Reactive oxygen species accumulation is synchronised with growth inhibition of temperature-sensitive recAts polA Escherichia coli

Kaidow, Akihiro; Ishii, Noriko; Suzuki, Shingo; Shiina, Takashi; Kasahara, Hirofumi

doi:10.1007/s00203-022-02957-z

Cited by 4 publications

(22 citation statements)

References 55 publications

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“…In a previous report, we demonstrated that recA polA cells were capable of colony formation in catalase-supplemented media either at a restrictive temperature (42 °C) [ 2 ] or in a rich medium [ 3 ]. Since catalase is a hydrogen peroxide-degrading enzyme, this suggested that the inability of recAts polA cells to grow at restrictive temperatures resulted from hydrogen peroxide accumulation.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study on recAts polA lethality reported that intracellular ROS accumulation was associated with lethality at the restrictive temperature [ 2 ]. However, why hslO was required to suppress recAts polA lethality was unclear.…”

Section: Discussionmentioning

confidence: 99%

“…We have reported in a previous paper that significant chromosome breakdown could not be observed [ 2 ]. However, we were not able to identify what causes growth failure of the recAts polA cells.…”

Section: Discussionmentioning

confidence: 99%

“…In combination with recombinase defects, chromosome breakage and double-strand break repair deficiencies render cells inviable. However, in Escherichia coli recAts polA cells, viability was retained when DNA damage response was evoked by constitutive expression of a lexA (Def) mutation [ 1 , 2 ]. We had previously shown that recA polA cell growth arrest was partially due to the accumulation of reactive oxygen species (ROS) and hydrogen peroxide under restrictive temperatures and rich medium conditions [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the complete absence of RecA function is tolerated in polA cells, indicating that recA polA cell mortality or growth arrest could be related to intracellular ROS levels rather than a deficiency in DSB repair. Furthermore, redox chaperone hslO is required for recA polA cells to survive [ 2 ]. However, the relationship between DNA damage, ROS production, and cell proliferation remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin C Maintenance against Cell Growth Arrest and Reactive Oxygen Species Accumulation in the Presence of Redox Molecular Chaperone hslO Gene

Kaidow

Ishii

Suzuki

et al. 2022

IJMS

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Chromosome damage combined with defective recombinase activity renders cells inviable, owing to deficient double-strand break repair. Despite this, recA polA cells grow well under either DNA damage response (SOS) conditions or catalase medium supplementation. Catalase treatments reduce intracellular reactive oxygen species (ROS) levels, suggesting that recA polA cells are susceptible to not only chronic chromosome damage but also ROS. In this study, we used a reducing agent, vitamin C, to confirm whether cell growth could be improved. Vitamin C reduced ROS levels and rescued colony formation in recAts polA cells under restrictive temperatures in the presence of hslO, the gene encoding a redox molecular chaperone. Subsequently, we investigated the role of hslO in the cell growth failure of recAts polA cells. The effects of vitamin C were observed in hslO+ cells; simultaneously, cells converged along several ploidies likely through a completion of replication, with the addition of vitamin C at restrictive temperatures. These results suggest that HslO could manage oxidative stress to an acceptable level, allowing for cell division as well as rescuing cell growth. Overall, ROS may regulate several processes, from damage response to cell division. Our results provide a basis for understanding the unsolved regulatory interplay of cellular processes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vitamin C Maintenance against Cell Growth Arrest and Reactive Oxygen Species Accumulation in the Presence of Redox Molecular Chaperone hslO Gene

Kaidow

Ishii

Suzuki

et al. 2022

IJMS

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HslO ameliorates arrested ΔrecA polA cell growth and reduces DNA damage and oxidative stress responses

Kaidow

Ishii

Suzuki

et al. 2022

Sci Rep

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Chromosome damage combined with defective recombinase activity has been widely considered to render cells inviable, owing to deficient double-strand break repair. However, temperature-sensitive recAts polA cells grow well upon induction of DNA damage and supplementation with catalase at restrictive temperatures. These treatments reduce intracellular reactive oxygen species (ROS) levels, which suggests that recAts polA cells are susceptible to ROS, but not chronic chromosome damage. Therefore, we investigated whether polA cells can tolerate a complete lack of recombinase function. We introduced a ΔrecA allele in polA cells in the presence or absence of the hslO-encoding redox molecular chaperon Hsp33 expression plasmid. Induction of the hslO gene with IPTG resulted in increased cell viability in ΔrecA polA cells with the hslO expression plasmid. ΔrecA polA cells in the absence of the hslO expression plasmid showed rich medium sensitivity with increasing ROS levels. Adding catalase to the culture medium considerably rescued growth arrest and decreased ROS. These results suggest that hslO expression manages oxidative stress to an acceptable level in cells with oxidative damage and rescues cell growth. Overall, ROS may regulate several processes, from damage response to cell division, via ROS-sensitive cell metabolism.

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Comparable antibacterial effects and action mechanisms of ethyl cyanoacrylate nanoparticles on Bacillus subtilis and Escherichia coli evaluated by transcriptome and morphological changes

Sarian

Ohama

2023

Environ. Sci.: Nano

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Reactive oxygen species accumulation is synchronised with growth inhibition of temperature-sensitive recAts polA Escherichia coli

Cited by 4 publications

References 55 publications

Vitamin C Maintenance against Cell Growth Arrest and Reactive Oxygen Species Accumulation in the Presence of Redox Molecular Chaperone hslO Gene

Vitamin C Maintenance against Cell Growth Arrest and Reactive Oxygen Species Accumulation in the Presence of Redox Molecular Chaperone hslO Gene

HslO ameliorates arrested ΔrecA polA cell growth and reduces DNA damage and oxidative stress responses

Comparable antibacterial effects and action mechanisms of ethyl cyanoacrylate nanoparticles on Bacillus subtilis and Escherichia coli evaluated by transcriptome and morphological changes

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