Reactive extraction of fructose for efficient separation of sucrose-derived glucosides produced by enzymatic glycosylation

Abstract

<p>Reactive extraction enables efficient and selective separation of fructose from glucosides (here: α-glucosyl glycerol) produced from sucrose by enzymatic transglycosylation.</p>

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

“…Considering 2-GG synthesis integrated with product purification, the task reflects a real process need. Reported procedures of downstream processing (Kruschitz and Nidetzky 2020a ; Kruschitz and Nidetzky 2020b ) are incapable of separating sucrose and 2-GG. The trends of the data that conversion decreased with increasing space velocity and that the effect was stronger with bigger-sized particles were consistent with expectations from chemical engineering theory.…”

“…2-GG and fructose are the products (Goedl et al 2008 ; Luley-Goedl et al 2010 ). Of note, the product downstream processing can involve nanofiltration (Kruschitz and Nidetzky 2020a ), optionally combined with reactive extraction (Kruschitz and Nidetzky 2020b ). The biotransformation is performed with glycerol in excess (≥1.5-fold) over sucrose, to minimize donor hydrolysis resulting in glucose release (Scheme S1 ) (Goedl et al 2008 ).…”

Three‐level hybrid modeling for systematic optimization of biocatalytic synthesis: α‐glucosyl glycerol production by enzymatic trans‐glycosylation from sucrose

“…The conversion targets were defined as [GG] ≥ 983 mM (250 g/L), conversion of Suc () ≥ 0.98, and GG yield () ≥ 0.90. Requirements of the GG downstream processing, in particular the need to have Suc largely removed for its difficult separation from the GG, were taken into account (Kruschitz & Nidetzky, 2020a, 2020b). Results are shown in a two‐dimensional map (Figure 7), showing regions according to degree of fulfillment of the processing targets.…”

Biocatalytic Production of 2-α-d-Glucosyl-glycerol for Functional Ingredient Use: Integrated Process Design and Techno-Economic Assessment

“…The previously reported three-step extraction, based on a mixer-settler setup, was applied. 18 The whole process was split into six batches. For each batch, 0.42 L of the reaction mixture was diluted with 0.73 L 0.3 M Na 2 CO 3 /NaHCO 3 buffer (pH ∼ 10.6) and mixed with 1.15 L organic phase, made of octanol/heptane (4/1, v/v) containing ∼17 g L –1 naphthalene-2-boronic acid and ∼75 g L –1 Aliquat 336.…”

“… 17 Fructose is separated through a three-step reactive extraction-acidic stripping process. 18 From these earlier studies, a promising structure of the integrated 2-GG process is shown in Figure 1 . However, the different process steps have not been shown at a coordinated scale and interlinked into a complete production process.…”