Reactivation of p53 by RITA Induces Apoptosis in Human Oral Squamous Cell Carcinoma Cells

Nishioka, Takashi; Numazaki, Kento; Hasegawa, Hiroshi; Takahashi, Tetsu; SUGAWARA, SHUNJI; TADA, HIROYUKI

doi:10.21873/anticanres.15775

Cited by 4 publications

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“…Chemotherapeutic responses and survival rates were depicted to be higher in patients receiving enoxaparin combined with standard therapy 4 . Heparin was determined to reduce cell viability and apoptosis in three out of four oral cancer cell lines utilized 5 . Our study is the first to assess enoxaparin in an orthotopic oral cancer mice model.…”

Section: Discussionmentioning

confidence: 89%

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The effects of enoxaparin treatment in a xenograft mouse model of oral squamous cell carcinoma: A pilot study

Ekici,

Soluk‐Tekkesin,

Küçüksezer

et al. 2024

J Oral Pathology Medicine

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BackgroundRecent studies suggest that enoxaparin may have therapeutic effects on oral squamous cell carcinoma. We aimed to assess this effect utilizing xenograft mouse model through evaluations of proliferation and angiogenesis markers at the RNA and protein levels.MethodsMice were divided into enoxaparin treatment (n = 4), positive control (n = 4) and negative control (n = 3) groups. Immunohistochemical analyses were performed utilizing Bcl‐2, Bax and Ki‐67 antibodies. Expression levels of proliferation and apoptosis related genes were calculated utilizing qRT‐PCR. Time‐dependent proliferation assays were performed in OSC‐19 and HEK293 cell‐lines.ResultsBax antibody showed positive staining in the cytoplasm and nuclei of tumor cells, while Bcl‐2 antibody displayed staining only in the cytoplasm. A proliferation index of 15%–20% was found in all groups with the Ki‐67 marker indicating no metastasis. Enoxaparin treatment caused decrease in BCL2, BAX and CCNB1 genes' expressions. Compared to HEK293, proliferation assays demonstrated higher division rates in OSC‐19 with a significant decrease in viability after 96 h.ConclusionReduced BCL‐2 expression indicates a regression of tumor growth, but reduced BAX expression is not correlated with increased apoptosis. Despite the aggressive nature of OSC‐19, our results showed a low cell viability with a high division rate when compared with the control HEK293. This paralleled our in vivo findings that showed absence of lymph node metastasis across all mice groups. This discrepancy with the literature suggests that further investigations of the underlying mechanisms and protein‐level analyses are needed to draw definitive conclusions about the effect of enoxaparin on OSC‐19 behavior.

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Section: Discussionmentioning

confidence: 89%

“…4 Heparin was determined to reduce cell viability and apoptosis in three out of four oral cancer cell lines utilized. 5 Our study is the first to assess enoxaparin in an orthotopic oral cancer mice model. A similar study evaluated the impact of heparin in a xenograft tumor mouse model that was created utilizing OSC-4 cells.…”

Section: Discussionmentioning

confidence: 96%

The effects of enoxaparin treatment in a xenograft mouse model of oral squamous cell carcinoma: A pilot study

Ekici,

Soluk‐Tekkesin,

Küçüksezer

et al. 2024

J Oral Pathology Medicine

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“…На сьогодні є дослідження щодо здатності низькомолекулярних гепаринів інгібувати пухлинний ангіогенез, проліферацію лінії клітин OSCC [10]. Припускають, що антипроліферативний ефект гепарину зумовлений його антиангіогенною активністю за рахунок перешкоджання утворення тромбіну, інгібування тканинного фактора (TF) та утворення фібрину [11]. Також низькомолекулярні гепарини є основою лікування тромбоемболії пов'язаної з онкопроцесом, але досі не достатньо вивчено нових властивостей антикоагулянтів прямої дії щодо впливу на процеси метастазування, ефективність протипухлинної терапії за умов їх сумісного застосування з протипухлинними препаратами.…”

Section: клінічна та експериментальна медицина / Clinical and Experim...unclassified

“…To date, there have been studies on the ability of low-molecular-weight heparins to inhibit tumor angiogenesis and proliferation of the OSCC cell line [10]. It is suggested that the antiproliferative effect of heparin is due to its antiangiogenic activity by preventing thrombin formation, inhibiting tissue factor (TF) and fibrin formation [11]. Low-molecular-weight heparins are also the basis for the treatment of cancer-related thromboembolism, but new properties of direct-acting anticoagulants in terms of their effect on metastasis and the effectiveness of anticancer therapy when used in combination with anticancer drugs have not yet been sufficiently studied.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Anticoagulant Enoxaparin Direct Action on the Metastatic Activity of Lewis Lung Carcinoma

Munko

2024

VPBM

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Вісник проблем біології і медицини -2024 -Вип. 1 (172) / Bulletin of problems in biology and medicine -2024 -Issue 1 (172) 194 КЛІНІЧНА ТА ЕКСПЕРИМЕНТАЛЬНА МЕДИЦИНА / CLINICAL AND EXPERIMENTAL MEDICINE nodes, conjunctivitis and Koplik's spots were less often diagnosed, but manifestations of intestinal syndrome were more often recorded than during the outbreak in 2006.

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Novel lncRNA 803 related to Marek’s disease inhibits apoptosis of DF-1 cells

Han,

Zhao,

Ren

et al. 2024

Avian Pathology

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Reactivation of p53 by RITA Induces Apoptosis in Human Oral Squamous Cell Carcinoma Cells

Cited by 4 publications

References 0 publications

The effects of enoxaparin treatment in a xenograft mouse model of oral squamous cell carcinoma: A pilot study

The effects of enoxaparin treatment in a xenograft mouse model of oral squamous cell carcinoma: A pilot study

Effect of the Anticoagulant Enoxaparin Direct Action on the Metastatic Activity of Lewis Lung Carcinoma

Novel lncRNA 803 related to Marek’s disease inhibits apoptosis of DF-1 cells

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