Reactivation of Ribonucleic Acid Synthesis during Early Germination of Agrostemma githago Embryos

Hecker, Michael; Köhler, Karl-Hermann

doi:10.1016/s0015-3796(17)30329-3

Cited by 13 publications

(3 citation statements)

References 25 publications

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“…The pattern of RNA labelling, observed in the lentil embryo during the first 3 h of germination, strongly suggests that activation of the synthesis of the major RNA species occurs simultaneously very soon after the start of imbibition. This result is in agreement with the observations made in cotyledons of Phaseolus vulgaris (Watanabe et al 1973), axes of Triticum vuigare (Doshchanov et al 1975), etnbryos of Agrostemma githago (Hecker et al 1977), axes and cotyledons of Brassica napus (Payne et al 1978), and axes of Pisum sativum (Takaiwa and Tanifuji 1979). In these experimental systems, the synthesis of the major RNA species was initiated simultaneously and a large proportion of the RNA synthesized very early consisted of heterogeneous molecules.…”

Section: Discussionsupporting

confidence: 90%

“…reported for isolated rye embryos (Sen et al 1975), On It is well established that transcription is also acti-the other hand, studies done with various plant species vated during the satne period (Tanifuji et al 1969, have indicated that all major RNA species were simul-Walton and Soofi 1969, Van de Walle and Bernier taneously synthesized after the start of imbibition (Van 1969, Rejman andBuckowicz 1971) and many studies Onckelen et al 1974, Spiegel et al 1975 have been undertaken to identify the newly synthesized et al 1975, Delseny et al 1977a, Hecker et al 1977, RNA species. Conflicting results have often been pre- Payne et al 1978, Takaiwa andTanifuji 1979).…”

Section: Introductionmentioning

confidence: 99%

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Species of RNA synthesized during early germination in the radicle of the lentil embryo

Walle

Deltour

Forgeur

1983

Physiologia Plantarum

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RNA synthesis is activated in cells of the plant embryo very soon after the start of imbibition by the seed. This study was undertaken to determine whether synthesis of one particular RNA or all the major RNA species was initially activated in the radicle of lentil embryos (Vicia lens). Two different methods were used after incorporation of radioactive precursor to identify the newly synthesized RNA species. First, RNA was extracted and analyzed using gel electrophoresis, gel filtration, affinity chromatography and base composition analysis. The second method was to localize the labelled RNA molecules within cells using autoradiography of sections of embryonic radicles. The data indicate that newly synthesized heterogeneous nuclear RNA and possibly messenger RNA, transfer RNA, 5S ribosomal RNA and precursor of ribosomal RNA are detectable 3 h after the start of imbibition of the decoated embryo and before completion of initial water uptake. It is concluded that synthesis of all major species of RNA is simultaneously initiated in the radicle of the germinating embryo.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Species of RNA synthesized during early germination in the radicle of the lentil embryo

Walle

Deltour

Forgeur

1983

Physiologia Plantarum

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“…In an anaerobic environment, ethanol is produced which may cause the self-poisoning of the seed. Furthermore, soaking has also been reported to slow down the synthesis of the RNA and protein in an Agrostemma githago embryo [ 30 ]. However, for other seeds, such as rice, oat, pea, and lettuce, soaking has little effect [ 13 ].…”

Section: Seed Germinationmentioning

confidence: 99%

Effect of Germination on Seed Protein Quality and Secondary Metabolites and Potential Modulation by Pulsed Electric Field Treatment

Bagarinao,

King,

Leong

et al. 2024

Foods

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Plant-based foods are being increasingly favored to feed the ever-growing population, but these need to exhibit improved nutritional value in terms of protein quality and digestibility to be considered a useful alternative to animal-based foods. Germination is essential for plant growth and represents a viable method through which the protein quality of plants can be further improved. However, it will be a challenge to maintain efficient rates of germination in a changing climate when seeds are sown. In the context of the indoor germination of seeds for food, consumption, or processing purposes, a more efficient and sustainable process is desired. Therefore, novel techniques to facilitate seed germination are required. Pulsed electric fields (PEF) treatment of seeds results in the permeabilization of the cell membrane, allowing water to be taken up more quickly and triggering biochemical changes to the macromolecules in the seed during germination. Therefore, PEF could be a chemical-free approach to induce a stress response in seeds, leading to the production of secondary metabolites known to exert beneficial effects on human health. However, this application of PEF, though promising, requires further research to optimize its impact on the protein and bioactive compounds in germinating seeds.

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RNA Polymerase and Regulation of Transcription

Wollgiehn¹

1982

Nucleic Acids and Proteins in Plants II

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Reactivation of Ribonucleic Acid Synthesis during Early Germination of Agrostemma githago Embryos

Cited by 13 publications

References 25 publications

Species of RNA synthesized during early germination in the radicle of the lentil embryo

Species of RNA synthesized during early germination in the radicle of the lentil embryo

Effect of Germination on Seed Protein Quality and Secondary Metabolites and Potential Modulation by Pulsed Electric Field Treatment

RNA Polymerase and Regulation of Transcription

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