Reactions of the bioregulatory agent nitric oxide in oxygenated aqueous media: Determination of the kinetics for oxidation and nitrosation by intermediates generated in the nitric oxide/oxygen reaction

Wink, David A.; Darbyshire, John F.; Nims, Raymond W.; Saavedra, Joseph E.; Ford, Peter C.

doi:10.1021/tx00031a003

Cited by 483 publications

(314 citation statements)

References 23 publications

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“…The half-life of • NO in pure water is inversely proportional to its concentration [29] and at a concentration of 0.05-1 μM ranges from 500 s to several hours [32]. Nitrite is the only stable final product of • NO autooxidation in an aqueous solution (Fig.…”

Section: Discussionmentioning

confidence: 99%

Heat-induced formation of nitrogen oxides in water

2013

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It was found by the fluorimetric method using 2,3-diaminonaphthalene that moderate heating of water (60-80 • C, for up to 4 h) leads to the fixation of atmospheric nitrogen with the formation of nitrite. The kinetic parameters of this process were determined. The energy of activation of NO − 2 formation was estimated to be 139 kJ/mol. It was found that the amount of nitrite formed depends on the concentration of dissolved oxygen and nitrogen. It was shown by two independent methods (Griess reagent/VCl 3 and 2,3-diaminonaphthalene/nitrate reductase) that heating of water (80 • C, 1 h) results in the formation of nitrate; with the use of the fluorescent probe dihydrorhodamine 123, the generation of nitrogen dioxide (peroxynitrite) was revealed. Nitrite, nitrate, and nitrogen dioxide are formed in water upon heating in approximately equal amounts. A scheme of reactions proceeding with bidistilled water by the action of heat with the formation of nitrogen oxides is proposed.

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Section: Discussionmentioning

confidence: 99%

Heat-induced formation of nitrogen oxides in water

2013

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“…Finally, we emphasize that all these treatments were performed aerobically, which means that the effects (both induction of resistance and cellular damage) may be due to multiple reactive nitrogen oxide species in addition to • NO [42]. Protection may involve a general response to oxidative injury, which could explain the cross-resistance imparted to H202 toxicity.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen oxide‐induced autoprotection in isolated rat hepatocytes

Kim

Bergonia

Lancaster³

1995

FEBS Letters

197

110

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Pretreatment of rat hepatocytes with low-dose nitrogen oxide (addition of SNAP in vitro or induction of nitric oxide synthase in vitro or in vivo) imparts resistance to killing and decrease in aconitase and mitochondrial electron transfer from a second exposure to a higher dose of SNAP. Induction of this resistance is prevented by cycloheximide, indicating upregulation of protective protein(s). Ferritin levels are increased as are nonheme iron-NO EPR signals. Tin-protoporphyrin (SnPP) prevents protection, suggesting involvement of hsp32 (heme oxygenase) and/or guanylyl cyclase (GC). Cross-resistance to H2Oz killing is also observed, which is also prevented by cycloheximide and SnPP. Thus, hepatocytes possess inducible protective mechanisms against nitrogen oxide and reactive oxygen toxicity.

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“…However, peroxynitrite (ONOO − ) and nitrogen dioxide (NO 2 ), which can be formed from the reaction of NO with superoxide (O 2 − ), are also potent oxidants (> 1.0 V NHE) 22 . In contrast, N 2 O 3 formed from the reaction of NO with O 2 (autoxidation), as well as the NO/O 2 − reaction is a mild oxidant and prefers to nitrosate nucleophiles such as amines and thiols [23][24][25] . The balance between oxidation and nitrosation chemistry as it was found depends largely on the flux of NO (Fig.…”

Section: Introductionmentioning

confidence: 99%

The chemical biology of nitric oxide: Implications in cellular signaling

Thomas¹,

Ridnour²,

Isenberg³

et al. 2008

Free Radical Biology and Medicine

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808

697

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Nitric oxide (NO) has earned the reputation of being a signaling mediator with many diverse and often opposing biological activities. The diversity in response to this simple diatomic molecule comes from the enormous variety of chemical reactions and biological properties associated with it. In the last few years, the importance of steady state NO concentrations have emerged as a key determinant of its biological function. Precise cellular responses are differentially regulated by specific NO concentration. We propose 5 basic distinct concentration levels of NO activity; cGMP mediated processes ([NO] <1-30 nM; Akt phosphorylation ([NO] = 30-100 nM); stabilization of HIF-1α ([NO] = 100-300 nM); phosphorylation of p53 ([NO] > 400 nM) and nitrosative stress (1 µM). In general, lower NO concentrations promote cell survival and proliferation, while higher levels favor cell cycle arrest, apoptosis, and senescence. Free radical interactions will also influence NO signaling. One of the consequences of reactive oxygen species (ROS) generation is to reduce NO concentrations. This antagonizes the signaling of nitric oxide and in some cases results in converting a cell cycle arrest profile to a cell survival one. The resulting reactive nitrogen species (RNS) that are generated from these reactions can also have biological effects and increase oxidative and nitrosative stress responses. A number of factors determine the formation of NO and its concentration, such as diffusion, consumption, and substrate availability which are referred to as Kinetic Determinants for Molecular Target Interactions. These are the chemical and biochemical parameters that shape cellular responses to NO. Herein we discuss signal transduction and the chemical biology of NO in terms of the direct and indirect reactions.

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Reactions of the bioregulatory agent nitric oxide in oxygenated aqueous media: Determination of the kinetics for oxidation and nitrosation by intermediates generated in the nitric oxide/oxygen reaction

Cited by 483 publications

References 23 publications

Heat-induced formation of nitrogen oxides in water

Heat-induced formation of nitrogen oxides in water

Nitrogen oxide‐induced autoprotection in isolated rat hepatocytes

The chemical biology of nitric oxide: Implications in cellular signaling

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