Reactive nitrogen species in cellular signaling

Adams, Levi; Franco, María Clara; Estévez, Álvaro G.

doi:10.1177/1535370215581314

Cited by 129 publications

(87 citation statements)

References 93 publications

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“…In our results, ONOO − aq is among the highest density of solvated RNS. (Peroxynitrite is a toxic RONS which can cause protein oxidation, lipid peroxidation, and DNA and mitochondrial damage, and is an antibacterial agent …”

Section: Plasma Jet Interaction With Water In a Well Plate—single‐dismentioning

confidence: 99%

“…[13,14] The interaction of plasma with cells may result in the oxidation of macromolecules and proteins, peroxidation of lipids, and damage to DNA which eventually interrupts cell signaling and metabolic processes. [15,16] The RONS produced by LTPs can increase the cells' oxidative stress leading to growth inhibition or cell death. [17][18][19] Many investigations have shown that there is a correlation between plasma produced RONS levels and cytotoxic effects.…”

Section: Introductionmentioning

confidence: 99%

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Helium plasma jet interactions with water in well plates

Mohades

Lietz

Kruszelnicki

et al. 2019

Plasma Processes & Polymers

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Plasma activation of liquids or cell cultures is a method for investigating the consequences of plasma produced reactive oxygen and nitrogen species (RONS) on living systems. The reproducible transfer of RONS, ions, electrons, and photons to the liquid is critical for determining reaction mechanisms and biological outcomes, and depends strongly on system parameters. A common in vitro method of plasma treatment of cells is a plasma jet directed into a well plate filled (or partially filled) with a liquid cell growth media. This method of treatment intrinsically has several environmental and geometrical factors that could lead to variability in the activation of the media. Uncontrolled or unreported geometrical and environmental factors that affect this transfer can, therefore, influence the reproducibility of measurements. In this paper, results from a numerical modeling investigation of a pulsed helium plasma jet interacting with water in a well plate are discussed while varying the height of the well‐plate rim. The height of the rim changes gas flow patterns, the ratio of He‐to‐air, and the water vapor content in the gas layer above the liquid. With a low rim, gas flowing from the jet stagnates on‐axis and flows radially outward with few vortices that recirculate reactants. With high rims, the gas flow is dominated by vortices and recirculation. With a low rim, the ionization wave (IW) from the jet strikes the liquid and proceeds as a surface IW across the water. With higher rims, the densities of helium and water vapor are higher above the liquid, which results in a volumetric propagation of the IW, initially producing higher densities of H2, HO2, OH, and H2O2. The end result is that for otherwise identical conditions, the densities of solvated H2O2aq and select reactive nitrogen species increase with rim height due to the vortices that recirculate reactants.

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Section: Plasma Jet Interaction With Water In a Well Plate—single‐dismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Helium plasma jet interactions with water in well plates

Mohades

Lietz

Kruszelnicki

et al. 2019

Plasma Processes & Polymers

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“…NO reacts with oxygen and reactive oxygen species to form reactive nitrogen species such as peroxynitrite and dinitrogen trioxide [4], which can affect protein function or expression. Reversible modification of proteins by NO via nitroslyation of cysteine residues has been likened to phosphorylation as a mode of cellular regulation.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates cellular degradation of human CYP51A1, the highly conserved lanosterol 14α-demethylase

Park

Byrd

Lee

et al. 2017

Biochemical Journal

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Nitric oxide (NO) is known to down-regulate drug metabolizing cytochrome P450 enzymes in an enzyme-selective manner. Ubiquitin-proteasome dependent and independent pathways have been reported. Here we studied the regulation of expression of human CYP51A1, the lanosterol 14α-demethylase required for synthesis of cholesterol and other sterols in mammals, which is found in every kingdom of life. In Huh7 human hepatoma cells, treatment with NO donors caused rapid post-translational down-regulation of CYP51A1 protein. Human nitric oxide synthase (NOS) – dependent down-regulation was also observed in cultured human hepatocytes treated with a cytokine mixture and in Huh7 cells expressing human NOS2 under control of a doxycycline-regulated promoter. This down-regulation was partially attenuated by proteasome inhibitors, but only trace levels of ubiquitination could be found. Further studies with inhibitors of other proteolytic pathways suggest a possible role for calpains, especially when the proteasome is inhibited. NO donors also down-regulated CYP51A1 mRNA in Huh7 cells, but to a lesser degree than the down-regulation of the protein.

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“…Oxidative stress is initiated by ROS such as perhydroxy radical (HOO À ), HO , and O 2 À , and by reactive nitrogen species (RNS) including nitric oxide (NO) and peroxynitrite (ONOO À ) (Adams et al, 2015;Mishra et al, 2014). Mitochondria were revealed to be the major cellular source for the generation of ROS and, at the same time, the main targets of ROS-induced oxidative damage (Tabassum et al, 2010;Zorov et al, 2014).…”

Section: Generation Of Ros and Oxidative Stressmentioning

confidence: 99%

The critical role of oxidative stress in the toxicity and metabolism of quinoxaline 1,4-di-N-oxides in vitro and in vivo

Wang

Martínez

Cheng

et al. 2016

Drug Metabolism Reviews

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Quinoxaline 1,4-dioxide derivatives (QdNOs) have been widely used as growth promoters and antibacterial agents. Carbadox (CBX), olaquindox (OLA), quinocetone (QCT), cyadox (CYA) and mequindox (MEQ) are the classical members of QdNOs. Some members of QdNOs are known to cause a variety of toxic effects. To date, however, almost no review has addressed the toxicity and metabolism of QdNOs in relation to oxidative stress. This review focused on the research progress associated with oxidative stress as a plausible mechanism for QdNO-induced toxicity and metabolism. The present review documented that the studies were performed over the past 10 years to interpret the generation of reactive oxygen species (ROS) and oxidative stress as the results of QdNO treatment and have correlated them with various types of QdNO toxicity, suggesting that oxidative stress plays critical roles in their toxicities. The major metabolic pathways of QdNOs are N→O group reduction and hydroxylation. Xanthine oxidoreductase (XOR), aldehyde oxidase (SsAOX1), carbonyl reductase (CBR1) and cytochrome P450 (CYP) enzymes were involved in the QdNOs metabolism. Further understanding the role of oxidative stress in QdNOs-induced toxicity will throw new light onto the use of antioxidants and scavengers of ROS as well as onto the blind spots of metabolism and the metabolizing enzymes of QdNOs. The present review might contribute to revealing the QdNOs toxicity, protecting against oxidative damage and helping to improve the rational use of concurrent drugs, while developing novel QdNO compounds with more efficient potentials and less toxic effects.

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Reactive nitrogen species in cellular signaling

Cited by 129 publications

References 93 publications

Helium plasma jet interactions with water in well plates

Helium plasma jet interactions with water in well plates

Nitric oxide stimulates cellular degradation of human CYP51A1, the highly conserved lanosterol 14α-demethylase

The critical role of oxidative stress in the toxicity and metabolism of quinoxaline 1,4-di-N-oxides in vitro and in vivo

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