Aleksandra N.; Kudryasheva, Nadezhda S.

doi:10.1016/j.jenvrad.2019.106035

Cited by 20 publications

(40 citation statements)

References 32 publications

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“…An increase in the bacterial luminescence intensity in the presence of tritiated water, HTO, was demonstrated in a series of experiments. The biphasic dependence (activation + inhibition) was found in [24,26], while the monophasic dependence (activation only) was shown in [30,32,33].…”

Section: ⎯⎯mentioning

confidence: 94%

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Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

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The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon.

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Section: ⎯⎯mentioning

confidence: 94%

“…Such a complex response was described in terms of the hormesis model. A signaling role of reactive oxygen species in the low-dose effects of tritium and americium-241 was considered in [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

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“…Original approach was applied in [64] [69] demonstrated that the exposure of marine bacteria to low-intensity irradiation of tritium increases ROS content in the bacterial environment considerably; and a rise of the ROS content correlates with intensification of bacterial bioluminescence intensity. These correlations were explained with "trigger" function of products of tritium decay, signaling role of ROS, and "bystander effect" in the bacterial suspension.…”

Section: Luminescence Bioassays As Tools For Study Low-dose Radiationmentioning

confidence: 99%

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

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Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

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“…Передавання ефекту свідка можливе як при безпосередньому контакті опромінених і неопромінених клітин (через щілинні міжклітинні контакти), так і через медіатори, що виділяються в культуральне середовище. При пророщуванні опроміненого і неопроміненого насіння в одному водному середовищі передавання радіаційного ефекту, очевидно, здійснюється деякими медіаторами, якими можуть бути, наприклад, активні форми кисню (Rozhko et al, 2019), гормони (цитокініни і ауксини). Концентрація і спектр речовин, що виділяються у водне середовище при проростанні опроміненого в дозі 40 Гр насіння, виявилися достатніми для формування ефекту свідка у неопроміненого насіння, але інтенсивність цього ефекту залежить від генотипу сорта і дози опромінення.…”

Section: таблиця 1 дисперсійний аналіз впливу сорту і сумісного прорunclassified

Induction of bystander effect in root meristem of soybean seedlings after γ-irradiation

Герман¹,

Легостаєва²,

Бабика³

2019

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

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The bystander effect refers to the non-target effects of ionizing radiation and it is the occurrence of radiation damages in the cells that have not been exposed to direct action of ionizing radiation. The aim of the study is to investigate the possibility of forming the “bystander effect” during the germination of irradiated and intact seeds of some soybean Glycine max (L.) Mer. varieties in the common aquatic environment. The soybean seeds of the Raiduga and Sprytna varieties (obtained by selection) and the genetically modified Apollo variety had been exposed to γ-radiation at a dose of 40 Gy. The mitotic activity of the seedlings root meristem cells of irradiated (IR) and intact (IN) seeds, as well as intact seeds, which were germinated in the same aqueous medium together with irradiated (INIR), was analyzed. The similar levels of mitotic activity were observed in seedlings of breeding varieties in “IN” variant, while cells in genetically modified variety divided more intensively. Radiation exposure in a dose of 40 Gy increased the level of mitotic activity in all varieties. The values of the mitotic indices increased 2 times in the varieties of Raiduga and Sprytna. The increase in Apollo variety was less significant, but this may be due to a high level of mitotic activity in the control variant. Irradiation also contributed to the appearance of chromosomal aberrations: fragments and bridges. The increase in mitotic activity in the meristem of the studied varieties to the mentioned levels may indicate the presence of a pool of meristem cells that can accelerate the passage of phases of the mitotic cycle under extreme conditions. Mitotic activity increased in the “INIR” variant in all investigated varieties. The largest excess over the “IN” was in Sprytna, a little less in the variety Raiduga. The proliferative activity in the Apollo variety meristem remained almost unchanged. Thus, the work shows the possibility of a “bystander effect” forming under the condition of joint germination of irradiated and intact seeds in the common aquatic environment. The intensity of “bystander effect” formation depends on the genotype and start mitotic potential.

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Reactive Oxygen Species and low-dose effects of tritium on bacterial cells

Cited by 20 publications

References 32 publications

Enzymatic Responses to Low-Intensity Radiation of Tritium

Enzymatic Responses to Low-Intensity Radiation of Tritium

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Induction of bystander effect in root meristem of soybean seedlings after γ-irradiation

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