Reactive oxygen species and colorectal cancer

Lin, Sisi; Li, Yongyu; Zamyatnin, Andrey A.; Werner, Jens; Bazhin, Alexandr V.

doi:10.1002/jcp.26356

Cited by 124 publications

(113 citation statements)

References 117 publications

(153 reference statements)

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“…Many investigations have found that the ROS levels in cancer cells are significantly associated with anticancer effect including proliferation inhibition and apoptosis induction. 30,[56][57][58][59] In order to explore the effect of Re-CDs on the inhibition of cancer cells, we measured the ROS level in A375 cells after they were treated with Re-CDs, by a flow cytometer. Results are displayed in Figure 6.…”

Section: Effect Of Re-cds On Ros Generation In Cancer Cellsmentioning

confidence: 99%

Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

Hua

Song

et al. 2018

IJN

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BackgroundFluorescent carbon-based nanomaterials have promising properties such as biosensing, cell imaging, tracing and drug delivery. However, carbon dots (CDs) with specific inherent biological functions have not been investigated. Ginsenosides are the components with multiple bioactivities found in plants of the genus Panax, which have attracted a lot of attention for their anticancer effect.Materials and methodsIn this study, we prepared a kind of novel photoluminescent CDs from ginsenoside Re by one-step hydrothermal synthesis method. The conventional methods including transmission electron microscopy, Fourier transform infrared spectroscopy, HPLC and fluorescence spectrum were used for characterization of CDs. In vitro anticancer effect was investigated by cytotoxicity assay, flow cytometry and Western blot analysis.ResultsThe as-prepared Re-CDs had an average diameter of 4.6±0.6 nm and excellent luminescent properties. Cellular uptake of Re-CDs was facilitated by their tiny nanosize, with evidence of their bright excitation-dependent fluorescent images. Compared with ginsenoside Re, the Re-CDs showed greater inhibition efficiency of cancer cell proliferation, with lower toxicity to the normal cells. The anticancer activity of Re-CDs was suggested to be associated with the generation of large amount of ROS and the caspase-3 related cell apoptosis.ConclusionHopefully, the dual functional Re-CDs, which could both exhibit bioimaging and anticancer effect, are expected to have great potential in future clinical applications.

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Section: Effect Of Re-cds On Ros Generation In Cancer Cellsmentioning

confidence: 99%

Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

Hua

Song

et al. 2018

IJN

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“…Higher than normal levels of ROS cause cell death; apoptosis, autophagy, and necrosis are the three main mechanisms that result in cell death. 66 Apoptosis can be triggered extrinsically by receptor-mediated pathways or intrinsically by mitochondria-mediated mechanisms. 67 Both intrinsic and extrinsic mechanisms are integrated to regulate cell apoptosis through mitochondria.…”

Section: Discussionmentioning

confidence: 99%

MRPL35 Is Up-Regulated in Colorectal Cancer and Regulates Colorectal Cancer Cell Growth and Apoptosis

Zhang

Yan

et al. 2019

The American Journal of Pathology

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Mitochondrial ribosome proteins (MRPs), which are encoded by the nuclear genomic DNA, are important for mitochondrial-encoded protein synthesis and mitochondrial function. Emerging evidence suggests that several MRPs also exhibit important extra-mitochondrial functions, such as involvement in apoptosis, protein biosynthesis, and signal transduction. In this study, we demonstrate a significant role of MRP L35 (MRPL35) in colorectal cancer (CRC). The expression of MRPL35 was higher in CRC tissues than in matched cancer-adjacent tissues and higher in CRC cells than in normal mucosal epithelial cells. Higher MRPL35 expression in CRC tissue correlated with shorter overall survival for CRC patients. In vitro, down-regulation of MRPL35 led to increased production of reactive oxygen species (ROS) together with DNA damage, loss of cell proliferation, G 2 /M arrest, a decrease in mitochondrial membrane potential, apoptosis, and autophagy induction. MRPL35 knockdown inhibited tumor proliferation in a CRC xenograft nude mouse model. Furthermore, overexpression of MRPL35 or treatment of cells with the ROS scavenger, N-acetyl cysteine, abrogated ROS production, cell cycle arrest, and apoptosis in vitro. These findings suggest that MRPL35 plays an essential role in the development of CRC and may be a potential therapeutic target for CRC. (Am J Pathol 2019, 189: 1105e1120; https:// Colorectal cancer (CRC), characterized by fast progression and poor prognosis, is one of the most common malignancies in the world. 1 The mechanisms of CRC development are still poorly understood. Although surgery is the most effective therapy for CRC, chemotherapy and radiotherapy are still indispensable. 2 Side effects and drug resistance are common issues with most of these treatments reducing their efficacy. As a consequence, CRC is in urgent need of markers for early diagnosis and of intervention targets for effective therapy.Mitochondria have a central role in cellular energy metabolism by oxidative phosphorylation, regulation of apoptosis, autophagy, and cell death. 3e5 Defective mitochondrial functions have been thought to be possible underlying mechanisms for cancer. 6,7 Mitochondria have their own DNA and ribosomes (mitoribosomes), which produce 13 proteins essential for oxidative phosphorylation. 8 Human mitoribosomes sediment as 55S particles, consisted of a 28S small subunit, formed by a 12S rRNA and 29 mitochondrial ribosome proteins (MRPs), and a 39S large subunit, formed by a 16S rRNA and 50 MRPs. 9,10 The approximately 70 MRPs are encoded by nuclear genomic DNA, synthesized in the cytosol,

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“…In TPs, the cases had a lower intake of tomato juice, apples, potatoes, bananas, strawberries, sweet potatoes, cabbages, radishes, catsup, watermelon, raisins, plums, peanuts, peaches, lemon juice, lettuce, red pepper, and soybeans than controls (p < 0.05) (Table S5). Although some epidemiological studies have reported conflicting data, there is strong mechanistic evidence for the effect of antioxidants on CRC induced by reactive oxygen species, demonstrating protective mechanisms that neutralize the production of free radicals [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Circulating Interleukin-6 Level, Dietary Antioxidant Capacity, and Risk of Colorectal Cancer

Kim

Lee

et al. 2019

Antioxidants

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Chronic inflammation is one of the causes of colorectal cancer (CRC), and circulating levels of inflammatory biomarkers have been linked to tumor promotion and progression. We aimed to evaluate the interleukin-6 (IL-6) level in CRC patients and determine whether a diet rich in antioxidants was associated with CRC. This study included 654 cases and 1312 controls matched for age and sex. We measured the plasma IL-6 concentration and estimated dietary antioxidant capacity based on oxygen radical absorbance capacity (ORAC) combined with a 106-item semiquantitative food frequency questionnaire. The IL-6 concentration was significantly increased in individuals with CRC (OR Q4 vs. Q1, 95% CI = 6.23, 4.10–9.45, p < 0.001). High dietary ORAC showed an inverse association with CRC (total ORAC OR Q4 vs. Q1, 95% CI = 0.26, 0.16–0.40, p < 0.001; total phenolics = 0.32, 0.21–0.50, p < 0.001). We found that low dietary ORAC was associated with a significant increase in CRC in the group with elevated IL-6 levels (total ORAC OR Q4 vs. Q1, 95% CI = 4.34, 3.12–6.02, p < 0.001; total phenolics = 4.61, 3.33–6.39, p < 0.001). This study suggested an inverse association between dietary antioxidant capacity and IL-6 level among patients with CRC.

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Reactive oxygen species and colorectal cancer

Cited by 124 publications

References 117 publications

Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

Synthesis of ginsenoside Re-based carbon dots applied for bioimaging and effective inhibition of cancer cells

MRPL35 Is Up-Regulated in Colorectal Cancer and Regulates Colorectal Cancer Cell Growth and Apoptosis

Circulating Interleukin-6 Level, Dietary Antioxidant Capacity, and Risk of Colorectal Cancer

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