Reactivation of temperature-sensitive and non-temperature-sensitive infectious bovine rhinotracheitis vaccine virus with dexamethasone

Abstract

Latent infections by a temperature-sensitive (ts) infectious bovine rhinotracheitis virus vaccines was produced as frequently as by non-ts vaccine virus. Thus virus could be reactivated in seven of eight ts vaccinates and six of eight non-ts vaccinates after dexamethasone treatment. Virus excretion could be detectable for 1 to 8 days at a level of 2 x 10(6) to 3 x 10(8) plaque-forming units per ml of nasal secretions. The reactivated virus was shown to be the same as the original virus used for vaccination by its inability to grow at the restrictive temperature (39 degrees C) as well as by its restriction endonuclease cleavage pattern.

A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates

“…13,29 Like other herpesviruses, BoHV-1 is able to establish life-long latent infection after primary infection, which can be reactivated under natural or experimental conditions. 27,28 All BoHV-1 strains isolated belong to one viral species and are classified into 3 subtypes: 1.1, 1.2a, and 1.2b. 25 Several countries, and some counties within countries, are recognized as IBR-free.…”

A combined bovine herpesvirus 1 gB-gD DNA vaccine induces immune response in mice

“…Like other alphaherpesviruses, BHV-1 establishes latent infections and can be reactivated with subsequent viral re-excretion by several stimuli, including transport, parturition and treatment with glucocorticoids [6].…”