Reactive oxygen species and cancer: A complex interaction

Saikolappan, Sankaralingam; Kumar, Binod; Shishodia, Gauri; Koul, Sweaty; Koul, Hari K.

doi:10.1016/j.canlet.2019.03.020

Cited by 186 publications

(151 citation statements)

References 134 publications

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“…Increasing evidence indicates a role for ROSassociated signalling in the proliferation, survival and other phenotypic behaviours of cancer cells. 32 In this study, we observed a marked decrease in ROS generation Figure 7 ROS markedly inhibits ROS generation and mediates the activation of pro-survival signalling pathways. Gastric cancer cells were treated with different doses of NAC for 12 h. (A) ROS production was determined using an ROS detection kit.…”

Section: Discussionsupporting

confidence: 50%

<p>Aloin Inhibits the Proliferation and Migration of Gastric Cancer Cells by Regulating NOX2–ROS-Mediated Pro-Survival Signal Pathways</p>

Wang¹,

Tang²,

Wang³

et al. 2020

DDDT

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Background: Aloin has been reported to have many pharmacological effects including antiinflammatory, anti-oxidant and anti-tumour activities. However, the precise molecular mechanisms underlying the anti-tumour properties of aloin are yet to be elucidated. Methods: HGC-27 and BGC-823 gastric cancer cells were treated with aloin. EdU and colony formation assays were used to detect the proliferation ability of cells. The migration of cells was detected using wound healing and transwell assays. Western blotting was used to detect the levels of cyclinD1, cyclin E1, MMPs, N-cadherin, E-cadherin and NOX2. The phosphorylation of Akt, mTOR, P70S6K, S6, Src, stat3 and IκBα were also detected by Western blotting. Flow cytometry was used to detect the cell cycle distribution.The location of p65 in cells was determined by using a confocal microscopy assay. The total amounts of ROS present in cells were measured using an ROS assay kit. Results: Here, we found that aloin inhibited the proliferation and migration of HGC-27 and BGC-823 gastric cancer cells using a combination of EdU, colony formation, wound healing and transwell assays. Further investigations revealed that aloin decreased the protein expression levels of cyclin D1, N-cadherin, and the matrix metalloproteinases (MMP)-2 and MMP-9; increased E-cadherin expression in a dose-dependent manner; inhibited reactive oxygen species (ROS) generation; and mediated the activation of Akt-mTOR, signal transducer and activator of transcription-3 (Stat3), and NF-κB signalling pathways. Our results also indicated that aloin is able to attenuate the expression levels of the two regulatory proteins of nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2), p47 phox and p22 phox , but had no effect on the level of gp91 phox. N-acetylcysteine treatment of gastric cancer cells inhibited ROS production and Akt-mTOR, Stat3, and IκBα phosphorylation. Taken together, our data suggest that aloin inhibits the proliferation and migration of gastric cancer cells by downregulating NOX2-ROS-mediated activation of the Akt-mTOR, Stat3, and NF-κB signalling pathways. Conclusion: Our findings suggest a potential role for aloin in the prevention of gastric cancer cell proliferation and migration and provide novel insights into the anti-cancer properties of aloin.

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Section: Discussionsupporting

confidence: 50%

<p>Aloin Inhibits the Proliferation and Migration of Gastric Cancer Cells by Regulating NOX2–ROS-Mediated Pro-Survival Signal Pathways</p>

Wang¹,

Tang²,

Wang³

et al. 2020

DDDT

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“…In addition, the development and metastasis of cancer are also closely related to the imbalance of the body’s redox balance [ 108 ]. This imbalance has been shown to be caused by an increase in free radicals (mainly ROS) [ 109 ].…”

Section: Effects Of Lipid Metabolism On Proliferation Migration mentioning

confidence: 99%

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

et al. 2020

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Lipids are essential components of cell structure and play important roles in signal transduction between cells and body metabolism. With the continuous development and innovation of lipidomics technology, many studies have shown that the relationship between lipids and cancer is steadily increasing, involving cancer occurrence, proliferation, migration, and apoptosis. Breast cancer has seriously affected the safety and quality of life of human beings worldwide and has become a significant public health problem in modern society, with an especially high incidence among women. Therefore, the issue has inspired scientific researchers to study the link between lipids and breast cancer. This article reviews the research progress of lipidomics, the biological characteristics of lipid molecules, and the relationship between some lipids and cancer drug resistance. Furthermore, this work summarizes the lipid molecules related to breast cancer diagnosis and prognosis, and then it clarifies their impact on the occurrence and development of breast cancer The discussion revolves around the current research hotspot long-chain non-coding RNAs (lncRNAs), summarizes and explains their impact on tumor lipid metabolism, and provides more scientific basis for future cancer research studies.

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“…In several animal experiments, an increase in antioxidant capacity has been suggested to lead to better cancer outcomes . ROS are produced in large amounts in proliferating cancer cells due to their exuberant metabolism, and cancer cells per se exhibit an enhanced antioxidant capacity to limit ROS generation because excessive ROS levels can induce cancer cell apoptosis . A targeted increase in ROS accumulation is a promising avenue for cancer treatment.…”

Section: The Dual Roles Of Reactive Species In Diseasementioning

confidence: 99%

Physiological regulation of reactive oxygen species in organisms based on their physicochemical properties

Huang

2019

Acta Physiologica

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Oxidative stress is recognized as free radical dyshomeostasis, which has damaging effects on proteins, lipids and DNA. However, during cell differentiation and proliferation and other normal physiological processes, free radicals play a pivotal role in message transmission and are considered important messengers. Organisms maintain free radical homeostasis through a sophisticated regulatory system in which these "2faced" molecules play appropriate roles under physiological and pathological conditions. Reactive oxygen species (ROS), including a large number of free radicals, act as redox signalling molecules in essential cellular signalling pathways, including cell differentiation and proliferation. However, excessive ROS levels can induce oxidative stress, which is an important risk factor for diabetes, cancer and cardiovascular disease. An overall comprehensive understanding of ROS is beneficial for understanding the pathogenesis of certain diseases and finding new therapeutic treatments.This review primarily focuses on ROS cellular localization, sources, chemistry and molecular targets to determine how to distinguish between the roles of ROS as messengers and in oxidative stress. K E Y W O R D Shomeostasis, oxidative stress, reactive oxygen, redox signalling, signalling pathways 2 of 16 | HUANG ANd LI for the development of new therapeutic strategies for cancer and cardiovascular disease. | SOURCES AND PHYSICOCHEMICAL PROPERTIES OF ROS | ROS sourcessecond messengers, what are the corresponding acceptors for these special ROS? To better recognize ROS, a new tool that can monitor changes in ROS levels and position in real time must be developed. Determining how to utilize the characteristics of ROS to treat diseases, such as cardiovascular disease, diabetes, autoimmune diseases, cancer and Alzheimer's diseases, is worth further exploration.

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Reactive oxygen species and cancer: A complex interaction

Cited by 186 publications

References 134 publications

<p>Aloin Inhibits the Proliferation and Migration of Gastric Cancer Cells by Regulating NOX2–ROS-Mediated Pro-Survival Signal Pathways</p>

<p>Aloin Inhibits the Proliferation and Migration of Gastric Cancer Cells by Regulating NOX2–ROS-Mediated Pro-Survival Signal Pathways</p>

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

Physiological regulation of reactive oxygen species in organisms based on their physicochemical properties

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