Reactive carriers for the immobilization of copper ions

Walenius, Maria; Flodin, Per; Porath, Jerker

doi:10.1016/0021-9673(92)85522-u

Cited by 7 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metal chelate affinity chromatography introduces a new possibility for selectively interacting materials on the basis of their affinities for chelated transition metal ions. The separation is based on differential binding abilities of the proteins or enzymes to interact with chelated metal ions to a solid carrier [1,2]. Metal chelate affinity chromatography of proteins, with metal chelate linked to Sepharose, was first introduced by Porath et al [3].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels

Pekel¹,

Salih²,

Güven³

2005

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

Poly(N-vinylimidazole), PVIm, gels were prepared by gamma-irradiation polymerization of N-vinylimidazole in aqueous solutions as an affinity gel for glucose oxidase (GOx). These affinity gels with a water swelling ratio of 1800% for plain polymeric gel and between 30-80% for Cu(II) and Co(II)-chelated gels at pH 6.0 in phosphate buffer were used in the GOx adsorption studies. Maximum metal ion adsorption capacity of these hydrogels was found to be 3.64 mmol/g dry gel for Cu(II) and 1.72 mmol/g dry gel for Co(II) leading to GOx adsorption capacities of 343 and 528 mg enzyme/g dry gel, respectively, as compared to 228 mg for the plain dry PVIm gel. Activity studies were carried out using plain and the metal ion-chelated form of this hydrogel to investigate the stability and retained activity of the GOx in different buffer solutions and at different temperatures. Activity of the enzyme, either in free or immobilized form on the gel, decreased dramatically in acetate buffer solutions. In phosphate buffer solution, however, stability of enzyme has been found to be significantly high reaching 90% retained activity at the end of a 40-day period at 4 degrees C for Co(II) chelated systems. After immobilization of the enzyme onto metal-chelated hydrogel, the thermal stability of enzyme was enhanced significantly showing 23% activity, even at 75 degrees C.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels

Pekel¹,

Salih²,

Güven³

2005

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

show abstract

Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Cu(II): Effects of pH Value and Salt Concentration

Chen

Lee

et al. 1997

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

In this investigation, employing a highly sensitive microcalorimeter, we measure the influence of pH value and salt concentration on the heat of interaction between lysozyme and CS-IDA-Cu(II) gel. The direct enthalpy measurement of the interaction provides thermodynamic information regarding the binding behavior of lysozyme toward the immobilized metal ion. The binding enthalpy altered by adsorbed lysozyme at various pH values and salt concentrations are measured. The findings, along with the reported binding isotherm, are discussed herein.

show abstract

Selective and rapid uptake of Cu(II) by bis(benzimidazole) modified oxirane and thiirane resins

Berkel

Dijkstra

Driessen

et al. 1995

Reactive and Functional Polymers

View full text Add to dashboard Cite

Reactive carriers for the immobilization of copper ions

Cited by 7 publications

References 19 publications

Enhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels

Enhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels

Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Cu(II): Effects of pH Value and Salt Concentration

Selective and rapid uptake of Cu(II) by bis(benzimidazole) modified oxirane and thiirane resins

Contact Info

Product

Resources

About