Tricholepis glaberrima DC (Compositae) is popularly known as 'brahmadandi' in Ayurveda, the Indian system of medicine. Aerial parts of this plant are used as a nervine tonic, and its infusion was prescribed in seminal debility and impotence. In the current study, we have investigated the effect of methanol extract of the aerial parts in sexually active male rats. In addition, the effect of the extract on the testicular histology and on the activity of two antioxidant enzymes, viz. superoxide dismutase and catalase in testicular homogenate were determined. Administration of the methanol extract at 200 mg=kg body weight for 28 days altered significantly the various components of the sexual behavior study. The extract increased significantly mounting latency (ML) and intromission latency (IL) with a significant reduction in mounting frequency (MF), intromission frequency (IF), and post-ejaculatory interval (PEI). Examination of the testicular histology revealed that the extract favors spermatogenesis by enhancing the proliferation of the seminiferous epithelium. Further, a significant increase in the activity of superoxide dismutase and catalase was observed in rats treated with methanol extract of T. glaberrima. Therefore, the current findings provide experimental evidence that the extract of T. glaberrima possesses aphrodisiac properties.