Reactive oxygen species acts as executor in radiation enhancement and autophagy inducing by AgNPs

Wu, Hao; Lin, Jun; Liu, Peidang; Huang, Zhihai; Zhao, Peng; Jin, Haizhen; Ma, Jun; Wen, Longping; Gu, Ning

doi:10.1016/j.biomaterials.2016.05.031

Cited by 106 publications

(85 citation statements)

References 44 publications

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“…The minimum dose of Ag NPs required to elicit significant TFEB translocation was 4 µg mL −1 (Figure d), similar to the dose required for autophagy induction as shown in Figure e, while 27 µg mL −1 PVP K30 failed to induce autophagy (Figure f). Previously, we have reported that reactive oxygen species act as executor in Ag NPs‐induced autophagy in U251 cells . 5 × 10 −3 m of N ‐acetyl cysteine (NAC), which effectively inhibited Ag NPs‐induced autophagy (Figure g), also blocked Ag NPs‐elicited TFEB nucleus translocation (Figure h and Figure S1, Supporting Information).…”

Section: Resultsmentioning

confidence: 90%

Key Role of TFEB Nucleus Translocation for Silver Nanoparticle‐Induced Cytoprotective Autophagy

Lin

Liu

et al. 2018

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Transcription factor EB (TFEB) is a master regulator of autophagy and lysosomal biogenesis. Here, silver nanoparticles (Ag NPs)-induced cytoprotective autophagy required TFEB is shown. Ag NPs-induced nucleus translocation of TFEB through a well-established mechanism involving dephosphorylation of TFEB at serine-142 and serine-211 but independent of both the mTORC1 and ERK1/2 pathways. TFEB nucleus translocation precedes autophagy induced by Ag NPs and leads to enhanced expression of autophagy-essential genes. Knocking down the expression of TFEB attenuates the autophagy induction is demonstrated, and in the meantime, enhanced cell killing in HeLa cells treats with Ag NPs, indicating that TFEB is the key mediator for Ag NPs-induced cytoprotective autophagy. The results pinpoint TFEB as a potential target for developing more effective Ag NPs-based cancer therapeutics.

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Section: Resultsmentioning

confidence: 90%

Key Role of TFEB Nucleus Translocation for Silver Nanoparticle‐Induced Cytoprotective Autophagy

Lin

Liu

et al. 2018

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“…45 For example, Ag nanoparticles can induce protective autophagy as a result of ROS upregulation when acting as a radiosensitizer. 46 In addition to the cytoprotective form of autophagy, the cytostatic form of autophagy can be induced to improve the radiation sensitivity of breast cancer cells. 47 In this study, we demonstrated that co-treatment with GONs enhanced autophagy in NH1299 cells; moreover, abrogation of autophagy by the 3-MA inhibitor substantially suppressed cell death.…”

Section: Discussionmentioning

confidence: 99%

<p>Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy</p>

Li¹,

Li²,

Jiang³

et al. 2019

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Background Gadolinium-based nanoparticles (GdNPs) have been used as theranostic sensitizers in clinical radiotherapy studies; however, the biomechanisms underlying the radio-sensitizing effects of GdNPs have yet to be determined. In this study, ultra-small gadolinium oxide nanocrystals (GONs) were employed to investigate their radiosensitizing effects and biological mechanisms in non-small-cell lung cancer (NSCLC) cells under X-ray irradiation. Method and materials GONs were synthesized using polyol method. Hydroxyl radical production, oxidative stress, and clonogenic survival after X-ray irradiation were used to evaluate the radiosensitizing effects of GONs. DNA double-strand breakage, cell cycle phase, and apoptosis and autophagy incidences were investigated in vitro to determine the radiosensitizing biomechanism of GONs under X-ray irradiation. Results GONs induced hydroxyl radical production and oxidative stress in a dose- and concentration-dependent manner in NSCLC cells after X-ray irradiation. The sensitizer enhancement ratios of GONs ranged between 19.3% and 26.3% for the NSCLC cells under investigation with a 10% survival rate compared with that of the cells treated with irradiation alone. Addition of 3-methyladenine to the cell medium decreased the incidence rate of autophagy and increased cell survival, supporting the idea that the GONs promoted cytostatic autophagy in NSCLC cells under X-ray irradiation. Conclusion This study examined the biological mechanisms underlying the radiosensitizing effects of GONs on NSCLC cells and presented the first evidence for the radiosensitizing effects of GONs via activation of cytostatic autophagy pathway following X-ray irradiation.

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“…Silver nanoparticles (Z = 47) have been investigated as [83] Copyright 2016, Elsevier. [87,117,118,121,122] For instance, it was reported that chitosan-coated Ag triangular nanoparticles exhibited better radio-sensitizing activity on human nonsmall lung cancer cells than that of PEG coated Au nanoparticles. [87,117,118,121,122] For instance, it was reported that chitosan-coated Ag triangular nanoparticles exhibited better radio-sensitizing activity on human nonsmall lung cancer cells than that of PEG coated Au nanoparticles.…”

Section: Nanoparticles For Radio-sensitization With Other Mechanismsmentioning

confidence: 99%

Emerging Nanotechnology and Advanced Materials for Cancer Radiation Therapy

et al. 2017

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Radiation therapy (RT) including external beam radiotherapy (EBRT) and internal radioisotope therapy (RIT) has been widely used for clinical cancer treatment. However, owing to the low radiation absorption of tumors, high doses of ionizing radiations are often needed during RT, leading to severe damages to normal tissues adjacent to tumors. Meanwhile, the RT efficacies are limited by different mechanisms, among which the tumor hypoxia-associated radiation resistance is a well-known one, as there exists hypoxia inside most solid tumors while oxygen is essential to enhance radiation-induced DNA damages. With the development in nanotechnology, there have been great interests in using nanomedicine strategies to enhance radiation responses of tumors. Nanomaterials containing high-Z elements to absorb radiation rays (e.g. X-ray) can act as radio-sensitizers to deposit radiation energy within tumors and promote treatment efficacy. Nanoscale carriers are able to deliver therapeutic radioisotopes into tumors for internal RIT, or chemotherapeutic drugs for synergistically combined chemo-radiotherapy. As uncovered in recent studies, the tumor microenvironment could be modulated by various nanomedicine approaches to overcome hypoxia-associated radiation resistance. Herein, the authors will summarize the applications of nanomedicine for RT cancer treatment, and pay particular attention to the latest development of 'advanced materials' for enhanced cancer RT.

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Reactive oxygen species acts as executor in radiation enhancement and autophagy inducing by AgNPs

Cited by 106 publications

References 44 publications

Key Role of TFEB Nucleus Translocation for Silver Nanoparticle‐Induced Cytoprotective Autophagy

Key Role of TFEB Nucleus Translocation for Silver Nanoparticle‐Induced Cytoprotective Autophagy

<p>Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy</p>

Emerging Nanotechnology and Advanced Materials for Cancer Radiation Therapy

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