Reactive astrogliosis induces astrocytic differentiation of adult neural stem/progenitor cells in vitro

Faijerson, Jonas; Tinsley, Rogan B.; Apricó, Karina; Thorsell, Annika; Nodin, Christina; Nilsson, Michael; Blomstrand, Fredrik; Eriksson, Peter S.

doi:10.1002/jnr.21044

Cited by 38 publications

(21 citation statements)

References 46 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cells lay extensive extracellular matrix to the wound site, which forms the gliotic scar that is impermeable to axon regeneration and neuronal cell infiltration (Bovolenta et al, 1992;Fawcett and Asher, 1999;Silver and Miller, 2004;Rolls et al, 2009). It is remarkable that the astrocytes, which form the scar tissue are very similar to the ones that constitute the neural stem cells or are found in the brain parenchyma (Liberto et al, 2004;Faijerson et al, 2006;Buffo et al, 2008;Robel et al, 2011). Indeed, reactive astrocytes can also generate neurons under certain conditions (Wilhelmsson et al, 2004;Mori et al, 2005;Berninger et al, 2007;Buffo et al, 2008;Robel et al, 2009;L'Episcopo et al, 2011;Heinrich et al, 2010;Blum et al, 2010).…”

Section: Stem/progenitor Cells In the Adult Vertebrate Brain Adult Nementioning

confidence: 96%

Adult neurogenesis and brain regeneration in zebrafish

Kızıl

Kaslin

Kroehne

et al. 2012

Developmental Neurobiology

300

307

View full text Add to dashboard Cite

Adult neurogenesis is a widespread trait of vertebrates; however, the degree of this ability and the underlying activity of the adult neural stem cells differ vastly among species. In contrast to mammals that have limited neurogenesis in their adult brains,zebrafish can constitutively produce new neurons along the whole rostrocaudal brain axis throughout its life.This feature of adult zebrafish brain relies on the presence of stem/progenitor cells that continuously proliferate,and the permissive environment of zebrafish brain for neurogenesis. Zebrafish has also an extensive regenerative capacity, which manifests itself in responding to central nervous system injuries by producing new neurons to replenish the lost ones. This ability makes zebrafish a useful model organism for understanding the stem cell activity in the brain, and the molecular programs required for central nervous system regeneration.In this review, we will discuss the current knowledge on the stem cell niches, the characteristics of the stem/progenitor cells, how they are regulated and their involvement in the regeneration response of the adult zebrafish brain. We will also emphasize the open questions that may help guide the future research.

show abstract

Section: Stem/progenitor Cells In the Adult Vertebrate Brain Adult Nementioning

confidence: 96%

Adult neurogenesis and brain regeneration in zebrafish

Kızıl

Kaslin

Kroehne

et al. 2012

Developmental Neurobiology

300

307

View full text Add to dashboard Cite

show abstract

“…The functions of these incompletely differentiated cells are not known. It is possible that this population of immature neurons underlies the ability of the frog's brain to undergo regeneration after injury, as it has been noted that injury can induce differentiation into both astroglia [Faijerson et al, 2006] and new neurons [Tseng et al, 2006] in vitro.…”

Section: Discussionmentioning

confidence: 99%

Cell Proliferation in the Forebrain and Midbrain of the Adult Bullfrog, <i>Rana catesbeiana</i>

Simmons¹,

Horowitz²,

Brown

2007

Brain Behav Evol

View full text Add to dashboard Cite

The distribution of proliferating cells in the midbrain, thalamus, and telencephalon of adult bullfrogs (Rana catesbeiana) was examined using immunohistochemistry for the thymidine analog 5-bromo-2′-deoxyuridine (BrdU) and DNA dot-blotting. At all time points examined (2 to 28 days post-injection), BrdU-labeled cells were located in ventricular zones at all levels of the neuraxis, but with relatively more label around the telencephalic ventricles. Labeled cells, some showing profiles indicative of dividing and migrating cells, were present in brain parenchyma from 7 to 28 days post-injection. These labeled cells were particularly numerous in the dorsal and ventral hypothalamus, preoptic area, optic tectum, and laminar and principal nuclei of the torus semicircularis, with label also present, but at qualitatively reduced levels, in thalamic and telencephalic nuclei. Double-label immunohistochemistry using glial and early neural markers indicated that gliogenesis and neurogenesis both occurred, with new neurons observed particularly in the hypothalamus, optic tectum, and torus semicircularis. In all brain areas, many cells not labeled with BrdU were nonetheless labeled with the early neural marker TOAD-64, indicating that these cells were postmitotic. Incorporation of DNA measured by dot-blotting confirms the presence of DNA synthesis in the forebrain and brainstem at all time points measured. The pattern of BrdU label confirms previous experiments based on labeling with ³H-thymidine and proliferating cell nuclear antigen showing cell proliferation in the adult ranid brain, particularly in hypothalamic nuclei. The consistent appearance of new cells in the hypothalamus of adult frogs suggests that proliferative activity may be important in mediating reproductive behaviors in these animals.

show abstract

“…[46][47][48] This raises the possibility that engrafted FV-vector-transduced neural progenitor cells transduced with genes controlled by the Gfa2 promoter could migrate to diseased areas of the brain, where differentiation into astrocytes could induce the expression of therapeutically relevant gene products.…”

Section: Foamy Viral Transduction Of Neural Progenitor Cells I Rothenmentioning

confidence: 99%

Transduction of human neural progenitor cells with foamy virus vectors for differentiation-dependent gene expression

et al. 2008

View full text Add to dashboard Cite

Neural progenitor cells are potential vehicles for delivery of therapeutic agents into the brain. Differentiation-dependent promoters may be useful to target the therapeutic transgene expression to specific neural cell types. Here we explored the potential of vectors based on the foamy virus (FV) for genetic engineering of neural progenitor cells. We demonstrate that FV vectors can mediate stable long-term constitutive expression of the enhanced green fluorescent protein (EGFP) in neural progenitor cells. For differentiation-dependent gene expression, we constructed a FV vector with an internal expression cassette containing the human 2.2 kb promoter (Gfa2) of the astrocyte-specific glial fibrillary acidic protein (GFAP) and sequences encoding EGFP. We show FV-vector-mediated delivery of the Gfa2-egfp transgene into the human neural stem cell line HNSC.100 and differentiation-dependent expression in stably transduced cell populations. Differentiation of the FV-transduced HNSC.100 cells to astrocytes upregulated expression of both the Gfa2-egfp transgene and the native gfap gene, confirming differentiation-dependent activation of the transduced Gfa2 promoter. These results demonstrate that differentiation-dependent gene expression can be achieved by FV-vector-mediated gene transfer to neural progenitor cells. Our findings support the use of FV vectors for the genetic engineering of neural progenitor cells for therapeutic and research applications.

show abstract

Reactive astrogliosis induces astrocytic differentiation of adult neural stem/progenitor cells in vitro

Cited by 38 publications

References 46 publications

Adult neurogenesis and brain regeneration in zebrafish

Adult neurogenesis and brain regeneration in zebrafish

Cell Proliferation in the Forebrain and Midbrain of the Adult Bullfrog, <i>Rana catesbeiana</i>

Transduction of human neural progenitor cells with foamy virus vectors for differentiation-dependent gene expression

Contact Info

Product

Resources

About