Reactivation by dithiols of enzymes inhibited by lewisite

Barrón, E. S. Guzmán; Miller, Zelma; Bartlett, Grant R.; Meyer, Joe; Singer, Thomas P.

doi:10.1042/bj0410069

Cited by 46 publications

(10 citation statements)

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“…1941), iodine (Meyer, 1945) and quinone (Kuhn & Beinert, 1943) apparently inhibit carboxylase. However, in other experiments, this enzyme is not significantly affected by lewisite oxide (Barron, Miller, Bartlett, Meyer & Singer, 1947;Peters, Sinclair & Thompson, 1946), ethylarsenoxide ), iodoacetate (Green et al 1941 or ethyl iodoacetate (Mackworth, 1948). Inactivation by a single type ofthiol detector may not be conclusive evidence of the presence of thiol groups, as even the very selective mercurial compounds can inactivate enzymes like cytochrome oxidase or catalase which in all probability are not thiols (Kreke, Schaefer, Seibert & Cook, 1950; Seibert, ).…”

mentioning

confidence: 87%

The role of sulphydryl groups of yeast carboxylase

Stoppani

Actis

Deferrari

et al. 1953

Biochemical Journal

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mentioning

confidence: 87%

The role of sulphydryl groups of yeast carboxylase

Stoppani

Actis

Deferrari

et al. 1953

Biochemical Journal

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“…Sulfhydryl (SH)-binding agents such as PMB (22,23), GSSG (20,21), and Na arsenite (23)(24)(25)(26) at concentrations of 2 to 5 X 10-2 M abolished agglutinating activity. Ultracentrifugation patterns of the serum remained unchanged ( Figure 3) except for loss of the 28.8 S20,w peak from the Na arsenite-treated sample.…”

Section: Stndies Of the Serum Factormentioning

confidence: 99%

Agglutination of Stored Erythrocytes by a Human Serum. Characterization of the Serum Factor and Erythrocyte Changes*

Ozer¹,

Chaplin²

1963

J. Clin. Invest.

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There have been four previous reports of human sera causing agglutination of stored but not of fresh red cells (1-4). The sera are of special interest for two reasons. Since the agglutinins are active against the patients' own stored cells as well as against cells from other donors, one must consider whether such patients represent examples of autoimmune disease. Of more immediate practical importance, the sera provide a new means for studying the red-cell storage defect, since the agglutinins detect in the erythrocyte membrane a hitherto unrecognized alteration that progresses during in vitro storage concurrently with deterioration in cellular metabolism.Characterization of the previously reported stored-cell agglutinins has been limited. The present communication describes detailed studies of a serum causing strong agglutination of stored human red cells. The serum factor responsible for the agglutination was shown to be a gamma macroglobulin. Studies of red cells during storage in vitro indicate that development of agglutinability is closely related to the alterations in erythrocyte glucose metabolism occurring during storage. Also included are the results of sixteen studies of erythrocyte survival in vivo that are designed to examine the relationship of agglutinability to nonviability of the stored cells. METHODSThe patient was a 71-year-old woman whose chief complaints were abdominal swelling and progressive weakness. The principal positive physical findings were pallor and hepatosplenomegaly. Laboratory findings of special interest were: hemoglobin, 9.7 g per 100 ml; reticulocytes, 4.2%o; serum bilirubin concentration, nor-* Work supported by U. S. Public Health Service research grant G-2918 (C5) from the National Cancer Institute, Bethesda, Md. mal; total serum protein concentration, 7 g per 100 ml (albumin, 2.7 g, globulin, 4.3 g). Survival in vivo of Cr51-labeled, f resh, compatible, red cells of normal donors was moderately severely reduced, judged by redcell life-span (Cr51 tt, about 8 days) based on daily blood sampling for 1 week. Exploratory laparotomy revealed moderate hepatic enlargement, diffuse mesenteric and retroperitoneal lymph-node enlargement, and massive splenomegaly. Splenectomy was performed, and the patient's postoperative course was uneventful. Two units of blood were transfused before and two additional units during surgery. Although the patient was improved at the time of discharge and required no further transfusions, she died at home 7 months later of undetermined cause; necroscopy was not performed. The spleen removed at operation weighed 2,050 g and showed hyperplasia of the white pulp, with impaired follicle formation; each Malpighian corpuscle was surrounded by a margin of hyperplastic lymphoid cells. Lymph nodes revealed intact architecture, but were filled out with lymphoid elements without follicle formation. A liver sample from biopsy was not remarkable, and aspirated bone marrow revealed only generalized hyperplasia. A specific pathological diagnosis was not made. Stndies...

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“…Not too many years following the discovery of salvarsan, the scientific principles underlying its action were applied to the "rational" design of a war gas. 2-Chlorovinyldichloroarsine, named lewisite in honor of Lewis, the American chemist who synthesized it (Lewis and Steigler, 1925), is hydrolyzed by body fluids to the corresponding arsine oxide (Figure 5-4) (Peters et al, 1945;Stocken and Thompson, 1946;Stocken eta/., 1947;Waters and Stock, 1945 oxides react readily with SH groups, and are powerful inhibitors of SHcontaining enzymes (Barron et a/., 1947). The ability of arsenic compounds such as diphenylchloroarsine to abolish the so-called "fixed sulfhydryls"of tissues was discovered in the 1920s.…”

Section: Metal Chelationmentioning

confidence: 99%

Thiols, Disulfides, and Thioesters

Huxtable

1986

Biochemistry of Sulfur

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Reactivation by dithiols of enzymes inhibited by lewisite

Cited by 46 publications

References 2 publications

The role of sulphydryl groups of yeast carboxylase

The role of sulphydryl groups of yeast carboxylase

Agglutination of Stored Erythrocytes by a Human Serum. Characterization of the Serum Factor and Erythrocyte Changes*

Thiols, Disulfides, and Thioesters

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